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dc.contributor.authorSøreide, Kjetilen_US
dc.contributor.authorWatson, Martin Men_US
dc.contributor.authorLea, Dordien_US
dc.contributor.authorNordgård, Oddmunden_US
dc.contributor.authorSøreide, Jon Arneen_US
dc.contributor.authorHagland, Hanne Ren_US
dc.date.accessioned2016-08-10T09:51:33Z
dc.date.available2016-08-10T09:51:33Z
dc.date.issued2016-06-29
dc.PublishedJournal of Translational Medicine 2016, 14(1):192eng
dc.identifier.issn1479-5876
dc.identifier.urihttps://hdl.handle.net/1956/12539
dc.description.abstractBackground: More accurate predictive and prognostic biomarkers for patients with colorectal cancer (CRC) primaries or colorectal liver metastasis (CLM) are needed. Outside clinical trials, the translational integration of emerging pathways and novel techniques should facilitate exploration of biomarkers for improved staging and prognosis. Methods: An observational study exploring predictive and prognostic biomarkers in a population-based, consecutive cohort of surgically treated colorectal cancers and resected colorectal liver metastases. Long-term outcomes will be cancer-specific survival, recurrence-free survival and overall survival at 5 years from diagnosis. Beyond routine clinicopathological and anthropometric characteristics and laboratory and biochemistry results, the project allows for additional blood samples and fresh-frozen tumour and normal tissue for investigation of circulating tumour cells (CTCs) and novel biomarkers (e.g. immune cells, microRNAs etc.). Tumour specimens will be investigated by immunohistochemistry in full slides. Extracted DNA/RNA will be analysed for genomic markers using specific PCR techniques and next-generation sequencing (NGS) panels. Flow cytometry will be used to characterise biomarkers in blood. Collaboration is open and welcomed, with particular interest in mutual opportunities for validation studies. Status and perspectives: The project is ongoing and recruiting at an expected rate of 120–150 patients per year, since January 2013. A project on circulating tumour cells (CTCs) has commenced, with analysis being prepared. Investigating molecular classes beyond the TNM staging is under way, including characteristics of microsatellite instability (MSI) and elevated microsatellite alterations in selected tetranucleotides (EMAST). Hot spot panels for known mutations in CRC are being investigated using NGS. Immune-cell characteristics are being performed by IHC and flow cytometry in tumour and peripheral blood samples. The project has ethical approval (REK Helse Vest, #2012/742), is financially supported with a Ph.D.-Grant (EMAST project; Folke Hermansen Cancer Fund) and a CTC-project (Norwegian Research Council; O. Nordgård). The ACROBATICC clinical and molecular biobank repository will serve as a long-term source for novel exploratory analysis and invite collaborators for mutual validation of promising biomarker results. The project aims to generate results that can help better discern prognostic groups in stage II/III cancers; explore prognostic and predictive biomarkers, and help detail the biology of colorectal liver metastasis for better patient selection and tailored treatment. The project is registered at http://www.ClinicalTrials.gov NCT01762813.en_US
dc.language.isoengeng
dc.publisherBioMed Centraleng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0eng
dc.subjectBiomarkereng
dc.subjectCancereng
dc.subjectPopulation-basedeng
dc.subjectTranslational researcheng
dc.subjectColorectal cancereng
dc.subjectLiver metastasiseng
dc.subjectCirculating tumour cellseng
dc.subjectGeneticseng
dc.titleAssessment of clinically related outcomes and biomarker analysis for translational integration in colorectal cancer (ACROBATICC): study protocol for a population-based, consecutive cohort of surgically treated colorectal cancers and resected colorectal liver metastasisen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2016-06-29T16:03:07Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2016 The Authors
dc.identifier.doihttps://doi.org/10.1186/s12967-016-0951-4


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