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dc.contributor.authorDankel, Simon N.eng
dc.contributor.authorFadnes, Dag J.eng
dc.contributor.authorStavrum, Anne-Kristineng
dc.contributor.authorStansberg, Christineeng
dc.contributor.authorHoldhus, Ritaeng
dc.contributor.authorHoang, Tuyen Thi Vaneng
dc.contributor.authorVeum, Vivian L.eng
dc.contributor.authorChristensen, Bjørn Josteineng
dc.contributor.authorVåge, Villyeng
dc.contributor.authorSagen, Jørn V.eng
dc.contributor.authorSteen, Vidar Martineng
dc.contributor.authorMellgren, Gunnareng
dc.identifier.citationPLoS ONE 5(6): e11033en
dc.description.abstractBackground In obesity, impaired adipose tissue function may promote secondary disease through ectopic lipid accumulation and excess release of adipokines, resulting in systemic low-grade inflammation, insulin resistance and organ dysfunction. However, several of the genes regulating adipose tissue function in obesity are yet to be identified. Methodology/Principal Findings In order to identify novel candidate genes that may regulate adipose tissue function, we analyzed global gene expression in abdominal subcutaneous adipose tissue before and one year after bariatric surgery (biliopancreatic diversion with duodenal switch, BPD/DS) (n = 16). Adipose tissue from lean healthy individuals was also analyzed (n = 13). Two different microarray platforms (AB 1700 and Illumina) were used to measure the differential gene expression, and the results were further validated by qPCR. Surgery reduced BMI from 53.3 to 33.1 kg/m2. The majority of differentially expressed genes were down-regulated after profound fat loss, including transcription factors involved in stress response, inflammation, and immune cell function (e.g., FOS, JUN, ETS, C/EBPB, C/EBPD). Interestingly, a distinct set of genes was up-regulated after fat loss, including homeobox transcription factors (IRX3, IRX5, HOXA5, HOXA9, HOXB5, HOXC6, EMX2, PRRX1) and extracellular matrix structural proteins (COL1A1, COL1A2, COL3A1, COL5A1, COL6A3). Conclusions/Significance The data demonstrate a marked switch of transcription factors in adipose tissue after profound fat loss, providing new molecular insight into a dichotomy between stress response and metabolically favorable tissue development. Our findings implicate homeobox transcription factors as important regulators of adipose tissue function.en
dc.publisherPublic Library of Scienceeng
dc.rightsAttribution CC BYeng
dc.titleSwitch from stress response to homeobox transcription factors in Adipose tissue after profound fat losseng
dc.typePeer reviewedeng
dc.typeJournal articleeng
dc.subject.nsiVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750nob
dc.subject.nsiVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714nob
dc.rights.holderCopyright 2010 Dankel et al.
dc.rights.holderDankel et al.eng
bora.peerreviewedPeer reviewedeng

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