Blar i Bergen Open Research Archive på forfatter "Appel, Silke"
-
Aberrant signaling of immune cells in Sjögren's syndrome patient subgroups upon interferon stimulation
Sarkar, Irene; Davies, Richard Allan; Aarebrot, Anders Krogh; Solberg, Silje Michelsen; Petrovic, Aleksandra; Joshi, Anagha; Bergum, Brith; Brun, Johan Gorgas; Hammenfors, Daniel; Jonsson, Roland; Appel, Silke (Journal article; Peer reviewed, 2022)Background: Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease, characterized by mononuclear cell infiltrates in the salivary and lacrimal glands, leading to glandular atrophy and dryness. Patient heterogeneity ... -
Altered phenotype and Stat1 expression in Toll-like receptor 7/8 stimulated monocyte-derived dendritic cells from patients with primary Sjögren's syndrome
Vogelsang, Petra; Karlsen, Marie; Brun, Johan Gorgas; Jonsson, Roland; Appel, Silke (Peer reviewed; Journal article, 2014-08-11)Introduction: Dendritic cells (DC) are the most potent antigen-presenting cells of the immune system, involved in both initiating immune responses and maintaining tolerance. Dysfunctional and via toll-like receptor (TLR) ... -
The Bacterial Preparation OK432 Induces IL-12p70 Secretion in Human Dendritic Cells in a TLR3 Dependent Manner
Hovden, Arnt-Ove; Karlsen, Marie; Jonsson, Roland; Appel, Silke (Peer reviewed; Journal article, 2012-02-21)Dendritic cells (DC) used in therapeutic cancer immunotherapy have to be able to stimulate T cells resulting in an immune response that can efficiently target the cancer cells. One of the critical hurdles has been the lack ... -
Complement C4 Copy Number Variation is Linked to SSA/Ro and SSB/La Autoantibodies in Systemic Inflammatory Autoimmune Diseases
Lundtoft, Christian; Pucholt, Pascal; Martin, Myriam; Bianchi, Matteo; Lundström, Emeli; Eloranta, Maija-Leena; Sandling, Johanna K.; Sjöwall, Christopher; Jönsen, Andreas; Gunnarsson, Iva; Rantapää-Dahlqvist, Solbritt; Bengtsson, Anders A.; Leonard, Dag; Baecklund, Eva; Jonsson, Roland; Hammenfors, Daniel; Forsblad-d'Elia, Helena; Eriksson, Per; Mandl, Thomas; Magnusson Bucher, Sara; Norheim, Katrine Brække; Johnsen, Svein Joar Auglæn; Omdal, Roald; Kvarnström, Marika; Wahren Herlenius, Marie Elisabeth; Notarnicola, Antonella; Andersson, Anna Helena; Molberg, Øyvind; Diederichsen, Louise Pyndt; Almlöf, Jonas; Syvänen, Ann-Christine; Kozyrev, Sergey V.; Lindblad-Toh, Kerstin; Rönnblom, Lars; Brokstad, Karl Albert; Skarstein, Kathrine; Jonsson, Malin Viktoria; Appel, Silke; Aqrawi, Lara A.; Jensen, Janicke Liaaen; Palm, Øyvind; Nilsson, Birgitta Blakstad; Blom, Anna M.; Lundberg, Ingrid E.; Nordmark, Gunnel; Diaz-Gallo, Lina Marcela; Svenungsson, Elisabet; Rönnblom, Lars (Journal article; Peer reviewed, 2022)Objective Copy number variation of the C4 complement components, C4A and C4B, has been associated with systemic inflammatory autoimmune diseases. This study was undertaken to investigate whether C4 copy number variation ... -
The Culture Dish Surface Influences the Phenotype and Cytokine Production of Human Monocyte-Derived Dendritic Cells
Sauter, Alexander; Yi, Dag Heiro; Li, Yayan; Roersma, Sabine; Appel, Silke (Peer reviewed; Journal article, 2019-10-02)Monocyte-derived dendritic cells (moDC) are an important scientific and clinical source of functional dendritic cells (DC). However, the optimization of the generation process has to date mainly been limited to the variation ... -
Dendritic cell populations in patients with self-reported food hypersensitivity
Lied, Gülen Arslan; Vogelsang, Petra; Berstad, Arnold; Appel, Silke (Peer reviewed; Journal article, 2011-05)Self-reported hypersensitivity to food is a common condition and many of these patients have indications of intestinal immune activation. Dendritic cells (DCs) are recognized as the most potent antigen-presenting cells ... -
