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dc.contributor.authorKikuta, Hiroshieng
dc.contributor.authorLaplante, Maryeng
dc.contributor.authorNavrátilová, Pavlaeng
dc.contributor.authorKomisarczuk, Anna Zofiaeng
dc.contributor.authorEngström, Pär G.eng
dc.contributor.authorFredman, Davideng
dc.contributor.authorAkalin, Altunaeng
dc.contributor.authorCaccamo, Marioeng
dc.contributor.authorSealy, Ianeng
dc.contributor.authorHowe, Kerstineng
dc.contributor.authorGhislain, Julieneng
dc.contributor.authorPezeron, Guillaumeeng
dc.contributor.authorMourrain, Philippeeng
dc.contributor.authorEllingsen, Staaleeng
dc.contributor.authorOates, Andrew C.eng
dc.contributor.authorThisse, Christineeng
dc.contributor.authorThisse, Bernardeng
dc.contributor.authorFoucher, Isabelleeng
dc.contributor.authorAdolf, Birgiteng
dc.contributor.authorGeling, Andreaeng
dc.contributor.authorLenhard, Boriseng
dc.contributor.authorBecker, Thomas S.eng
dc.date.accessioned2008-07-29T12:32:04Z
dc.date.available2008-07-29T12:32:04Z
dc.date.issued2007-03-06eng
dc.PublishedGenome Research 17: 545-555en
dc.identifier.issn1088-9051
dc.identifier.urihttps://hdl.handle.net/1956/2742
dc.description.abstractWe report evidence for a mechanism for the maintenance of long-range conserved synteny across vertebrate genomes. We found the largest mammal-teleost conserved chromosomal segments to be spanned by highly conserved noncoding elements (HCNEs), their developmental regulatory target genes, and phylogenetically and functionally unrelated "bystander" genes. Bystander genes are not specifically under the control of the regulatory elements that drive the target genes and are expressed in patterns that are different from those of the target genes. Reporter insertions distal to zebrafish developmental regulatory genes pax6.1/2, rx3, id1, and fgf8 and miRNA genes mirn9-1 and mirn9-5 recapitulate the expression patterns of these genes even if located inside or beyond bystander genes, suggesting that the regulatory domain of a developmental regulatory gene can extend into and beyond adjacent transcriptional units. We termed these chromosomal segments genomic regulatory blocks (GRBs). After whole genome duplication in teleosts, GRBs, including HCNEs and target genes, were often maintained in both copies, while bystander genes were typically lost from one GRB, strongly suggesting that evolutionary pressure acts to keep the single-copy GRBs of higher vertebrates intact. We show that loss of bystander genes and other mutational events suffered by duplicated GRBs in teleost genomes permits target gene identification and HCNE/target gene assignment. These findings explain the absence of evolutionary breakpoints from large vertebrate chromosomal segments and will aid in the recognition of position effect mutations within human GRBs.en_US
dc.language.isoengeng
dc.publisherCold Spring Harbor Laboratory Presseng
dc.titleGenomic regulatory blocks encompass multiple neighboring genes and maintain conserved synteny in vertebrateseng
dc.typePeer reviewedeng
dc.typeJournal articleeng
dc.identifier.doihttps://doi.org/10.1101/gr.6086307


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