Blar i Department of Biomedicine på forfatter "Aksnes, Henriette"
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Co-translational, post-translational, and non-catalytic roles of N-terminal acetyltransferases
Aksnes, Henriette; Ree, Rasmus Moen; Arnesen, Thomas (Peer reviewed; Journal article, 2019)Recent studies of N-terminal acetylation have identified new N-terminal acetyltransferases (NATs) and expanded the known functions of these enzymes beyond their roles as ribosome-associated co-translational modifiers. For ... -
Efficient and crucial quality control of HAP1 cell ploidy status
Beigl, Tobias B.; Kjosås, Ine; Seljeseth, Emilie; Glomnes, Nina; Aksnes, Henriette (Journal article; Peer reviewed, 2020)The near-haploid human cell line HAP1 recently became a popular subject for CRISPR/Cas9 editing, since only one allele requires modification. Through the gene-editing service at Horizon Discovery, there are at present more ... -
Expanded in vivo substrate profile of the yeast N-terminal acetyltransferase NatC
Van Damme, Petra; Osberg, Camilla; Jonckheere, Veronique; Glomnes, Nina; Gevaert, Kris; Arnesen, Thomas; Aksnes, Henriette (Journal article; Peer reviewed, 2022)N-terminal acetylation is a conserved protein modification among eukaryotes. The yeast Saccharomyces cerevisiae is a valuable model system for studying this modification. The bulk of protein N-terminal acetylation in S. ... -
Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays
Hellesvik, Monica; Øye, Hanne; Aksnes, Henriette (Journal article; Peer reviewed, 2020)3D cell culture assays are becoming increasingly popular due to their higher resemblance to tissue environment. These provide an increased complexity compared to the growth on 2D surface and therefore allow studies of ... -
Loss of N-terminal acetyltransferase A activity induces thermally unstable ribosomal proteins and increases their turnover in Saccharomyces cerevisiae
Guzman, Ulises H.; Aksnes, Henriette; Ree, Rasmus; Krogh, Nicolai; Jakobsson, Magnus; Jensen, Lars J.; Arnesen, Thomas; Olsen, Jesper V. (Journal article; Peer reviewed, 2023)Protein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. Cells with defective Nt-acetylation display a wide array of phenotypes ... -
N-terminal acetylation of actin by NAA80 is essential for structural integrity of the Golgi apparatus
Beigl, Tobias; Hellesvik, Monica; Saraste, Jaakko; Arnesen, Thomas; Aksnes, Henriette (Journal article; Peer reviewed, 2020)N-alpha-acetyltransferase 80 (NAA80) was recently demonstrated to acetylate the N-terminus of actin, with NAA80 knockout cells showing actin cytoskeleton-related phenotypes, such as increased formation of membrane protrusions ...