Blar i Bergen Open Research Archive på forfatter "Øvrebø, Kjell Kåre"
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Bacteria and fungi in acute cholecystitis. A prospective study comparing next generation sequencing to culture
Dyrhovden, Ruben; Øvrebø, Kjell Kåre; Nordahl, Magnus Vie; Nygaard, Randi M.; Ulvestad, Elling; Kommedal, Øyvind (Peer reviewed; Journal article, 2020)Objectives: Guidelines for antibiotic treatment of acute cholecystitis are based on studies using culture techniques for microbial identification. Microbial culture has well described limitations and more comprehensive ... -
Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers
Dyrhovden, Ruben; Rippin, Martin; Øvrebø, Kjell Kåre; Nygaard, Randi Monsen; Ulvestad, Elling; Kommedal, Øyvind (Journal article; Peer reviewed, 2021)In this article, we investigate patterns of microbial DNA contamination in targeted 16S rRNA amplicon sequencing (16S deep sequencing) and demonstrate how this can be used to filter background bacterial DNA in diagnostic ...