dc.contributor.author | Turcu, Diana Cornelia | |
dc.contributor.author | Lillehaug, Johan R. | |
dc.contributor.author | Seo, Hee-Chan | |
dc.date.accessioned | 2020-05-03T16:07:47Z | |
dc.date.available | 2020-05-03T16:07:47Z | |
dc.date.issued | 2019-12 | |
dc.Published | Turcu D, Lillehaug JR, Seo H. SIX3 and SIX6 interact with GEMININ via C-terminal regions. Biochemistry and Biophysics Reports. 2019;20 | eng |
dc.identifier.issn | 2405-5808 | en_US |
dc.identifier.uri | http://hdl.handle.net/1956/22068 | |
dc.description.abstract | The histoarchitecture and function of eye and forebrain depend on a well-controlled balance between cell proliferation and differentiation. For example, the binding of the cell cycle regulator GEMININ to CDT1, which is a part of the pre-replication complex, promotes cell differentiation. Homeodomain transcription factors SIX3 and SIX6 also interact with GEMININ of which SIX3-GEMININ interaction promotes cell proliferation, whereas the nature of SIX6-GEMININ interaction has not been studied to date. We investigated SIX3/SIX6 and GEMININ interactions using bimolecular fluorescence complementation, surface plasmon resonance and isothermal titration calorimetry. Interactions between SIX3/SIX6 and GEMININ were detected in mammalian cells in culture. The presence of the C-terminal regions of SIX3 and SIX6 proteins, but not their SIX domains or homeodomains as previously thought, were required for interaction with GEMININ. Interestingly, the disordered C- and N- terminal regions of GEMININ were involved in binding to SIX3/SIX6. The coiled-coil region of GEMININ, which is the known protein-binding domain and also interacts with CDT1, was not involved in GEMININ-SIX3/SIX6 interaction. Using SPR and ITC, SIX3 bound GEMININ with a micromolar affinity and the binding stoichiometry was 1:2 (SIX3 - GEMININ). The present study gives new insights into the binding properties of SIX proteins, especially the role of their variable and disordered C-terminal regions. | en_US |
dc.language.iso | eng | eng |
dc.publisher | Elsevier | en_US |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | eng |
dc.subject | SIX3 | eng |
dc.subject | SIX6 | eng |
dc.subject | GEMININCell-cycle regulation | eng |
dc.subject | Bimolecular fluorescence complementation (BiFC) | eng |
dc.subject | Surface plasmon resonance (SPR) | eng |
dc.subject | Isothermal titration calorimetry (ITC) | eng |
dc.subject | Protein interaction | eng |
dc.title | SIX3 and SIX6 interact with GEMININ via C-terminal regions | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2020-01-10T13:25:08Z | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2019 The Authors | en_US |
dc.identifier.doi | https://doi.org/10.1016/j.bbrep.2019.100695 | |
dc.identifier.cristin | 1764561 | |
dc.source.journal | Biochemistry and Biophysics Reports | |