dc.contributor.author | Maag, Jesper L.V. | en_US |
dc.contributor.author | Kaczorowski, Dominik C. | en_US |
dc.contributor.author | Panja, Debabrata | en_US |
dc.contributor.author | Peters, Timothy J. | en_US |
dc.contributor.author | Bramham, Clive R. | en_US |
dc.contributor.author | Wibrand, Karin | en_US |
dc.contributor.author | Dinger, Marcel E. | en_US |
dc.date.accessioned | 2018-01-05T10:15:01Z | |
dc.date.available | 2018-01-05T10:15:01Z | |
dc.date.issued | 2017-03-23 | |
dc.Published | Maag JL, Kaczorowski DC, Panja D, Peters, Bramham CRE, Wibrand K, Dinger ME. Widespread promoter methylation of synaptic plasticity genes in long-term potentiation in the adult brain in vivo. BMC Genomics. 2017;18:250 | eng |
dc.identifier.issn | 1471-2164 | |
dc.identifier.uri | https://hdl.handle.net/1956/17140 | |
dc.description.abstract | Background: DNA methylation is a key modulator of gene expression in mammalian development and cellular differentiation, including neurons. To date, the role of DNA modifications in long-term potentiation (LTP) has not been explored. Results: To investigate the occurrence of DNA methylation changes in LTP, we undertook the first detailed study to describe the methylation status of all known LTP-associated genes during LTP induction in the dentate gyrus of live rats. Using a methylated DNA immunoprecipitation (MeDIP)-array, together with previously published matched RNA-seq and public histone modification data, we discover widespread changes in methylation status of LTP-genes. We further show that the expression of many LTP-genes is correlated with their methylation status. We show that these correlated genes are enriched for RNA-processing, active histone marks, and specific transcription factors. These data reveal that the synaptic activity-evoked methylation changes correlates with pre-existing activation of the chromatin landscape. Finally, we show that methylation of Brain-derived neurotrophic factor (Bdnf) CpG-islands correlates with isoform switching from transcripts containing exon IV to exon I. Conclusions: Together, these data provide the first evidence of widespread regulation of methylation status in LTP-associated genes. | en_US |
dc.language.iso | eng | eng |
dc.publisher | BioMed Central | eng |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0 | eng |
dc.subject | Long-term potentiation | eng |
dc.subject | LTP | eng |
dc.subject | DNA-methylation | eng |
dc.subject | MeDIP | eng |
dc.subject | Synaptic plasticity | eng |
dc.subject | Epigenetics | eng |
dc.subject | Neuroepigenetics | eng |
dc.title | Widespread promoter methylation of synaptic plasticity genes in long-term potentiation in the adult brain in vivo. | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2017-12-07T13:46:32Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2017 The Author(s) | |
dc.identifier.doi | https://doi.org/10.1186/s12864-017-3621-x | |
dc.identifier.cristin | 1481211 | |
dc.source.journal | BMC Genomics | |
dc.relation.project | Norges forskningsråd: 204861 | |
dc.relation.project | Norges forskningsråd: 199355 | |