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dc.contributor.authorHarasimczuk, Ewa
dc.contributor.authorØvergård, Aina-Cathrine
dc.contributor.authorGrotmol, Sindre
dc.contributor.authorNilsen, Frank
dc.contributor.authorDalvin, Sussie
dc.date.accessioned2018-04-16T13:00:18Z
dc.date.available2018-04-16T13:00:18Z
dc.date.issued2018-01
dc.identifier.citationHarasimczuk E, Øvergård A, Grotmol SG, Nilsen F, Dalvin ST. Characterization of three salmon louse (Lepeophtheirus salmonis) genes with fibronectin II domains expressed by tegumental type 1 glands. Molecular and biochemical parasitology. 2018;219:1-9eng
dc.identifier.urihttp://hdl.handle.net/1956/17618
dc.description.abstractThe salmon louse, Lepeophtheirus salmonis (Copepoda: Caligidae), is currently the most significant pathogen affecting the salmon farming industry in the Northern Hemisphere. Exocrine glands of blood-feeding parasites are believed to be important for the host-parasite interaction, but also in the production of substances for integument lubrication and antifouling. In L. salmonis; however, we have limited knowledge about the exocrine glands. The aim of this study was therefore to examine three genes containing fibronectin type II (FNII) domains expressed in L. salmonis tegumental type 1 (teg 1) glands, namely LsFNII1, 2 and 3. LsFNII1, 2 and 3 contains four, three, and two FNII domains respectively. Sequence alignment of LsFNII domains showed conservation of amino acids that may indicate a possible involvement of LsFNII domains in collagen binding. Ontogenetic analysis of LsFNII1, 2 and 3 revealed highest expression in pre-adult and adult lice. Localization of LsFNII1, 2 and 3 transcripts showed expression in teg 1 glands only, which are the most abundant exocrine gland type in L. salmonis. LsFNII1, 2 and 3 were successfully knocked-down by RNAi, however, alteration in gland morphology was not detected between the knock-down and control groups. Overall, this study gives first insight into FNII domain containing proteins in L. salmonis.eng
dc.language.isoengeng
dc.publisherElseviereng
dc.rightsAttribution CC BY-NC-NDeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/eng
dc.subjectCrustaceaeng
dc.subjectExocrine glandseng
dc.subjectIn situ hybridizationeng
dc.subjectIntegumenteng
dc.subjectRNA interferenceeng
dc.subjectSea liceeng
dc.titleCharacterization of three salmon louse (Lepeophtheirus salmonis) genes with fibronectin II domains expressed by tegumental type 1 glandseng
dc.typeJournal articleeng
dc.date.updated2018-01-31T14:48:40Z
dc.rights.holderCopyright 2017 The Author(s)eng
dc.type.versionacceptedVersioneng
bora.peerreviewedPeer reviewedeng
dc.type.documentJournal article
dc.identifier.cristinID1527302
dc.identifier.doi10.1016/j.molbiopara.2017.12.002eng
dc.source.issn0166-6851eng
dc.source.issn1872-9428eng
dc.relation.projectIDNorges forskningsråd: 203513
dc.relation.journalMolecular and biochemical parasitology (Print)


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