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dc.contributor.authorKristoffersen, Arne Skodvin
dc.contributor.authorHamre, Børge
dc.contributor.authorFrette, Øyvind
dc.contributor.authorErga, Svein Rune
dc.date.accessioned2016-03-07T14:11:26Z
dc.date.available2016-03-07T14:11:26Z
dc.date.issued2015
dc.PublishedEuropean Biophysics Journal 2015eng
dc.identifier.issn0175-7571en_US
dc.identifier.urihttps://hdl.handle.net/1956/11460
dc.description.abstractThe fluorescence lifetime is a very useful parameter for investigating biological materials on the molecular level as it is mostly independent of the fluorophore concentration. The green alga Tetraselmis blooms in summer, and therefore its response to UV irradiation is of particular interest. In vivo fluorescence lifetimes of chlorophyll a were measured under both normal and UV-stressed conditions of Tetraselmis. Fluorescence was induced by two-photon excitation using a femtosecond laser and laser scanning microscope. The lifetimes were measured in the time domain by time-correlated single-photon counting. Under normal conditions, the fluorescence lifetime was 262 ps, while after 2 h of exposure to UV radiation the lifetime increased to 389 ps, indicating decreased photochemical quenching, likely caused by a damaged and down-regulated photosynthetic apparatus. This was supported by a similar increase in the lifetime to 425 ps when inhibiting photosynthesis chemically using DCMU. Furthermore, the UV-stressed sample was dark-adapted overnight, resulting in a return of the lifetime to 280 ps, revealing that the damage caused by UV radiation is repairable on a relatively short time scale. This reversal of photosynthetic activity was also confirmed by FV/FM measurements.en_US
dc.language.isoengeng
dc.publisherSpringeren_US
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.subjectFluorescence lifetimeeng
dc.subjectUV stresseng
dc.subjectPhotosynthesiseng
dc.subjectChlorophylleng
dc.titleChlorophyll a fluorescence lifetime reveals reversible UV-induced photosynthetic activity in the green algae Tetraselmisen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2015-11-27T13:35:42Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2015 The Authorsen_US
dc.identifier.doihttps://doi.org/10.1007/s00249-015-1092-z
dc.identifier.cristin1294382


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