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dc.contributor.authorKummer, Daniel
dc.contributor.authorSteinbacher, Tim
dc.contributor.authorThölmann, Sonja
dc.contributor.authorSchwietzer, Mariel Flavia
dc.contributor.authorHartmann, Christian
dc.contributor.authorHorenkamp, Simone
dc.contributor.authorDemuth, Sabrina
dc.contributor.authorPeddibhotla, Swetha S.D.
dc.contributor.authorBrinkmann, Frauke
dc.contributor.authorKemper, Björn
dc.contributor.authorSchnekenburger, Jürgen
dc.contributor.authorBrandt, Matthias
dc.contributor.authorBetz, Timo
dc.contributor.authorLiashkovich, Ivan
dc.contributor.authorKouzel, Ivan
dc.contributor.authorShahin, Victor
dc.contributor.authorCorvaia, Nathalie
dc.contributor.authorRottner, Klemens
dc.contributor.authorTarbashevich, Katsiaryna
dc.contributor.authorRaz, Erez
dc.contributor.authorGreune, Lilo
dc.contributor.authorAlexander Schmidt, Schmidt
dc.contributor.authorGerke, Volker
dc.contributor.authorEbnet, Klaus
dc.date.accessioned2023-03-31T09:04:11Z
dc.date.available2023-03-31T09:04:11Z
dc.date.created2022-06-11T12:44:36Z
dc.date.issued2022
dc.identifier.issn0021-9525
dc.identifier.urihttps://hdl.handle.net/11250/3061353
dc.description.abstractContact inhibition of locomotion (CIL) is a process that regulates cell motility upon collision with other cells. Improper regulation of CIL has been implicated in cancer cell dissemination. Here, we identify the cell adhesion molecule JAM-A as a central regulator of CIL in tumor cells. JAM-A is part of a multimolecular signaling complex in which tetraspanins CD9 and CD81 link JAM-A to αvβ5 integrin. JAM-A binds Csk and inhibits the activity of αvβ5 integrin-associated Src. Loss of JAM-A results in increased activities of downstream effectors of Src, including Erk1/2, Abi1, and paxillin, as well as increased activity of Rac1 at cell–cell contact sites. As a consequence, JAM-A-depleted cells show increased motility, have a higher cell–matrix turnover, and fail to halt migration when colliding with other cells. We also find that proper regulation of CIL depends on αvβ5 integrin engagement. Our findings identify a molecular mechanism that regulates CIL in tumor cells and have implications on tumor cell dissemination.en_US
dc.language.isoengen_US
dc.publisherRockefeller University Pressen_US
dc.rightsNavngivelse-Ikkekommersiell-DelPåSammeVilkår 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/deed.no*
dc.titleAJAM-A–tetraspanin–αvβ5 integrin complex regulates contact inhibition of locomotionen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.source.articlenumbere202105147en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2
dc.identifier.doi10.1083/jcb.202105147
dc.identifier.cristin2030996
dc.source.journalJournal of Cell Biologyen_US
dc.identifier.citationJournal of Cell Biology. 2022, 221 (4), e202105147.en_US
dc.source.volume221en_US
dc.source.issue4en_US


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Navngivelse-Ikkekommersiell-DelPåSammeVilkår 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse-Ikkekommersiell-DelPåSammeVilkår 4.0 Internasjonal