|dc.description.abstract||During the last decade resin-based dental restorative materials have replaced amalgam as the
first choice dental filling material. Resin-based dental restorative materials are complex
polymers containing a variety of monomers and filler particles, as well as initiators,
activators, stabilizers, plasticizers and other additives. Several studies have shown that many
of the ingredients are leaching from the materials, even after adequate polymerization. It is
known from in vitro studies that some of the compounds in the resin-based materials have
cytotoxic, genotoxic or estrogenic potential. Allergenic effects in patients and dental
personnel have also been reported.
The aim of this study was to identify and quantify substances released from various types of
resin-based dental restorative materials. Specimens were polymerized according to protocols
from the producers and submerged in different solvents. To characterize a maximum elution
potential, ethanol was used as an immersion media. Furthermore, immersion in Ringer’s
solution and saliva was used to mimic clinical elution conditions.
A combined Gas Chromatography - Mass Spectrometry (GC-MS) technique was used for
the identification and quantification of eluates. The majority of eluting substances from the
polymeric matrix are organic substances with low molecular weight, which are well suited
for analysis by GC-MS. Tailor-made internal standards for HEMA and TEGDMA were
synthesized for the quantification procedure.
We have identified and quantified a number of compounds from several materials.
Significant differences regarding type and amount of leachables between the materials are