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dc.contributor.authorBrenner, Annette
dc.contributor.authorFrøystein, Nils Åge
dc.date.accessioned2019-06-21T12:56:11Z
dc.date.available2019-06-21T12:56:11Z
dc.date.issued2014-06-26
dc.PublishedBrenner AK, Frøystein NÅ. Using MUSIC and CC(CO)NH for Backbone Assignment of Two Medium-Sized Proteins Not Fully Accessible to Standard 3D NMR. Molecules. 2014;19(7):8890-8903eng
dc.identifier.issn1420-3049en_US
dc.identifier.urihttps://hdl.handle.net/1956/20342
dc.description.abstractThe backbone assignment of medium-sized proteins is rarely as straightforward as that of small proteins, and thus often requires creative solutions. Here, we describe the application of a combination of standard 3D heteronuclear methods with CC(CO)NH and a variety of MUltiplicity Selective In-phase Coherence transfer (MUSIC) experiments. Both CC(CO)NH and MUSIC are, in theory, very powerful methods for the backbone assignment of proteins. Due to low sensitivity, their use has usually been linked to small proteins only. However, we found that combining CC(CO)NH and MUSIC experiments simplified the assignment of two challenging medium-sized proteins of 13 and 19.5 kDa, respectively. These methods are to some extent complementary to each other: CC(CO)NH acquired with a long isotropic mixing time can identify amino acids with large aliphatic side chains. Whereas the most sensitive MUSIC experiments identify amino acid types that cannot be detected by CC(CO)NH, comprising the residues with acid and amide groups, and aromatic rings in their side chains. Together these methods provide a means of identifying the majority of peaks in the 2D 15N HSQC spectrum which simplifies the backbone assignment work even for proteins, e.g., small kinases, whose standard spectra resulted in little spectral resolution and low signal intensities.en_US
dc.language.isoengeng
dc.publisherMDPIen_US
dc.rightsAttribution CC BYeng
dc.rights.urihttp://creativecommons.org/licenses/by/3.0eng
dc.subjectprotein NMR spectroscopyeng
dc.subjectbackbone assignmenteng
dc.subjectCC(CO)NHeng
dc.subjectMUSICeng
dc.titleUsing MUSIC and CC(CO)NH for Backbone Assignment of Two Medium-Sized Proteins Not Fully Accessible to Standard 3D NMRen_US
dc.typePeer reviewed
dc.typeJournal article
dc.date.updated2019-01-24T10:43:57Z
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2014 The Author(s)en_US
dc.identifier.doihttps://doi.org/10.3390/molecules19078890
dc.identifier.cristin1143278
dc.source.journalMolecules


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