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dc.contributor.authorNuland, Ole Petter
dc.date.accessioned2017-05-19T10:33:55Z
dc.date.available2017-05-19T10:33:55Z
dc.date.issued2009-07-01
dc.date.submitted2009-07-01eng
dc.identifier.urihttp://hdl.handle.net/1956/15878
dc.description.abstractDietary nucleotides and nucleic acids are known to affect immune functions in animals and humans, both in vitro and in vivo. In this thesis I wanted to investigate the effect of dietary RNA from baker’s yeast on cytokine secretion from human peripheral blood mononuclear cells (PBMS). We co‐cultured PBMCs from 11 subjects with RNA, LPS, PHA, SB 202190 and combinations of these, and analyzed supernatants for cytokines using ELISA kits. RNA induced secretion of IL‐1α, IL‐1β, IL‐6, IL‐8, IL‐10, TNF‐α and GM‐CSF, co‐stimulation of RNA increased secretion in PHA stimulated GM‐CSF only. No secretion of IL‐2, IL‐4, IL‐12 or IL‐17α was detected. Inhibition of p38 MAPK with SB 202190 decreased RNA especially, but also LPS and PHA induced cytokine secretions. These results indicate that RNA induce cytokine secretion alone, but has little additive effect on secretion by LPS and PHA stimulation, except for GM‐CSF; and the effect of RNA is partly mediated by p38 MAPK. The stimulatory effect on GM‐CSF might indicate a role for RNA in priming of innate immune response.eng
dc.format.extent1708937 byteseng
dc.format.mimetypeapplication/pdfeng
dc.language.isoengeng
dc.publisherThe University of Bergeneng
dc.subjectdietary nucleotideseng
dc.subjectcytokineseng
dc.subjectPBMCeng
dc.subjectRNAeng
dc.titleDietary RNA (s. Cerevisiae) stimulated cytokine secretion in human immune cells: involvement of p38 MAPK, and co-stimulation with LPS or PHAeng
dc.typeMaster thesiseng
dc.type.degreeMaster i Human ernæringeng
dc.type.courseNUHUM395eng
dc.subject.archivecodeMastergradeng
dc.subject.nus759999eng
dc.type.programMAMD-NUHUMeng
dc.rights.holderCopyright the Author. All rights reservedeng
bora.peerreviewedNot peer reviewedeng


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