Now showing items 1-3 of 3
Filtering duplicate reads from 454 pyrosequencing data
(Oxford University Press, 2013)
Motivation: Throughout the recent years, 454 pyrosequencing has emerged as an efficient alternative to traditional Sanger sequencing and is widely used in both de novo whole-genome sequencing and metagenomics. Especially ...
Systematic exploration of error sources in pyrosequencing flowgram data
(Oxford University Press, 2011)
Motivation: 454 pyrosequencing, by Roche Diagnostics, has emerged as an alternative to Sanger sequencing when it comes to read lengths, performance and cost, but shows higher per-base error rates. Although there are ...
Characteristics of 454 pyrosequencing data—enabling realistic simulation with flowsim
(Oxford University Press, 2010)
Motivation: The commercial launch of 454 pyrosequencing in 2005was a milestone in genome sequencing in terms of performance and cost. Throughout the three available releases, average read lengths have increased to ∼500 ...