dc.contributor.author | Strauss, Philipp | en_US |
dc.contributor.author | Marti, Hans-Peter | en_US |
dc.contributor.author | Beisland, Christian | en_US |
dc.contributor.author | Scherer, Andreas | en_US |
dc.contributor.author | Lysne, Vegard | en_US |
dc.contributor.author | Leh, Sabine Maria | en_US |
dc.contributor.author | Flatberg, Arnar | en_US |
dc.contributor.author | Koch, Even Evjen | en_US |
dc.contributor.author | Beisvag, Vidar | en_US |
dc.contributor.author | Landolt, Lea Zoe | en_US |
dc.contributor.author | Skogstrand, Trude | en_US |
dc.contributor.author | Eikrem, Øystein Solberg | en_US |
dc.date.accessioned | 2019-05-28T09:19:50Z | |
dc.date.available | 2019-05-28T09:19:50Z | |
dc.date.issued | 2018-03-10 | |
dc.Published | Strauss P, Marti HP, Beisland C, Scherer A, Lysne V, Leh S, Flatberg A, Koch EE, Beisvag V, Landolt LZ, Skogstrand T, Eikrem ØS. Expanding the utilization of formalin-fixed, paraffin-embedded archives: Feasibility of miR-Seq for disease exploration and biomarker development from biopsies with clear cell renal cell Carcinoma. International Journal of Molecular Sciences. 2018;19(3):803 | eng |
dc.identifier.issn | 1422-0067 | |
dc.identifier.uri | https://hdl.handle.net/1956/19759 | |
dc.description.abstract | Novel predictive tools for clear cell renal cell carcinoma (ccRCC) are urgently needed. MicroRNAs (miRNAs) have been increasingly investigated for their predictive value, and formalin-fixed paraffin-embedded biopsy archives may potentially be a valuable source of miRNA sequencing material, as they remain an underused resource. Core biopsies of both cancerous and adjacent normal tissues were obtained from patients (n = 12) undergoing nephrectomy. After small RNA-seq, several analyses were performed, including classifier evaluation, obesity-related inquiries, survival analysis using publicly available datasets, comparisons to the current literature and ingenuity pathway analyses. In a comparison of tumour vs. normal, 182 miRNAs were found with significant differential expression; miR-155 was of particular interest as it classified all ccRCC samples correctly and correlated well with tumour size (R2 = 0.83); miR-155 also predicted poor survival with hazard ratios of 2.58 and 1.81 in two different TCGA (The Cancer Genome Atlas) datasets in a univariate model. However, in a multivariate Cox regression analysis including age, sex, cancer stage and histological grade, miR-155 was not a statistically significant survival predictor. In conclusion, formalin-fixed paraffin-embedded biopsy tissues are a viable source of miRNA-sequencing material. Our results further support a role for miR-155 as a promising cancer classifier and potentially as a therapeutic target in ccRCC that merits further investigation. | en_US |
dc.language.iso | eng | eng |
dc.publisher | MDPI | eng |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0 | eng |
dc.subject | microRNA/miRNA | eng |
dc.subject | miR-155 | eng |
dc.subject | clear cell renal cell carcinoma/ccRCC | eng |
dc.subject | formalin-fixed paraffin-embedded/FFPE | eng |
dc.subject | next generation sequencing/NGS | eng |
dc.title | Expanding the utilization of formalin-fixed, paraffin-embedded archives: Feasibility of miR-Seq for disease exploration and biomarker development from biopsies with clear cell renal cell Carcinoma | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2019-01-16T13:36:58Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2018 The Author(s) | |
dc.source.articlenumber | 803 | |
dc.identifier.doi | https://doi.org/10.3390/ijms19030803 | |
dc.identifier.cristin | 1590269 | |
dc.source.journal | International Journal of Molecular Sciences | |
dc.identifier.citation | International Journal of Molecular Sciences. 2018;19(3):803 | |
dc.source.volume | 19 | |
dc.source.issue | 3 | |