Vis enkel innførsel

dc.contributor.authorCarvalho, Rejane Hugheseng
dc.contributor.authorHaberle, Vanjaeng
dc.contributor.authorHou, Juneng
dc.contributor.authorvan Gent, Teuseng
dc.contributor.authorThongjuea, Supateng
dc.contributor.authorvan Ijcken, Wilfred F.J.eng
dc.contributor.authorKockx, Christeleng
dc.contributor.authorBrouwer, Rutgereng
dc.contributor.authorRijkers, Erikjaneng
dc.contributor.authorSieuwerts, Anietaeng
dc.contributor.authorFoekens, Johneng
dc.contributor.authorvan Vroonhoven, Mirjameng
dc.contributor.authorAerts, Joachimeng
dc.contributor.authorGrosveld, Frankeng
dc.contributor.authorLenhard, Boriseng
dc.contributor.authorPhilipsen, Sjaakeng
dc.date.accessioned2014-11-06T10:51:20Z
dc.date.available2014-11-06T10:51:20Z
dc.date.issued2012-06-22eng
dc.identifier.issn1756-8935en_US
dc.identifier.urihttps://hdl.handle.net/1956/8714
dc.description.abstractBackground: Non-small cell lung carcinoma (NSCLC) is a complex malignancy that owing to its heterogeneity and poor prognosis poses many challenges to diagnosis, prognosis and patient treatment. DNA methylation is an important mechanism of epigenetic regulation involved in normal development and cancer. It is a very stable and specific modification and therefore in principle a very suitable marker for epigenetic phenotyping of tumors. Here we present a genome-wide DNA methylation analysis of NSCLC samples and paired lung tissues, where we combine MethylCap and next generation sequencing (MethylCap-seq) to provide comprehensive DNA methylation maps of the tumor and paired lung samples. The MethylCap-seq data were validated by bisulfite sequencing and methyl-specific polymerase chain reaction of selected regions. Results: Analysis of the MethylCap-seq data revealed a strong positive correlation between replicate experiments and between paired tumor/lung samples. We identified 57 differentially methylated regions (DMRs) present in all NSCLC tumors analyzed by MethylCap-seq. While hypomethylated DMRs did not correlate to any particular functional category of genes, the hypermethylated DMRs were strongly associated with genes encoding transcriptional regulators. Furthermore, subtelomeric regions and satellite repeats were hypomethylated in the NSCLC samples. We also identified DMRs that were specific to two of the major subtypes of NSCLC, adenocarcinomas and squamous cell carcinomas. Conclusions: Collectively, we provide a resource containing genome-wide DNA methylation maps of NSCLC and their paired lung tissues, and comprehensive lists of known and novel DMRs and associated genes in NSCLC.en_US
dc.language.isoengeng
dc.publisherBioMed Centralen_US
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/2.0eng
dc.subjectDNA methylationeng
dc.subjectEpigeneticseng
dc.subjectMethylCapeng
dc.subjectNext generation sequencingeng
dc.subjectNon-small cell lung Cancereng
dc.titleGenome-wide DNA methylation profiling of non-small cell lung carcinomasen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2013-08-23T09:15:10Z
dc.description.versionPeer Reviewed
dc.description.versionpublishedVersionen_US
dc.rights.holderRejane Carvalho et al.; licensee BioMed Central Ltd.en_US
dc.rights.holderCopyright 2012 Hughes Carvalho et al.; licensee BioMed Central Ltden_US
dc.source.articlenumber9
dc.identifier.doihttps://doi.org/10.1186/1756-8935-5-9
dc.identifier.cristin959965
dc.source.journalEpigenetics & Chromatin
dc.source.405


Tilhørende fil(er)

Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail
Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Attribution CC BY
Med mindre annet er angitt, så er denne innførselen lisensiert som Attribution CC BY