Dendritic cell populations in patients with self-reported food hypersensitivity
Lied, Gülen Arslan; Vogelsang, Petra; Berstad, Arnold; Appel, Silke (Conference poster, 2010) -
Effective polyethylene glycol passivation for the inhibition of surface interactions of peripheral blood mononuclear cells and platelets
Sauter, Alexander; Richter, Gunther; Micoulet, Alexandre; Martinez, Aurora; Spatz, Joachim P.; Appel, Silke (Peer reviewed; Journal article, 2013-06-20)The inhibition of unspecific adhesion of human white blood cells is a prerequisite for applications requiring the control of defined surface interactions. In this study, a passivation agent based on polyethylene glycol ... -
Evaluation of β-catenin inhibition of axitinib and nitazoxanide in human monocyte-derived dendritic cells
Azeem, Waqas; Bakke, Ragnhild Maukon; Gabriel, Benjamin; Appel, Silke; Øyan, Anne Margrete; Kalland, Karl-Henning (Journal article; Peer reviewed, 2021)Modulation of β-catenin signaling has attractive therapeutic potential in cancer immunotherapy. Several studies have found that β-catenin can mediate immune evasion in cancer and promote anti-inflammatory features of ... -
Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells
Sprater, Florian; Hovden, Arnt-Ove; Appel, Silke (Peer reviewed; Journal article, 2012-11-19)Dendritic cells (DC) are the only hematopoietic cells expressing the epithelial specific Ets transcription factor ESE-3. Here we analyzed presence and quantity of isoforms ESE-3a, ESE-3b and ESE-3j in various immunogenic ... -
Expression of toll-like receptor -7 and -9 in B cell subsets from patients with primary Sjögren's syndrome
Karlsen, Marie; Hansen, Torbjørn; Nordal, Hilde Haugedal; Brun, Johan G; Jonsson, Roland; Appel, Silke (Peer reviewed; Journal article, 2015-03-19)Introduction: Sjögren’s syndrome (SS) is a rheumatic autoimmune disease characterized by inflammation of exocrine glands. As autoantibodies are present in a majority of patients, B cells have been suggested to play an ... -
Herring roe oil in treatment of psoriasis – influence on immune cells and cytokine network
Petrovic, Aleksandra; Bueide, Ingvild Øye; Tveit, Kåre Steinar; Hallaråker, Hogne; Bjørndal, Bodil; Holmes, Timothy Dunstan; Davies, Richard Allan; Brokstad, Karl Albert; Bergum, Brith; Appel, Silke (Journal article; Peer reviewed, 2023)Background: Psoriasis is a chronic immune-mediated skin disease with systemic inflammation and comorbidities. Although the disease severity may vary over time, many patients suffer from mild to moderate disease. Often local ... -
Impaired activation of STAT5 upon IL-2 stimulation in Tregs and elevated sIL-2R in Sjögren’s syndrome
Keindl, Magdalena; Davies, Richard Allan; Bergum, Brith; Brun, Johan Gorgas; Hammenfors, Daniel; Jonsson, Roland; Lyssenko, Valeriya; Appel, Silke (Journal article; Peer reviewed, 2022)Background Interleukin-2 (IL-2) and the high-affinity IL-2 receptor (IL-2R) are essential for the survival of regulatory T cells (Tregs) which are the main players in immune tolerance and prevention of autoimmune diseases. ... -
In vitro suppression of immune responses using monocyte-derived tolerogenic dendritic cells from patients with primary Sjögren's syndrome
Volchenkov, Roman; Brun, Johan Gorgas; Jonsson, Roland; Appel, Silke (Peer reviewed; Journal article, 2013-09-09)Introduction: Therapeutic vaccination with antigen-specific tolerogenic dendritic cells (tolDC) might become a future option of individualized therapy for patients with autoimmune diseases. In this study, we tested the ... -
