Blar i Department of Biomedicine på forfatter "Wiig, Helge"
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Abnormal neonatal sodium handling in skin precedes hypertension in the SAME rat
Mullins, Linda; Ivy, Jessica; Ward, Mairi; Tenstad, Olav; Wiig, Helge; Kitada, Kento; Manning, Jon; Rakova, Natalia; Müller, Dominik; Mullins, John (Journal article; Peer reviewed, 2021)We discovered high Na+ and water content in the skin of newborn Sprague–Dawley rats, which reduced ~ 2.5-fold by 7 days of age, indicating rapid changes in extracellular volume (ECV). Equivalent changes in ECV post birth ... -
Dendritic cells loaded with tumor antigens and a dual immunostimulatory and anti-IL-10 specific siRNA can prime T lymphocytes against leukemic cells
Iversen, Per Ole; Semaeva, Elvira; Sørensen, Dag R.; Wiig, Helge; Sioud, Mouldy (Peer reviewed; Journal article, 2009-12)Vaccines using dendritic cells (DCs) harboring leukemic antigens to stimulate T cells is a possible treatment of acute myeloid leukemia (AML). Limitations of breaking tolerance to leukemic cells and lack of specific ... -
Effect of Topical Anaesthetics on Interstitial Colloid Osmotic Pressure in Human Subcutaneous Tissue Sampled by Wick Technique
Guthe, Hans Jørgen Timm; Nedrebø, Torbjørn; Tenstad, Olav; Wiig, Helge; Berg, Ansgar (Peer reviewed; Journal article, 2012-02-14)Objectives: To measure colloid osmotic pressure in interstitial fluid (COPi) from human subcutaneous tissue with the modified wick technique in order to determine influence of topical application of anaesthetics, dry vs. ... -
Fluid transport from the dental pulp revisited.
Berggreen, Ellen; Wiig, Helge; Virtej, Anca (Journal article; Peer reviewed, 2020-10)In the dental pulp surrounded by rigid dentinal walls, an increase in fluid volume will be followed by a rapid increase in interstitial fluid pressure. To maintain pressure homeostasis, a fluid drainage system is required. ... -
High-salt diet causes osmotic gradients and hyperosmolality in skin without affecting interstitial fluid and lymph
Nikpey Esfahlan, Elham; Karlsen, Tine Veronica; Rakova, Natalia; Titze, Jens M.; Tenstad, Olav; Wiig, Helge (Peer reviewed; Journal article, 2017-04)The common notion is that the body Na+ is maintained within narrow limits for fluid and blood pressure homeostasis. Several studies have, however, shown that considerable amounts of Na+ can be retained or removed from the ... -
Increased plasma colloid osmotic pressure facilitates the uptake of therapeutic macromolecules in a xenograft tumour model
Hofmann, Matthias; McCormack, Emmet; Mujic, Maja; Roßberg, Maila; Bernd, August; Bereiter-Hahn, Jürgen; Gjertsen, Bjørn Tore; Wiig, Helge; Kippenberger, Stefan (Peer reviewed; Journal article, 2009-08)Elevated tumor interstitial fluid pressure (TIFP) is a characteristic of most solid tumors. Clinically, TIFP may hamper the uptake of chemotherapeutic drugs into the tumor tissue reducing their therapeutic efficacy. In ... -
Interstitial fluid drainage from rat apical area takes place via vessels in the mandibular canal.
Virtej, Anca; Tenstad, Olav; Wiig, Helge; Berggreen, Ellen (Journal article; Peer reviewed, 2020)We sought to investigate the transport route for protein‐rich fluid from the apical area towards the draining lymph nodes. The first mandibular molar root canals in 24 female Wistar rats were instrumented and filled with ... -
Interstitial fluid: the overlooked component of the tumor microenvironment?
