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dc.contributor.authorLeal, Gracianoen_US
dc.contributor.authorComprido, Diogoen_US
dc.contributor.authorde Luca, Pasqualinoen_US
dc.contributor.authorMorais, Eduardoen_US
dc.contributor.authorRodrigues, Luísen_US
dc.contributor.authorMele, Mirandaen_US
dc.contributor.authorSantos, Ana R.en_US
dc.contributor.authorCosta, Rui O.en_US
dc.contributor.authorPinto, Maria Joanaen_US
dc.contributor.authorPatil, Sudarshanen_US
dc.contributor.authorBerentsen, Birgitteen_US
dc.contributor.authorAfonso, Pedroen_US
dc.contributor.authorCarreto, Lauraen_US
dc.contributor.authorLi, Ka Wanen_US
dc.contributor.authorPinheiro, Pauloen_US
dc.contributor.authorAlmeida, Ramiro D.en_US
dc.contributor.authorSantos, Manuel A. S.en_US
dc.contributor.authorBramham, Clive R.en_US
dc.contributor.authorDuarte, Carlos B.en_US
dc.date.accessioned2018-06-13T12:15:14Z
dc.date.available2018-06-13T12:15:14Z
dc.date.issued2017-11
dc.PublishedLeal, Comprido, de Luca, Morais, Rodrigues L, Mele, Santos A, Costa R, Pinto, Patil S, Berentsen B, Afonso P, Carreto, Li, Pinheiro, Almeida, Santos, Bramham CRE, Duarte. The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neurons. eNeuro. 2017;4(6):e0268-17.2017eng
dc.identifier.issn2373-2822
dc.identifier.urihttps://hdl.handle.net/1956/17794
dc.description.abstractBrain-derived neurotrophic factor (BDNF) is an important mediator of long-term synaptic potentiation (LTP) in the hippocampus. The local effects of BDNF depend on the activation of translation activity, which requires the delivery of transcripts to the synapse. In this work, we found that neuronal activity regulates the dendritic localization of the RNA-binding protein heterogeneous nuclear ribonucleoprotein K (hnRNP K) in cultured rat hippocampal neurons by stimulating BDNF-Trk signaling. Microarray experiments identified a large number of transcripts that are coimmunoprecipitated with hnRNP K, and about 60% of these transcripts are dissociated from the protein upon stimulation of rat hippocampal neurons with BDNF. In vivo studies also showed a role for TrkB signaling in the dissociation of transcripts from hnRNP K upon high-frequency stimulation (HFS) of medial perforant path-granule cell synapses of male rat dentate gyrus (DG). Furthermore, treatment of rat hippocampal synaptoneurosomes with BDNF decreased the coimmunoprecipitation of hnRNP K with mRNAs coding for glutamate receptor subunits, Ca2+- and calmodulin-dependent protein kinase IIβ (CaMKIIβ) and BDNF. Downregulation of hnRNP K impaired the BDNF-induced enhancement of NMDA receptor (NMDAR)-mediated mEPSC, and similar results were obtained upon inhibition of protein synthesis with cycloheximide. The results demonstrate that BDNF regulates specific populations of hnRNP-associated mRNAs in neuronal dendrites and suggests an important role of hnRNP K in BDNF-dependent forms of synaptic plasticity.en_US
dc.language.isoengeng
dc.publisherSociety for Neuroscienceeng
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/4.0eng
dc.subjectBDNFeng
dc.subjectlocal translationeng
dc.subjectlong-term synaptic potentiationeng
dc.subjectneurotrophinseng
dc.subjectNMDA receptorseng
dc.subjectRNA transporteng
dc.titleThe RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neuronsen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2018-01-18T11:44:36Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2017 The Author(s)
dc.identifier.doihttps://doi.org/10.1523/eneuro.0268-17.2017
dc.identifier.cristin1546272
dc.source.journaleNeuro
dc.relation.projectNorges forskningsråd: 204861
dc.relation.projectNorges forskningsråd: 199355


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