Herbal plants used in traditional Chinese medicine as a source of compounds with potential medical utilities - identification, characterization and organization
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The history and knowledge of Chinese herbal medicine is over 3000 years old, and over 10 000 herbs are recorded and are in use for different conditions. This is an enormous amount of knowledge that can be used to find new lead compounds for Western medicine. The genus Abies, of the Pinaceae family, has been extensively investigated at MRCTCM. Different Abies species have been used in folk medicine, and it has been reported bioactivities from crude extracts of several species. Abies forrestii is one of 14 species that are native to P.R. China, and little research has been done on this specific plant. In this master thesis, eight compounds were obtained from the aerial parts of Abies forrestii, by use of several different chromatographic techniques (size exclusion, TLC, normal and reverse phase). The chemical structure was determined for three of them by use of MS- and NMR-techniques (the known diterpenes abietic acid, dehydroabietic acid, 9,13(β)-epidioxy-8(14)-abieten-18-oic acid) and for one of them by comparing to a standard on TLC (β-sitosterol). The rest was either not pure enough or was obtained in a too low concentration. The pulverized plant material was extracted with ethanol, purified with ethylacetate and fractionated with gradient elution over a silica gel column and a MCI-column. The compounds were isolated and purified over silica gel-, Sephadex LH-20- and ODS-columns, and with P-TLC. The structures of the compounds were determined by a combination of LC-ESI MS, 1H- and 13C NMR data. The extracted compounds in this thesis will be included in MRCTCM’s Natural Products Library and registered in their MDL ISIS Database. Abies and Abies forrestii is of continuing interest at MRCTCM. The Natural Products Library and the MDL ISIS Database are, and will become an even larger resource for discovering new medicines and for understanding how Chinese herbal treatment work.
This Master thesis has been written at the Gade Institute and the Center of Pharmacy, University of Bergen, Norway.The study presented in this thesis was carried out as part of an international staff exchange programme titled “Traditional Chinese Medicine in the Post-genomic Era: Identifying Lead Therapeutic Compounds Against Cancer”. The European Council is acknowledged for the support of this program by FP7-PEOPLE-IRSES-2008, Marie Curies Actions – International Research Staff Exchange Scheme 2009 – 2013, project number 230232. The practical part of the thesis was carried out at the Modern Research Centre of Traditional Chinese Medicine at the Second Military Medical University in Shanghai from August 2009 to December 2009. This master thesis is part of the project Traditional Chinese Medicine in the Post-Genomic Era: Identifying Lead Therapeutic Compounds Against Cancer (TCMCANCER). It is a collaboration project between Centre de Recherche Public de la Santé in Luxembourg, Institute of Medicinal Plant Development in Beijing, China, Modern Research Center of Traditional Chinese Medicine, Second Military Medical University in Shanghai, China and the Gade Institute, University of Bergen in Norway.
PublisherThe University of Bergen
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