Wiig, Helge; Tenstad, Olav; Iversen, Per Ole; Kalluri, Raghu; Bjerkvig, Rolf (Peer reviewed; Journal article, 2010-07-23)Background: The interstitium, situated between the blood and lymph vessels and the cells, consists of a solid or matrix phase and a fluid phase, together constituting the tissue microenvironment. Here we focus on the ... -
Interstitial IgG antibody pharmacokinetics assessed by combined in vivo- and physiologically-based pharmacokinetic modelling approaches
Eigenmann, Miro Julian; Karlsen, Tine Veronica; Krippendorff, Ben-Fillippo; Tenstad, Olav; Fronton, Ludivine; Otteneder, Michael B.; Wiig, Helge (Peer reviewed; Journal article, 2017-12)For most therapeutic antibodies, the interstitium is the target space. Although experimental methods for measuring antibody pharmacokinetics (PK) in this space are not well established, thus making quantitative assessment ... -
The interstitium conducts extrarenal storage of sodium and represents a third compartment essential for extracellular volume and blood pressure homeostasis
Wiig, Helge; Luft, FC; Titze, Jens M. (Peer reviewed; Journal article, 2018-03)The role of salt in the pathogenesis of arterial hypertension is not well understood. According to the current understanding, the central mechanism for blood pressure (BP) regulation relies on classical studies linking BP ... -
Lipopolysaccharide induces filtrate leakage from renal tubular lumina into the interstitial space via a proximal tubular Toll-like receptor 4–dependent pathway and limits sensitivity to fluid therapy in mice
Nakano, Daisuke; Kitada, Kento; Wan, Ningning; Zhang, Yahua; Wiig, Helge; Wararat, Kittikulsuth; Yanagita, Motoko; Lee, Syann; Jia, Lin; Titze, Jens M.; Nishiyama, Akira (Journal article; Peer reviewed, 2019-12-16)Sustained oliguria during fluid resuscitation represents a perplexing problem in patients undergoing therapy for septic acute kidney injury. Here, we tested whether lipopolysaccharide induces filtrate leakage from the ... -
Lowering of tumor interstitial fluid pressure reduces tumor cell proliferation in a xenograft tumor model
Hofmann, Matthias; Guschel, Maike; Bernd, August; Bereiter-Hahn, Jürgen; Kaufmann, Robert; Tandi, Christa; Wiig, Helge; Kippenberger, Stefan (Peer reviewed; Journal article, 2006-02)High tumor interstitial fluid pressure (TIFP) is a characteristic of most solid tumors. TIFP may hamper adequate uptake of macromolecular therapeutics in tumor tissue. In addition, TIFP generates mechanical forces affecting ... -
Na+ is shifted from the extracellular to the intracellular compartment and is not inactivated by glycosaminoglycans during high salt conditions in rats
Thowsen, Irene Matre; Karlsen, Tine Veronica; Nikpey, Elham; Haslene-Hox, Hanne; Skogstrand, Trude; Randolph, Gwendalyn J.; Zinselmeyer, Bernd H.; Tenstad, Olav; Wiig, Helge (Journal article; Peer reviewed, 2022)Recently, studies have emerged suggesting that the skin plays a role as major Na+ reservoir via regulation of the content of glycosaminoglycans and osmotic gradients. We investigated whether there were electrolyte gradients ... -
A New Method for Isolation of Interstitial Fluid from Human Solid Tumors Applied to Proteomic Analysis of Ovarian Carcinoma Tissue
Haslene-Hox, Hanne; Oveland, Eystein; Berg, Kaja Christine Graue; Kolmannskog, Odd; Woie, Kathrine; Salvesen, Helga Birgitte; Tenstad, Olav; Wiig, Helge (Peer reviewed; Journal article, 2011-04-26)Major efforts have been invested in the identification of cancer biomarkers in plasma, but the extraordinary dynamic range in protein composition, and the dilution of disease specific proteins make discovery in plasma ... -
Pharmacokinetics and pharmacodynamics of t-cell bispecifics in the tumour interstitial fluid
Eigenmann, Miro Julian; Karlsen, Tine Veronica; Wagner, Marek; Tenstad, Olav; Weinzierl, Tina; Fauti, Tanja; Grimm, Hans Peter; Skogstrand, Trude; Klein, Christian; Sam, Johannes; Umana, Pablo; Bacac, Marina; Wiig, Helge; Walz, Antje-Christine (Journal article; Peer reviewed, 2021)The goal of this study is to investigate the pharmacokinetics in plasma and tumour interstitial fluid of two T-cell bispecifics (TCBs) with different binding affinities to the tumour target and to assess the subsequent ... -
Quantification of the concentration gradient of biomarkers between ovarian carcinoma interstitial fluid and blood
Haslene-Hox, Hanne; Madani, Amina; Berg, Kaja Christine Graue; Woie, Kathrine; Salvesen, Helga Birgitte; Wiig, Helge; Tenstad, Olav (Peer reviewed; Journal article, 2014-12)Background: Tumor interstitial fluid (TIF) rather than plasma should be used in cancer biomarker discovery because of the anticipated higher concentration of locally produced proteins in the tumor microenvironment. ... -
Tumor interstitial fluid formation, characterization, and clinical implications
Wagner, Marek; Wiig, Helge (Peer reviewed; Journal article, 2015-05-26)The interstitium, situated between the blood and lymph vessels and the cells, consists of a solid or matrix phase and a fluid phase representing the tissue microenvironment. In the present review, we focus on the interstitial ... -
VEGF-B hypertrophy predisposes to transition from diastolic to systolic heart failure in hypertensive rats
Samuelsson, Anne-Maj; Bartolomaeus, Theda Ulrike Patricia; Anandakumar, Harithaa; Thowsen, Irene Matre; Nikpey, Elham; Han, Jianhua; Marko, Lajos; Finne, Kenneth; Tenstad, Olav; Eckstein, Johannes; Berndt, Nikolaus; Kühne, Titus; Kedziora, Sarah; Sultan, Ibrahim; Skogstrand, Trude; Karlsen, Tine Veronica; Nurmi, Harri; Forslund, Sofia K.; Bollano, Entela; Alitalo, Kari; Muller, Dominik N.; Wiig, Helge (Journal article; Peer reviewed, 2023)Aims: Cardiac energy metabolism is centrally involved in heart failure (HF), although the direction of the metabolic alterations is complex and likely dependent on the particular stage of HF progression. Vascular endothelial ...