Blar i Bergen Open Research Archive på forfatter "Varland, Sylvia"

Viser treff 1-6 av 6

    • Actin Post-translational Modifications: The Cinderella of Cytoskeletal Control 

      Varland, Sylvia; Vandekerckhove, Joel; Drazic, Adrian (Peer reviewed; Journal article, 2019)
      Actin is one of the most abundant proteins in eukaryotic cells and the main component of the microfilament system. It plays essential roles in numerous cellular activities including muscle contraction, maintenance of cell ...

    • The Final Maturation State of β-actin Involves N-terminal Acetylation by NAA80, not N-terminal Arginylation by ATE1 

      Drazic, Adrian; Timmerman, Evy; Kajan, Ulrike; Marie, Michaël Bruno Eric; Varland, Sylvia; Impens, Francis; Gevaert, Kris; Arnesen, Thomas (Journal article; Peer reviewed, 2022)
      Actin is a hallmark protein of the cytoskeleton in eukaryotic cells, affecting a range of cellular functions. Actin dynamics is regulated through a myriad of actin-binding proteins and post-translational modifications. The ...

    • Human NAA30 can rescue yeast mak3∆ mutant growth phenotypes 

      Drazic, Adrian; Varland, Sylvia (Journal article; Peer reviewed, 2021)
      N-terminal acetylation is an irreversible protein modification that primarily occurs co-translationally, and is catalyzed by a highly conserved family of N-terminal acetyltransferases (NATs). The NatC complex (NAA30–NAA35–NAA38) ...

    • N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity 

      Varland, Sylvia; Silva, Rui Duarte; Kjosås, Ine; Faustino, Alexandra; Bogaert, Annelies; Billmann, Maximilian; Boukhatmi, Hadi; Kellen, Barbara; Costanzo, Michael; Drazic, Adrian; Osberg, Camilla; Chan, Katherine; Zhang, Xiang; Tong, Amy Hin Yan; Andreazza, Simonetta; Lee, Juliette J.; Nedyalkova, Lyudmila; Ušaj, Matej; Whitworth, Alexander J.; Andrews, Brenda J.; Moffat, Jason; Myers, Chad L.; Gevaert, Kris; Boone, Charles; Martinho, Rui Gonçalo; Arnesen, Thomas (Journal article; Peer reviewed, 2023)
      Most eukaryotic proteins are N-terminally acetylated, but the functional impact on a global scale has remained obscure. Using genome-wide CRISPR knockout screens in human cells, we reveal a strong genetic dependency between ...

    • N-terminal modifications of cellular proteins: the enzymes involved, their substrate specificities and biological effects 

      Varland, Sylvia; Osberg, Camilla; Arnesen, Thomas (Peer reviewed; Journal article, 2015-06-16)
      The vast majority of eukaryotic proteins are N-terminally modified by one or more processing enzymes. Enzymes acting on the very first amino acid of a polypeptide include different peptidases, transferases, and ligases. ...

    • PFN2 and NAA80 cooperate to efficiently acetylate the N-terminus of actin 

      Reed, Rasmus; Kind, Laura; Kaziales, Anna; Varland, Sylvia; Dai, Minglu; Richter, Klaus; Drazic, Adrian; Arnesen, Thomas (Journal article; Peer reviewed, 2020)
      The actin cytoskeleton is of profound importance to cell shape, division, and intracellular force generation. Profilins bind to globular (G-)actin and regulate actin filament formation. Although profilins are well-established ...