Show simple item record

dc.contributor.authorKourtesis, Ioannis
dc.contributor.authorKasparov, Sergey
dc.contributor.authorVerkade, Paul
dc.contributor.authorTeschemacher, Anja G.
dc.date.accessioned2016-04-14T13:01:58Z
dc.date.available2016-04-14T13:01:58Z
dc.date.issued2015-10-29
dc.identifier.citationASN Neuro 2015, 7(5: 1759091415610115 )eng
dc.identifier.urihttp://hdl.handle.net/1956/11914
dc.description.abstractThe spontaneously hypertensive rat (SHR) replicates many clinically relevant features of human essential hypertension and also exhibits behavioral symptoms of attention-deficit/hyperactivity disorder and dementia. The SHR phenotype is highly complex and cannot be explained by a single genetic or physiological mechanism. Nevertheless, numerous studies including our own work have revealed striking differences in central catecholaminergic transmission in SHR such as increased vesicular catecholamine content in the ventral brainstem. Here, we used immunolabeling followed by confocal microscopy and electron microscopy to quantify vesicle sizes and populations across three catecholaminergic brain areas—nucleus tractus solitarius and rostral ventrolateral medulla, both key regions for cardiovascular control, and the locus coeruleus. We also studied colocalization of neuropeptide Y (NPY) in norepinephrine and epinephrine-containing neurons as NPY is a common cotransmitter with central and peripheral catecholamines. We found significantly increased expression and coexpression of NPY in norepinephrine and epinephrine-positive neurons of locus coeruleus in SHR compared with Wistar rats. Ultrastructural analysis revealed immunolabeled vesicles of 150 to 650 nm in diameter (means ranging from 250 to 300 nm), which is much larger than previously reported. In locus coeruleus and rostral ventrolateral medulla, but not in nucleus tractus solitarius, of SHR, noradrenergic and adrenergic vesicles were significantly larger and showed increased NPY colocalization when compared with Wistar rats. Our morphological evidence underpins the hypothesis of hyperactivity of the noradrenergic and adrenergic system and increased norepinephrine and epinephrine and NPY cotransmission in specific brain areas in SHR. It further strengthens the argument for a prohypertensive role of C1 neurons in the rostral ventrolateral medulla as a potential causative factor for essential hypertension.eng
dc.language.isoengeng
dc.publisherSageeng
dc.rightsAttribution CC BY 3.0eng
dc.rights.urihttp://creativecommons.org/licenses/by/3.0eng
dc.subjecthypertensioneng
dc.subjectnucleus tractus solitariuseng
dc.subjectrostral ventrolateral medullaeng
dc.subjectlocus coeruleuseng
dc.subjectelectroneng
dc.titleUltrastructural correlates of enhanced norepinephrine and neuropeptide y cotransmission in the spontaneously hypertensive rat braineng
dc.typeJournal articleeng
dc.date.updated2016-03-08T08:15:28Z
dc.rights.holderCopyright The Author(s) 2015eng
dc.type.versionpublishedVersioneng
bora.peerreviewedPeer reviewedeng
dc.type.documentJournal article
dc.identifier.cristinID1333790
dc.identifier.doi10.1177/1759091415610115eng
dc.source.issn1759-0914eng
noa.nsiVDP::Matematikk og Naturvitenskap: 400eng


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution CC BY 3.0
Except where otherwise noted, this item's license is described as Attribution CC BY 3.0