BORA - UiBBORA
    • English
    • norsk
  • English 
    • English
    • norsk
  • Login
View Item 
  •   BORA Home
  • Faculty of Medicine
  • Department of Biomedicine
  • Department of Biomedicine
  • View Item
  •   BORA Home
  • Faculty of Medicine
  • Department of Biomedicine
  • Department of Biomedicine
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

AMPA receptors at ribbon synapses in the mammalian retina: kinetic models and molecular identity

Type
Journal article
Peer reviewed
acceptedVersion
Thumbnail
View/Open
Accepted version (9.316Mb)

Under embargo until: 21.09.2018

Date
2017
Author
Hartveit, Espen
Zandt, Bas-Jan
Madsen, Eirik
Castilho, Aurea
Mørkve, Svein Harald
Veruki, Margaret Lin
Share

Metadata
Show full item record
Abstract
In chemical synapses, neurotransmitter molecules released from presynaptic vesicles activate populations of postsynaptic receptors that vary in functional properties depending on their subunit composition. Differential expression and localization of specific receptor subunits are thought to play fundamental roles in signal processing, but our understanding of how that expression is adapted to the signal processing in individual synapses and microcircuits is limited. At ribbon synapses, glutamate release is independent of action potentials and characterized by a high and rapidly changing rate of release. Adequately translating such presynaptic signals into postsynaptic electrical signals poses a considerable challenge for the receptor channels in these synapses. Here, we investigated the functional properties of AMPA receptors of AII amacrine cells in rat retina that receive input at spatially segregated ribbon synapses from OFF-cone and rod bipolar cells. Using patch-clamp recording from outside-out patches, we measured the concentration dependence of response amplitude and steady-state desensitization, the single-channel conductance and the maximum open probability. The GluA4 subunit seems critical for the functional properties of AMPA receptors in AII amacrines and immunocytochemical labeling suggested that GluA4 is located at synapses made by both OFF-cone bipolar cells and rod bipolar cells. Finally, we used a series of experimental observables to develop kinetic models for AII amacrine AMPA receptors and subsequently used the models to explore the behavior of the receptors and responses generated by glutamate concentration profiles mimicking those occurring in synapses. These models will facilitate future in silico modeling of synaptic signaling and processing in AII amacrine cells.
URI
http://hdl.handle.net/1956/17158
DOI
10.1007/s00429-017-1520-1
Citation
Hartveit E, Zandt B, Madsen E, Castilho A, Mørkve SH, Veruki ML. AMPA receptors at ribbon synapses in the mammalian retina: kinetic models and molecular identity. Brain Structure and Function. 2017
Publisher
Springer
Subject
Amacrine cellsGlutamate receptorsKinetic schemePatch clampRetina
Collections
  • Department of Biomedicine 322
Copyright Springer-Verlag GmbH Germany 2017

University of Bergen Library
Contact Us | Send Feedback
 

 

Browse

All of BORACommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypeThis CollectionBy Issue DateAuthorsTitlesSubjectsType

My Account

LoginRegister

Statistics

View Usage Statistics

University of Bergen Library
Contact Us | Send Feedback