dc.contributor.author | Lyu, Chuang | |
dc.contributor.author | Lyu, Gong-Wei | |
dc.contributor.author | Mulder, Jan | |
dc.contributor.author | Martinez, Aurora | |
dc.contributor.author | Shi, Tie-Jun | |
dc.date.accessioned | 2021-08-05T09:06:40Z | |
dc.date.available | 2021-08-05T09:06:40Z | |
dc.date.created | 2020-06-04T19:46:27Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1178-7090 | |
dc.identifier.uri | https://hdl.handle.net/11250/2766396 | |
dc.description.abstract | Background: G protein-gated inwardly rectifying potassium (GIRK) channels are involved in the regulation of neuronal excitability. Four GIRK subunits (GIRK1-4) are expressed in rat dorsal root ganglia (DRGs). Recently, we have characterized the expression of GIRK1 and − 2, and both are downregulated in rat DRGs and spinal cord after a complete sciatic nerve transection (axotomy). Here, we aimed to study the neurochemical characteristics of GIRK3, and its regulation in rat DRGs and spinal cord induced by nerve injury.
Methods: A sciatic nerve axotomy was performed to study the influences of injury on GIRK3 expression in DRGs and spinal cord. A dorsal root rhizotomy and a sciatic nerve crush were employed to study the axonal transport of GIRK3 protein, respectively. Immunohistochemistry analysis was employed for investigating the neurochemical characteristics of GIRK3.
Results: In control DRGs, ∼ 18% of neuron profiles (NPs) were GIRK3-positive (+), and ∼ 41%, ∼ 48% and ∼ 45% of GIRK3+ NPs were CGRP+, IB4+ and NF200+, respectively. GIRK3-like immunoreactivity was observed in glabrous skin of hind paws and axons originating from DRG neurons. Fourteen days after axotomy, more than one-third of DRG NPs were GIRK3+, and among these ∼ 51% and ∼ 56% coexpressed galanin and neuropeptide Y, respectively. In control animals, a small group of interneurons found in the dorsal horn was GIRK3+. In addition, GIRK3+ processes could be observed in superficial laminae of spinal dorsal horn. After nerve injury, the intensity of GIRK3-like immunoreactivity in the superficial layers was increased. Evidence based on rhizotomy and sciatic nerve crush indicated both anterograde and retrograde transport of GIRK3.
Conclusion: Our study demonstrates that GIRK3 is expressed in sensory neurons and spinal cord. GIRK3 has both anterograde and retrograde axonal transport. GIRK3 expression can be regulated by peripheral nerve injury. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | DovePress | en_US |
dc.relation.uri | https://www.dovepress.com/g-protein-gated-inwardly-rectifying-potassium-channel-subunit-3-is-upr-peer-reviewed-fulltext-article-JPR | |
dc.rights | Navngivelse-Ikkekommersiell 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/deed.no | * |
dc.title | G Protein-Gated Inwardly Rectifying Potassium Channel Subunit 3 Is Upregulated in Rat DRGs and Spinal Cord After Peripheral Nerve Injury | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2020 The Authors | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.2147/JPR.S233744 | |
dc.identifier.cristin | 1813936 | |
dc.source.journal | Journal of Pain Research | en_US |
dc.source.pagenumber | 419-429 | en_US |
dc.relation.project | Norges forskningsråd: 261826 | en_US |
dc.relation.project | Norges forskningsråd: 248889 | en_US |
dc.identifier.citation | Journal of Pain Research. 2020, 13, 419-429. | en_US |
dc.source.volume | 13 | en_US |