Browsing Bergen Open Research Archive by Author "Chera, Simona"
Now showing items 1-17 of 17
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Analyzing the impact of recurrent Kahoot tests on student performance
Chera, Simona (UPED-skrift, Working paper, 2020)Here we performed a small pilot study assessing the impact of recurrent kahoot quizzes on medical students’ performance in cutting-edge subjects (not in the typical curricula). Albeit several inherent design limitations, ... -
Bioinformatic analyses of miRNA-mRNA signature during hiPSC differentiation towards insulin-producing cells upon HNF4α mutation
Ghila, Luiza; Bjørlykke, Yngvild; Legøy, Thomas Aga; Vethe, Heidrun; Furuyama, Kenichiro; Chera, Simona; Ræder, Helge (Journal article; Peer reviewed, 2020)Mutations in the hepatocyte nuclear factor 4α (HNF4α) gene affect prenatal and postnatal pancreas development, being characterized by insulin-producing β-cell dysfunction. Little is known about the cellular and molecular ... -
Chronically elevated exogenous glucose elicits antipodal effects on the proteome signature of differentiating human iPSC-derived pancreatic progenitors
Ghila, Luiza; Legøy, Thomas Aga; Mathisen, Andreas Frøslev; Abadpour, Shadab; Paulo, Joao A.; Scholz, Hanne Bjørnson; Ræder, Helge; Chera, Simona (Journal article; Peer reviewed, 2021-04-02)The past decade revealed that cell identity changes, such as dedifferentiation or transdifferentiation, accompany the insulin-producing β-cell decay in most diabetes conditions. Mapping and controlling the mechanisms ... -
Circadian organization of lipid landscape is perturbed in type 2 diabetic patients
Sinturel, Flore; Chera, Simona; Brulhart-Meynet, Marie-Claude; Montoya, Jonathan Paz; Stenvers, Dirk Jan; Bisschop, Peter H.; Kalsbeek, Andries; Guessous, Idris; Jornayvaz, François R.; Philippe, Jacques; Brown, Steven A.; D'Angelo, Giovanni; Riezman, Howard; Dibner, Charna (Journal article; Peer reviewed, 2023)Lipid homeostasis in humans follows a diurnal pattern in muscle and pancreatic islets, altered upon metabolic dysregulation. We employ tandem and liquid-chromatography mass spectrometry to investigate daily regulation of ... -
The core clock transcription factor BMAL1 drives circadian β-cell proliferation during compensatory regeneration of the endocrine pancreas
Petrenko, Volodymyr; Stolovich-Rain, Miri; Vandereycken, Bart; Giovannoni, Laurianne; Storch, Kai-Florian; Dor, Yuval; Chera, Simona; Dibner, Charna (Journal article; Peer reviewed, 2020)Circadian clocks in pancreatic islets participate in the regulation of glucose homeostasis. Here we examined the role of these timekeepers in β-cell regeneration after the massive ablation of β cells by doxycycline-induced ... -
The effect of WnT pathway modulators on human iPSC-derived pancreatic beta cell maturation
Vethe, Heidrun; Ghila, Luiza; Berle, Magnus; Hoareau, Laurence; Haaland, Øystein Ariansen; Scholz, Hanne; Paulo, Joao A.; Chera, Simona; Ræder, Helge (Peer reviewed; Journal article, 2019-05-08)Current published protocols for targeted differentiation of human stem cells toward pancreatic β-cells fail to deliver sufficiently mature cells with functional properties comparable to human islet β-cells. We aimed to ... -
Encapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signalling
Legøy, Thomas Aga; Vethe, Heidrun; Abadpour, Shadab; Strand, Berit Løkensgard; Scholz, Hanne; Paulo, Joao A.; Ræder, Helge; Ghila, Luiza; Chera, Simona (Journal article; Peer reviewed, 2020)Cell replacement therapies hold great therapeutic potential. Nevertheless, our knowledge of the mechanisms governing the developmental processes is limited, impeding the quality of differentiation protocols. Generating ... -
In vivo environment swiftly restricts human pancreatic progenitors toward mono-hormonal identity via a HNF1A/HNF4A mechanism
Legøy, Thomas Aga; Mathisen, Andreas Frøslev; Salim, Zaidon; Vethe, Heidrun; Bjørlykke, Yngvild; Abadpour, Shadab; Paulo, Joao; Scholz, Hanne; Ræder, Helge; Ghila, Luiza; Chera, Simona (Journal article; Peer reviewed, 2020)Generating insulin-producing β-cells from human induced pluripotent stem cells is a promising cell replacement therapy for improving or curing insulin-dependent diabetes. The transplantation of end-stages differentiating ... -
In vivo hyperglycemia exposure elicits distinct period-dependent effects on human pancreatic progenitor differentiation, conveyed by oxidative stress
