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dc.contributor.authorVelde, Susanne Grønnestad
dc.date.accessioned2021-12-16T07:28:25Z
dc.date.issued2021-12-03
dc.date.submitted2021-12-15T23:00:10Z
dc.identifier.urihttps://hdl.handle.net/11250/2834599
dc.description.abstractThe development and function of cells in the nervous system and the musculature require regulated interactions with the extracellular matrix. The dystrophin-glycoprotein complex is an important regulator of these interactions. A central component of this complex is dystroglycan, a membrane protein that is heavily glycosylated on its extracellular part. This glycosylation is initiated by O-mannosyltransferases and is then expanded by a series of additional glycosyltransferases. The sea anemone Nematostella vectensis represents an evolutionary ancient group of animals and has emerged as a powerful tool to study neurogenesis and development in an evolutionary context. The genome of Nematostella encodes all members of the dystrophin-glycoprotein complex, introducing the question of their role in this animal. In this study, we show that the expression pattern of dystroglycan and five glycosyltransferases are compatible with different roles of dystroglycan O- mannosylation. By in-situ hybridization, we identified POMGNT1, POMT1 and POMT2 to display a similar expression pattern throughout early development. Double fluorescence in- situ hybridization shows the enzymes to be co-expressed with each other, suggesting a shared function. Difference in early expression between the glycosyltransferases and dystroglycan might indicate a dystroglycan-independent function of O-mannosylation, whereas the similar expression in later stages indicate a potential role for dystroglycan-dependent O-mannosylation during Nematostella development. Furthermore, double fluorescence in-situ hybridization found POMGNT1 to be expressed in FoxQ2d-expressing sensory cells, suggesting the enzyme to have a role in sensory cells. Finally, CRISPR/Cas9 was successfully used to introduce a mutation in the gene of POMGNT1, to further study the function of this glycosyltransferase and its substrate in Nematostella development. We anticipate this study to be a starting point for future studies to provide insight on the role of the dystrophin- glycoprotein complex and the necessity of alpha-dystroglycan O-mannosylation on the nervous and muscular systems of Nematostella vectensis.
dc.language.isoeng
dc.publisherThe University of Bergen
dc.rightsCopyright the Author. All rights reserved
dc.subjectNematostella
dc.subjectdevelopment
dc.subjectPOMGNT1
dc.subjectneurogenesis
dc.subjectdystrophin-glycoprotein complex
dc.subjectdystroglycan
dc.titleThe dystrophin-glycoprotein complex in the development of Nematostella vectensis: Expression analyses and generation of a mutant by genome editing
dc.typeMaster thesis
dc.date.updated2021-12-15T23:00:10Z
dc.rights.holderCopyright the Author. All rights reserved
dc.description.degreeMasteroppgave i molekylærbiologi
dc.description.localcodeMOL399
dc.description.localcodeMAMN-MOL
dc.subject.nus759929
fs.subjectcodeMOL399
fs.unitcode12-60-0


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