dc.contributor.author | Furnes, Clemens | eng |
dc.contributor.author | Arnesen, Thomas | eng |
dc.contributor.author | Askjaer, Peter | eng |
dc.contributor.author | Kjems, Jørgen | eng |
dc.contributor.author | Szilvay, Anne Marie | eng |
dc.date.accessioned | 2014-09-12T07:58:21Z | |
dc.date.available | 2014-09-12T07:58:21Z | |
dc.date.issued | 2005-06-10 | eng |
dc.identifier.issn | 1742-4690 | en_US |
dc.identifier.uri | https://hdl.handle.net/1956/8458 | |
dc.description.abstract | Background: The HIV-1 Rev regulatory protein binds as an oligomeric complex to viral RNA mediating nuclear export of incompletely spliced and non-spliced viral mRNAs encoding the viral structural proteins. However, the biological significance of the obligatory complex formation of Rev upon the viral RNA is unclear. Results: The activity of various fusion proteins based on the negative oligomerization-defect Rev mutant M4 was tested using Rev dependent reporter constructs. An artificial M4 mutant dimer and an M4 mutant containing an extra basic domain from the HTLV-I Rex protein exhibited nearly full activity when compared to wild type Rev. Conclusion: Rev dimerization appears to be required to expose free basic domains whilst the Rev oligomeric complex remains bound to viral RNA via other basic domains. | en_US |
dc.language.iso | eng | eng |
dc.publisher | BioMed Central | en_US |
dc.rights | Attribution CC BY | eng |
dc.rights.uri | http://creativecommons.org/licenses/by/2.0 | eng |
dc.title | HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain | en_US |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.date.updated | 2013-08-28T17:17:45Z | |
dc.description.version | Peer Reviewed | |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | Clemens Furnes et al.; licensee BioMed Central Ltd. | en_US |
dc.rights.holder | Copyright 2005 Furnes et al; licensee BioMed Central Ltd. | en_US |
dc.source.articlenumber | 39 | |
dc.identifier.doi | https://doi.org/10.1186/1742-4690-2-39 | |
dc.identifier.cristin | 396733 | |
dc.source.journal | Retrovirology | |
dc.source.40 | 2 | |