Legøy, Thomas Aga; Ghila, Luiza; Vethe, Heidrun; Abadpour, Shadab; Mathisen, Andreas; Paulo, Joao A.; Scholz, Hanne; Ræder, Helge; Chera, Simona (Peer reviewed; Journal article, 2020)Aim: The loss of insulin‐secreting β‐cells, ultimately characterizing most diabetes forms, demands the development of cell replacement therapies. The common endpoint for all ex vivo strategies is transplantation into ... -
Islet cell replacement and transplantation immunology in a mouse strain with inducible diabetes
Bhagchandani, Preksha; Chang, Charles A.; Zhao, Weichen; Ghila, Luiza Mihaela; Herrera, Pedro L.; Chera, Simona; Kim, Seung K. (Journal article; Peer reviewed, 2022)Improved models of experimental diabetes are needed to develop cell therapies for diabetes. Here, we introduce the B6 RIP-DTR mouse, a model of experimental diabetes in fully immunocompetent animals. These inbred mice ... -
Mapping Proteome Changes in Microsatellite Stable, Recurrent Colon Cancer Reveals a Significant Immune System Signature
Berle, Magnus; Hestetun, Kjersti Elvestad; Vethe, Heidrun; Chera, Simona; Paulo, Joao A.; Dahl, Olav; Myklebust, Mette Pernille (Journal article; Peer reviewed, 2022)Background/Aim: Better stratification of the risk of relapse will help select the right patients for adjuvant treatment and improve targeted therapies for patients with colon cancer. Materials and Methods: To understand ... -
Modulating Wnt signaling in human induced pluripotent stem cell-derived S7 cells
Vethe, Heidrun; Ghila, Luiza; Berle, Magnus; Hoareau, Laurence; Haaland, Øystein Ariansen; Scholz, Hanne; Paulo, Joao A.; Chera, Simona; Ræder, Helge (Peer reviewed; Journal article, 2019)Current published protocols for targeted differentiation of human stem cells toward pancreatic β-cells fail to deliver sufficiently mature cells with functional properties comparable to human islet β-cells. We aimed to ... -
Modulation of Unfolded Protein Response Restores Survival and Function of β-Cells Exposed to the Endocrine Disruptor Bisphenol A
Daian, Laura Maria; Tanko, Gabriela; Vacaru, Andrei Mircea; Ghila, Luiza Mihaela; Chera, Simona; Vacaru, Ana-Maria (Journal article; Peer reviewed, 2023-01-19)Diabetes is a metabolic disease that currently affects nearly half a billion people worldwide. β-cells dysfunction is one of the main causes of diabetes. Exposure to endocrine-disrupting chemicals is correlated with increased ... -
Molecular profiling of NOD mouse islets reveals a novel regulator of insulitis onset
Mathisen, Andreas Frøslev; Vacaru, Andrei Mircea; Unger, Lucas; Lamba, Elena Mirela; Mardare, Oana-Ana-Maria; Daian, Laura Maria; Ghila, Luiza Mihaela; Vacaru, Ana-Maria; Chera, Simona (Journal article; Peer reviewed, 2024)Non-obese diabetes (NOD) mice are an established, spontaneous model of type 1 diabetes in which diabetes develops through insulitis. Using next-generation sequencing, coupled with pathway analysis, the molecular fingerprint ... -
Reprogrammed Cells Display Distinct Proteomic SignaturesAssociated with Colony Morphology Variability
Bjørlykke, Yngvild; Søviknes, Anne Mette; Hoareau, Laurence; Vethe, Heidrun; Mathisen, Andreas; Chera, Simona; Vaudel, Marc; Ghila, Luiza; Ræder, Helge (Peer reviewed; Journal article, 2019-11-18)Human induced pluripotent stem cells (hiPSCs) are of high interest because they can be differentiated into a vast range of different cell types. Ideally, reprogrammed cells should sustain long-term culturing in an ... -
Stage-specific transcriptomic changes in pancreatic α-cells after massive β-cell loss
Oropeza, Daniel; Cigliola, Valentina; Romero, Agustín; Chera, Simona; Rodríguez-Seguí, Santiago; Herrera, Pedro L. (Journal article; Peer reviewed, 2021)Background Loss of pancreatic insulin-secreting β-cells due to metabolic or autoimmune damage leads to the development of diabetes. The discovery that α-cells can be efficiently reprogrammed into insulin-secreting cells ... -
Type 2 diabetes disrupts circadian orchestration of lipid metabolism and membrane fluidity in human pancreatic islets
Petrenko, Volodymyr; Sinturel, Flore; Loizides-Mangold, Ursula; Montoya, Jonathan Paz; Chera, Simona; Riezman, Howard; Dibner, Charna (Journal article; Peer reviewed, 2022-08-03)Recent evidence suggests that circadian clocks ensure temporal orchestration of lipid homeostasis and play a role in pathophysiology of metabolic diseases in humans, including type 2 diabetes (T2D). Nevertheless, circadian ...