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dc.contributor.authorFeng, Haiyang
dc.contributor.authorThompson, Eric
dc.date.accessioned2018-12-05T09:24:14Z
dc.date.available2018-12-05T09:24:14Z
dc.date.issued2018
dc.identifier.urihttp://hdl.handle.net/1956/18742
dc.description.abstractOogenesis in the urochordate, Oikopleura dioica, occurs in a large coenocyst in which vitellogenesis precedes oocyte selection in order to adapt oocyte production to nutrient conditions. The animal has expanded Cyclin-Dependant Kinase 1 (CDK1) and Cyclin B paralog complements, with several expressed during oogenesis. Here, we addressed functional redundancy and specialization of CDK1 and cyclin B paralogs during oogenesis and early embryogenesis through spatiotemporal analyses and knockdown assays. CDK1a translocated from organizing centres (OCs) to selected meiotic nuclei at the beginning of the P4 phase of oogenesis, and its knockdown impaired vitellogenesis, nurse nuclear dumping, and entry of nurse nuclei into apoptosis. CDK1d-Cyclin Ba translocated from OCs to selected meiotic nuclei in P4, drove meiosis resumption and promoted nuclear envelope breakdown (NEBD). CDK1d-Cyclin Ba was also involved in histone H3S28 phosphorylation on centromeres and meiotic spindle assembly through regulating Aurora B localization to centromeres during prometaphase I. In other studied species, Cyclin B3 commonly promotes anaphase entry, but we found O. dioica Cyclin B3a to be non-essential for anaphase entry during oogenic meiosis. Instead, Cyclin B3a contributed to meiotic spindle assembly though its loss could be compensated by Cyclin Ba.eng
dc.language.isoengeng
dc.publisherTaylor & Franciseng
dc.rightsAttribution CC BY-NC-NDeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/eng
dc.subjectUrochordateeng
dc.subjectchromosomal passenger complexeng
dc.subjectvitellogenesiseng
dc.subjectcyclin B3eng
dc.subjectoogenesiseng
dc.subjectmeiosis resumptioneng
dc.titleSpecialization of CDK1 and cyclin B paralog functions in a coenocystic mode of oogenic meiosiseng
dc.typeJournal articleeng
dc.rights.holderCopyright 2018 The Author(s)eng
dc.type.versionpublishedVersioneng
bora.peerreviewedPeer reviewedeng
bora.journalTitleCell Cycleeng
bibo.volume17eng
bibo.issue12eng
bibo.pageStart1425eng
bibo.pageEnd1444eng
dc.identifier.cristinID1622393
dc.identifier.doi10.1080/15384101.2018.1486167eng
dc.source.issn1538-4101eng


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Except where otherwise noted, this item's license is described as Attribution CC BY-NC-ND