Show simple item record

dc.contributor.authorKroksveen, Ann Cathrine
dc.contributor.authorAasebø, Elise
dc.contributor.authorVethe, Heidrun
dc.contributor.authorvan Pesch, Vincent
dc.contributor.authorFranciotta, Diego
dc.contributor.authorTeunissen, Charlotte E.
dc.contributor.authorUlvik, Rune Johan
dc.contributor.authorVedeler, Christian A.
dc.contributor.authorMyhr, Kjell-Morten
dc.contributor.authorBarsnes, Harald
dc.contributor.authorBerven, Frode
dc.date.accessioned2017-11-20T08:33:56Z
dc.date.available2017-11-20T08:33:56Z
dc.date.issued2013-01
dc.identifier.citationKroksveen AC, Aasebø E, Vethe H, van Pesch V, Franciotta D, Teunissen CE, Ulvik RJ, Vedeler CA, Myhr K, Barsnes H, Berven F. Discovery and initial verification of differentially abundant proteins between multiple sclerosis patients and controls using iTRAQ and SID-SRM. Journal of Proteomics. 2013;78:312-325eng
dc.identifier.urihttp://hdl.handle.net/1956/16942
dc.description.abstractIn the present study, we aimed to discover cerebrospinal fluid (CSF) proteins with significant abundance difference between early multiple sclerosis patients and controls, and do an initial verification of these proteins using selected reaction monitoring (SRM). iTRAQ and Orbitrap MS were used to compare the CSF proteome of patients with clinically isolated syndrome (CIS) (n = 5), patients with relapsing–remitting multiple sclerosis that had CIS at the time of lumbar puncture (n = 5), and controls with other inflammatory neurological disease (n = 5). Of more than 1200 identified proteins, five proteins were identified with significant abundance difference between the patients and controls. In the initial verification using SRM we analyzed a larger patient and control cohort (n = 132) and also included proteins reported as differentially abundant in multiple sclerosis in the literature. We found significant abundance difference for 11 proteins after verification, of which the five proteins alpha-1-antichymotrypsin, contactin-1, apolipoprotein D, clusterin, and kallikrein-6 were significantly differentially abundant in several of the group comparisons. This initial study form the basis for further biomarker verification studies in even larger sample cohorts, to determine if these proteins have relevance as diagnostic or prognostic biomarkers for multiple sclerosis.eng
dc.language.isoengeng
dc.publisherElseviereng
dc.rightsAll rights reserved.eng
dc.subjectMultiple sclerosiseng
dc.subjectCerebrospinal fluideng
dc.subjectSelected reaction monitoringeng
dc.subjectQuantitative mass spectrometryeng
dc.titleDiscovery and initial verification of differentially abundant proteins between multiple sclerosis patients and controls using iTRAQ and SID-SRMeng
dc.typeJournal articleeng
dc.subject.nsiVDP::Medisinske fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk biokjemi: 726
dc.subject.nsiVDP::Midical sciences: 700::Basic medical, dental and veterinary sciences: 710::Medical biochemistry: 726
dc.subject.nsiVDP::Medisinske fag: 700::Klinisk medisinske fag: 750::Nevrologi: 752
dc.subject.nsiVDP::Midical sciences: 700::Clinical medical sciences: 750::Neurology: 752
dc.date.updated2017-09-06T14:33:14Z
dc.rights.holderCopyright 2012 Elsevier B.V.eng
dc.type.versionacceptedVersioneng
bora.peerreviewedPeer reviewedeng
bora.journalTitleJournal of Proteomicseng
bibo.volume78eng
bibo.pageStart312eng
bibo.pageEnd325eng
dc.type.documentJournal article
dc.identifier.cristinID1029256
dc.identifier.doi10.1016/j.jprot.2012.09.037eng
dc.source.issn1874-3919eng
dc.source.issn1876-7737eng
dc.relation.projectIDNorges forskningsråd: 204833
noa.nsiVDP::Medisinske Fag: 700eng
dc.relation.journalJournal of Proteomics


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record