dc.contributor.author | Grudic, Amra | en_US |
dc.contributor.author | Jul-Larsen, Åsne | en_US |
dc.contributor.author | Haring, SJ | en_US |
dc.contributor.author | Wold, MS | en_US |
dc.contributor.author | Lønning, Per Eystein | en_US |
dc.contributor.author | Bjerkvig, Rolf | en_US |
dc.contributor.author | Bøe, Stig Ove | en_US |
dc.date.accessioned | 2016-06-09T09:18:36Z | |
dc.date.available | 2016-06-09T09:18:36Z | |
dc.date.issued | 2007-10-24 | |
dc.Published | Nucleic Acids Research 2007, 35(21):7267-7278 | eng |
dc.identifier.issn | 1362-4962 | |
dc.identifier.uri | https://hdl.handle.net/1956/12084 | |
dc.description.abstract | The activation of a telomere maintenance mechanism is required for cancer development in humans. While most tumors achieve this by expressing the enzyme telomerase, a fraction (5–15%) employs a recombination-based mechanism termed alternative lengthening of telomeres (ALT). Here we show that loss of the single-stranded DNA-binding protein replication protein A (RPA) in human ALT cells, but not in telomerase-positive cells, causes increased exposure of single-stranded G-rich telomeric DNA, cell cycle arrest in G2/M phase, accumulation of single-stranded telomeric DNA within ALT-associated PML bodies (APBs), and formation of telomeric aggregates at the ends of metaphase chromosomes. This study demonstrates differences between ALT cells and telomerase-positive cells in the requirement for RPA in telomere processing and implicates the ALT mechanism in tumor cells as a possible therapeutic target. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Oxford University Press (OUP) | eng |
dc.rights | Attribution CC BY-NC 2.0 UK | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/2.0/uk/ | eng |
dc.title | Replication protein A prevents accumulation of single-stranded telomeric DNA in cells that use alternative lengthening of telomeres | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2016-04-07T09:16:56Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2007 The Author(s) | |
dc.identifier.doi | https://doi.org/10.1093/nar/gkm738 | |
dc.identifier.cristin | 364220 | |
dc.subject.nsi | VDP::Medisinske Fag: 700 | en_US |