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dc.contributor.authorSulen, Andre
dc.contributor.authorGullaksen, Stein-Erik
dc.contributor.authorBader, Lucius
dc.contributor.authorMcClymont, David
dc.contributor.authorSkavland, Jørn
dc.contributor.authorGavasso, Sonia
dc.contributor.authorGjertsen, Bjørn Tore
dc.date.accessioned2016-12-15T15:34:28Z
dc.date.available2016-12-15T15:34:28Z
dc.date.issued2016-11
dc.identifier.citationPharmacological Research 2016, 113:216-227eng
dc.identifier.urihttp://hdl.handle.net/1956/15243
dc.description.abstractHydrogen sulfide (H2S) is an endogenous gasotransmitter in human physiology and inflammatory disease, however, with limited knowledge of how signal transduction pathways are involved in immune cells. To examine the effects of sulfide on relevant intracellular signaling in human peripheral blood mononuclear cells (PBMCs), we stimulated healthy donor PBMCs with sodium hydrosulfide (NaHS, 1–1000 μM) to mimic H2S stimulation, and analyzed phosphorylation of p38 mitogen activated protein kinase (MAPK) (pT180/pY182), NF-κB p65 (pS529), Akt (pS473) and CREB/ATF1 (pS133/pS63) with flow and mass cytometry. In contrast to transient effects in subsets of lymphocytes, classical monocytes demonstrated sustained phosphorylation of p38, Akt and CREB/ATF1. NaHS induced calcium dependent phosphorylation of p38, Akt and CREB, but not NF-κB, and the phosphorylation of Akt was partly dependent on p38, indicative of p38-Akt crosstalk. Attenuation of these effects by molecules targeting p38 and Hsp90 indicated Hsp90 as a possible target for H2S-induced activation of p38. These results provide a description of a NaHS-induced signal transduction pathway in human primary immune cells that may have relevance for the role of sulfides in inflammation.eng
dc.language.isoengeng
dc.publisherElseviereng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/eng
dc.subjectHydrogen sulfide (H2S)eng
dc.subjectIntracellular signalingeng
dc.subjectPhospho-specific antibodieseng
dc.subjectP38 mitogen activated protein kinase (MAPK)eng
dc.subjectSmall molecule inhibitorseng
dc.subjectCalciumeng
dc.titleSignaling effects of sodium hydrosulfide in healthy donor peripheral blood mononuclear cellseng
dc.typeJournal articleeng
dc.date.updated2016-11-07T10:48:16Z
dc.rights.holderCopyright 2016 The Author(s)eng
dc.type.versionpublishedVersioneng
bora.peerreviewedPeer reviewedeng
dc.type.documentJournal article
dc.identifier.cristinID1394108
dc.identifier.doi10.1016/j.phrs.2016.08.018eng
dc.source.issn1096-1186eng
bora.bpoaIDbpoa945


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