Increased Plasma Soluble Interleukin-2 Receptor Alpha Levels in Patients With Long-Term Type 1 Diabetes With Vascular Complications Associated With IL2RA and PTPN2 Gene Polymorphisms
Keindl, Magdalena; Fedotkina, Olena; du Plessis, Elsa; Jain, Ruchi; Bergum, Brith; Mygind Jensen, Troels; Laustrup Møller, Cathrine; Falhammar, Henrik; Nyström, Thomas; Catrina, Sergiu-Bogdan; Jörneskog, Gun; Groop, Leif; Eliasson, Mats; Eliasson, Björn; Brismar, Kerstin; Nilsson, Peter M.; Berg, Tore Julsrud; Appel, Silke; Lyssenko, Valeriya (Journal article; Peer reviewed, 2020)Type 1 diabetes (T1D) is largely considered an autoimmune disease leading to the destruction of insulin-producing pancreatic β cells. Further, patients with T1D have 3–4-fold increased risk of developing micro- and ... -
Inflammatory Stratification in Primary Sjögren’s Syndrome Reveals Novel Immune Cell Alterations in Patients’ Minor Salivary Glands
Bharaj, Tamandeep Kaur; Aqrawi, Lara A.; Fromreide, Siren; Jonsson, Roland; Brun, Johan G.; Appel, Silke; Skarstein, Kathrine (Journal article; Peer reviewed, 2021)There is a critical need to deconvolute the heterogeneity displayed by the minor salivary glands of primary Sjögren’s syndrome (pSS) patients. This is challenging primarily because the disease etiology remains unknown. The ... -
Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses
Hovden, Arnt-Ove; Karlsen, Marie; Jonsson, Roland; Aarstad, Hans Jørgen; Appel, Silke (Peer reviewed; Journal article, 2011-01-05)Background: Design of tumour specific immunotherapies using the patients’ own dendritic cells (DC) is a fast advancing scientific field. The functional qualities of the DC generated in vitro are critical, and today’s gold ... -
An optimized multiplex flow cytometry protocol for the analysis of intracellular signaling in peripheral blood mononuclear cells
Davies, Richard Allan; Vogelsang, Petra; Jonsson, Roland; Appel, Silke (Peer reviewed; Journal article, 2016-09)Phosphoflow cytometry is increasingly being used as a tool for the discovery of biomarkers used in the treatment and monitoring of disease and therapy. The ability to measure numerous phospho-protein targets simultaneously ... -
Single cell based phosphorylation profiling identifies alterations in toll-like receptor 7 and 9 signaling in patients with primary Sjögren's syndrome
Davies, Richard Allan; Sarkar, Irene; Hammenfors, Daniel; Bergum, Brith; Vogelsang, Petra; Solberg, Silje Michelsen; Gavasso, Sonia; Brun, Johan G; Jonsson, Roland; Appel, Silke (Peer reviewed; Journal article, 2019-02-21)Primary Sjögren's syndrome (pSS) is associated with polymorphisms and mRNA expression profiles that are indicative of an exaggerated innate and type I IFN immune response. Excessive activation potential of signaling pathways ... -
Variability of Primary Sjögren’s Syndrome Is Driven by Interferon-α and Interferon-α Blood Levels Are Associated With the Class II HLA–DQ Locus
Trutschel, Diana; Bost, Pierre; Mariette, Xavier; Bondet, Vincent; Llibre, Alba; Posseme, Celine; Charbit, Bruno; Thorball, Christian W.; Jonsson, Roland; Lessard, Christopher J.; Felten, Renaud; Ng, Wan Fai; Chatenoud, Lucienne; Dumortier, Hélène; Sibilia, Jean; Fellay, Jacques; Brokstad, Karl Albert; Appel, Silke; Tarn, Jessica R; Quintana-Murci, Lluis; Mingueneau, Michael; Meyer, Nicolas; Duffy, Darragh; Schwikowski, Benno; Gottenberg, Jacques Eric (Journal article; Peer reviewed, 2022)Objective Primary Sjögren's syndrome (SS) is the second most frequent systemic autoimmune disease, affecting 0.1% of the general population. To characterize the molecular and clinical variabilities among patients with ...