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dc.contributor.authorPeris, David
dc.contributor.authorJanssen, Kathrin
dc.contributor.authorBarthel, H. Jonas
dc.contributor.authorBierbaum, Gabriele
dc.contributor.authorDelclòs, Xavier
dc.contributor.authorPeñalver, Enrique
dc.contributor.authorSolórzano-Kraemer, Mónica M.
dc.contributor.authorJordal, Bjarte Henry
dc.contributor.authorRust, Jes
dc.date.accessioned2021-05-07T12:55:51Z
dc.date.available2021-05-07T12:55:51Z
dc.date.created2020-12-14T22:35:27Z
dc.date.issued2020
dc.PublishedPLOS ONE. 2020, 15 (9), .
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/11250/2754199
dc.description.abstractPast claims have been made for fossil DNA recovery from various organisms (bacteria, plants, insects and mammals, including humans) dating back in time from thousands to several million years BP. However, many of these recoveries, especially those described from million-year-old amber (fossil resin), have faced criticism as being the result of modern environmental contamination and for lack of reproducibility. Using modern genomic techniques, DNA can be obtained with confidence from a variety of substrates (e.g. bones, teeth, gum, museum specimens and fossil insects) of different ages, albeit always less than one million years BP, and results can also be obtained from much older materials using palaeoproteomics. Nevertheless, new attempts to determine if ancient DNA (aDNA) is present in insects preserved in 40 000-year old sub-fossilised resin, the precursor of amber, have been unsuccessful or not well documented. Resin-embedded specimens are therefore regarded as unsuitable for genetic studies. However, we demonstrate here, for the first time, that although a labile molecule, DNA is still present in platypodine beetles (Coleoptera: Curculionidae) embedded in six-year-old and two-year-old resin pieces from Hymenaea verrucosa (Angiospermae: Fabaceae) collected in Madagascar. We describe an optimised method which meets all the requirements and precautions for aDNA experiments for our purpose: to explore the DNA preservation limits in resin. Our objective is far from starting an uncontrolled search for aDNA in amber as it was in the past, but to start resolving basic aspects from the DNA preservation in resin and search from the most modern samples to the ancient ones, step by step. We conclude that it is therefore possible to study genomics from resin-embedded organisms, although the time limits remain to be determined.en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleDNA from resin-embedded organisms: Past, present and futureen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2020 Peris et al.en_US
dc.source.articlenumbere0239521en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.1371/journal.pone.0239521
dc.identifier.cristin1859779
dc.source.journalPLOS ONEen_US
dc.source.4015
dc.source.149
dc.identifier.citationPLOS ONE. 2020, 15 (9), e0239521.en_US
dc.source.volume15en_US
dc.source.issue9en_US


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal