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dc.contributor.authorHarasimczuk, Ewa
dc.contributor.authorØvergård, Aina-Cathrine
dc.contributor.authorGrotmol, Sindre
dc.contributor.authorNilsen, Frank
dc.contributor.authorDalvin, Sussie
dc.identifier.citationHarasimczuk E, Øvergård A, Grotmol SG, Nilsen F, Dalvin ST. Characterization of three salmon louse (Lepeophtheirus salmonis) genes with fibronectin II domains expressed by tegumental type 1 glands. Molecular and biochemical parasitology. 2018;219:1-9eng
dc.description.abstractThe salmon louse, Lepeophtheirus salmonis (Copepoda: Caligidae), is currently the most significant pathogen affecting the salmon farming industry in the Northern Hemisphere. Exocrine glands of blood-feeding parasites are believed to be important for the host-parasite interaction, but also in the production of substances for integument lubrication and antifouling. In L. salmonis; however, we have limited knowledge about the exocrine glands. The aim of this study was therefore to examine three genes containing fibronectin type II (FNII) domains expressed in L. salmonis tegumental type 1 (teg 1) glands, namely LsFNII1, 2 and 3. LsFNII1, 2 and 3 contains four, three, and two FNII domains respectively. Sequence alignment of LsFNII domains showed conservation of amino acids that may indicate a possible involvement of LsFNII domains in collagen binding. Ontogenetic analysis of LsFNII1, 2 and 3 revealed highest expression in pre-adult and adult lice. Localization of LsFNII1, 2 and 3 transcripts showed expression in teg 1 glands only, which are the most abundant exocrine gland type in L. salmonis. LsFNII1, 2 and 3 were successfully knocked-down by RNAi, however, alteration in gland morphology was not detected between the knock-down and control groups. Overall, this study gives first insight into FNII domain containing proteins in L. salmonis.eng
dc.rightsAttribution CC BY-NC-NDeng
dc.subjectExocrine glandseng
dc.subjectIn situ hybridizationeng
dc.subjectRNA interferenceeng
dc.subjectSea liceeng
dc.titleCharacterization of three salmon louse (Lepeophtheirus salmonis) genes with fibronectin II domains expressed by tegumental type 1 glandseng
dc.typeJournal articleeng
dc.rights.holderCopyright 2017 The Author(s)eng
bora.peerreviewedPeer reviewedeng
dc.type.documentJournal article
dc.relation.projectIDNorges forskningsråd: 203513
dc.relation.journalMolecular and biochemical parasitology (Print)

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