• Comparing microbiota profiles in induced and spontaneous sputum samples in COPD patients 

      Tangedal, Solveig; Aanerud, Marianne; Grønseth, Rune; Drengenes, Christine; Wiker, Harald G; Bakke, Per S.; Eagan, Tomas Mikal (Peer reviewed; Journal article, 2017-08-29)
      Background: Induced and spontaneous sputum are used to evaluate the airways microbiota. Whether the sputum types can be used interchangeably in microbiota research is unknown. Our aim was to compare microbiota in induced ...
    • Exploring protocol bias in airway microbiome studies: one versus two PCR steps and 16S rRNA gene region V3 V4 versus V4 

      Drengenes, Christine; Eagan, Tomas Mikal; Haaland, Ingvild; Wiker, Harald G; Nielsen, Rune (Journal article; Peer reviewed, 2021)
      Background Studies on the airway microbiome have been performed using a wide range of laboratory protocols for high-throughput sequencing of the bacterial 16S ribosomal RNA (16S rRNA) gene. We sought to determine the ...
    • Laboratory contamination in airway microbiome studies 

      Drengenes, Christine; Wiker, Harald G; Kalananthan, Tharmini; Nordeide, Eli; Eagan, Tomas Mikal; Nielsen, Rune (Peer reviewed; Journal article, 2019-08-14)
      Background The low bacterial load in samples acquired from the lungs, have made studies on the airway microbiome vulnerable to contamination from bacterial DNA introduced during sampling and laboratory processing. We have ...
    • Methodological issues in airway microbiome studies 

      Drengenes, Christine (Doctoral thesis, 2020-12-04)
      Background Studies on the lung microbiome face unique methodological challenges tied to the low bacterial load of acquired samples and the increased susceptibility to bacterial DNA contamination. Contamination may be ...
    • Protected sampling is preferable in bronchoscopic studies of the airway microbiome 

      Grønseth, Rune; Drengenes, Christine; Wiker, Harald G; Tangedal, Solveig; Xue, Yaxin; Husebø, Gunnar; Svanes, Øistein; Lehmann, Sverre; Aardal, Marit Elisabeth; Hoang, Tuyen; Kalananthan, Tharmini; Martinsen, Einar Marius Hjellestad; Leiten, Elise Orvedal; Aanerud, Marianne; Nordeide, Eli; Haaland, Ingvild; Jonassen, Inge; Bakke, Per S.; Eagan, Tomas Mikal (Journal article; Peer reviewed, 2017)
      The aim was to evaluate susceptibility of oropharyngeal contamination with various bronchoscopic sampling techniques. 67 patients with obstructive lung disease and 58 control subjects underwent bronchoscopy with ...
    • The pulmonary mycobiome—A study of subjects with and without chronic obstructive pulmonary disease 

      Martinsen, Einar Marius Hjellestad; Eagan, Tomas Mikal Lind; Leiten, Elise Orvedal; Haaland, Ingvild; Husebø, Gunnar Reksten; Knudsen, Kristel Svalland; Drengenes, Christine; Sanseverino, Walter; Paytuví-Gallart, Andreu; Nielsen, Rune (Journal article; Peer reviewed, 2021-04-07)
      Background: The fungal part of the pulmonary microbiome (mycobiome) is understudied. We report the composition of the oral and pulmonary mycobiome in participants with COPD compared to controls in a large-scale single-centre ...