dc.contributor.author | Harasimczuk, Ewa | |
dc.contributor.author | Øvergård, Aina-Cathrine | |
dc.contributor.author | Grotmol, Sindre | |
dc.contributor.author | Nilsen, Frank | |
dc.contributor.author | Dalvin, Sussie | |
dc.date.accessioned | 2018-04-16T13:00:18Z | |
dc.date.available | 2018-04-16T13:00:18Z | |
dc.date.issued | 2018-01 | |
dc.Published | Harasimczuk E, Øvergård A, Grotmol SG, Nilsen F, Dalvin ST. Characterization of three salmon louse (Lepeophtheirus salmonis) genes with fibronectin II domains expressed by tegumental type 1 glands. Molecular and biochemical parasitology. 2018;219:1-9 | eng |
dc.identifier.issn | 1872-9428 | en_US |
dc.identifier.issn | 0166-6851 | en_US |
dc.identifier.uri | https://hdl.handle.net/1956/17618 | |
dc.description.abstract | The salmon louse, Lepeophtheirus salmonis (Copepoda: Caligidae), is currently the most significant pathogen affecting the salmon farming industry in the Northern Hemisphere. Exocrine glands of blood-feeding parasites are believed to be important for the host-parasite interaction, but also in the production of substances for integument lubrication and antifouling. In L. salmonis; however, we have limited knowledge about the exocrine glands. The aim of this study was therefore to examine three genes containing fibronectin type II (FNII) domains expressed in L. salmonis tegumental type 1 (teg 1) glands, namely LsFNII1, 2 and 3. LsFNII1, 2 and 3 contains four, three, and two FNII domains respectively. Sequence alignment of LsFNII domains showed conservation of amino acids that may indicate a possible involvement of LsFNII domains in collagen binding. Ontogenetic analysis of LsFNII1, 2 and 3 revealed highest expression in pre-adult and adult lice. Localization of LsFNII1, 2 and 3 transcripts showed expression in teg 1 glands only, which are the most abundant exocrine gland type in L. salmonis. LsFNII1, 2 and 3 were successfully knocked-down by RNAi, however, alteration in gland morphology was not detected between the knock-down and control groups. Overall, this study gives first insight into FNII domain containing proteins in L. salmonis. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Elsevier | en_US |
dc.rights | Attribution CC BY-NC-ND | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | eng |
dc.subject | Crustacea | eng |
dc.subject | Exocrine glands | eng |
dc.subject | In situ hybridization | eng |
dc.subject | Integument | eng |
dc.subject | RNA interference | eng |
dc.subject | Sea lice | eng |
dc.title | Characterization of three salmon louse (Lepeophtheirus salmonis) genes with fibronectin II domains expressed by tegumental type 1 glands | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2018-01-31T14:48:40Z | |
dc.description.version | acceptedVersion | en_US |
dc.rights.holder | Copyright 2017 The Author(s) | en_US |
dc.identifier.doi | https://doi.org/10.1016/j.molbiopara.2017.12.002 | |
dc.identifier.cristin | 1527302 | |
dc.source.journal | Molecular and biochemical parasitology (Print) | |
dc.relation.project | Norges forskningsråd: 203513 | |