dc.contributor.author | Labun, Kornel | |
dc.contributor.author | Krause, Maximilian | |
dc.contributor.author | Torres Cleuren, Yamila Nicole | |
dc.contributor.author | Valen, Eivind Dale | |
dc.date.accessioned | 2022-01-31T13:59:40Z | |
dc.date.available | 2022-01-31T13:59:40Z | |
dc.date.created | 2022-01-06T11:59:24Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 1934-3396 | |
dc.identifier.uri | https://hdl.handle.net/11250/2976066 | |
dc.description.abstract | The design of optimal guide RNA (gRNA) sequences for CRISPR systems is challenged by the need to achieve highly efficient editing at the desired location (on-target editing) with minimal editing at unintended locations (off-target editing). Although laboratory validation should ideally be used to detect off-target activity, computational predictions are almost always preferred in practice due to their speed and low cost. Several studies have therefore explored gRNA-DNA interactions in order to understand how CRISPR complexes select their genomic targets. CHOPCHOP (https://chopchop.cbu.uib.no/) leverages these developments to build a user-friendly web interface that helps users design optimal gRNAs. CHOPCHOP supports a wide range of CRISPR applications, including gene knock-out, sequence knock-in, and RNA knock-down. Furthermore, CHOPCHOP offers visualization that enables an informed choice of gRNAs and supports experimental validation. In these protocols, we describe the best practices for gRNA design using CHOPCHOP. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley | en_US |
dc.relation.uri | https://currentprotocols.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cpz1.46 | |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | CRISPR Genome Editing Made Easy Through the CHOPCHOP Website | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2021 The Author(s) | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.1002/cpz1.46 | |
dc.identifier.cristin | 1975827 | |
dc.source.journal | Current Protocols in Bioinformatics | en_US |
dc.relation.project | Norges forskningsråd: 250049 | en_US |
dc.relation.project | Kreftforeningen: 190290 | en_US |
dc.identifier.citation | Current Protocols in Bioinformatics. 2021 | en_US |