Functional characterization of all-trans retinoic acid-induced differentiation factor (ATRAID)
dc.contributor.author | Mehrasa, Roya | |
dc.contributor.author | Cristea, Ileana | |
dc.contributor.author | Bredrup, Cecilie | |
dc.contributor.author | Rødahl, Eyvind | |
dc.contributor.author | Bruland, Ove | |
dc.date.accessioned | 2023-09-27T11:26:48Z | |
dc.date.available | 2023-09-27T11:26:48Z | |
dc.date.created | 2023-09-20T11:30:35Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 2211-5463 | |
dc.identifier.uri | https://hdl.handle.net/11250/3092372 | |
dc.description.abstract | All-trans retinoic acid-induced differentiation (ATRAID) factor was first identified in HL60 cells. Several mRNA isoforms exist, but the respective proteins have not been fully characterized. In transfected cells expressing Myc-Flag-tagged ATRAID Isoform (Iso) A, B, and C, Iso C was found to be expressed at high levels, Iso A was found to be expressed at low levels due to rapid degradation, and the predicted protein expressed from Iso B was not detected. Iso C was present mainly in an N-glycosylated form. In subcellular fractionation experiments, Iso C localized to the membranous and nuclear fractions, while immunofluorescence analysis revealed that Iso C is located close to the plasma membrane, mainly in cytoplasmic vesicles and in the Golgi area. We confirm that Iso C colocalizes to some extent with endosomal/lysosomal markers LAMP1 and LAMP2. Furthermore, we show that ATRAID co-localizes with RAB11, a GTPase associated with recycling endosomes and implicated in regulating vesicular trafficking. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.title | Functional characterization of all-trans retinoic acid-induced differentiation factor (ATRAID) | en_US |
dc.type | Journal article | en_US |
dc.type | Peer reviewed | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Copyright 2023 The Author(s) | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | 10.1002/2211-5463.13685 | |
dc.identifier.cristin | 2176958 | |
dc.source.journal | FEBS Open Bio | en_US |
dc.identifier.citation | FEBS Open Bio. 2023. | en_US |
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