Browsing Bergen Open Research Archive by Author "Kursula, Inari"
Now showing items 1-12 of 12
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Analysis of Plasmodium falciparum myosin B ATPase activity and structure in complex with the calmodulin-like domain of its light chain MLC-B
Pires, Isa; Hung, Yu-Fu; Bergmann, Ulrich; Molloy, Justin E.; Kursula, Inari (Journal article; Peer reviewed, 2022)Myosin B (MyoB) is a class 14 myosin expressed in all invasive stages of the malaria parasite, Plasmodium falciparum. It is not associated with the glideosome complex that drives motility and invasion of host cells. During ... -
Apicomplexan actin polymerization depends on nucleation
Kumpula, Esa-Pekka; Pires, Isa; Lasiwa, Davki; Piirainen, Henni; Bergmann, Ulrich; Vahokoski, Juha; Kursula, Inari (Peer reviewed; Journal article, 2017-09-22)Filamentous actin is critical for apicomplexan motility and host cell invasion. Yet, parasite actin filaments are short and unstable. Their kinetic characterization has been hampered by the lack of robust quantitative ... -
Atomic view into plasmodium actin polymerization, ATP hydrolysis, and fragmentation
Kumpula, Esa-Pekka; Lopez Moreno, Andrea Johana; Tajedin, Leila; Han, Huijong; Kursula, Inari (Peer reviewed; Journal article, 2019)Plasmodium actins form very short filaments and have a noncanonical link between ATP hydrolysis and polymerization. Long filaments are detrimental to the parasites, but the structural factors constraining Plasmodium ... -
Biophysical analysis of Plasmodium falciparum Hsp70-Hsp90 organising protein (PfHop) reveals a monomer that is characterised by folded segments connected by flexible linkers
Makumire, Stanley; Zininga, Tawanda; Vahokoski, Juha; Kursula, Inari; Shonhai, Addmore (Journal article; Peer reviewed, 2020)Plasmodium falciparum causes the most lethal form of malaria. The cooperation of heat shock protein (Hsp) 70 and 90 is thought to facilitate folding of select group of cellular proteins that are crucial for cyto-protection ... -
Characterization of porphobilinogen deaminase mutants reveals that arginine-173 is crucial for polypyrrole elongation mechanism
Bustad, Helene J.; Kallio, Juha Pekka; Laitaoja, Mikko; Toska, Karen; Kursula, Inari; Martinez, Aurora; Jänis, Janne (Journal article; Peer reviewed, 2021)Porphobilinogen deaminase (PBGD), the third enzyme in the heme biosynthesis, catalyzes the sequential coupling of four porphobilinogen (PBG) molecules into a heme precursor. Mutations in PBGD are associated with acute ... -
Determinants of ligand binding and catalytic activity in the myelin enzyme 2′,3′-cyclic nucleotide 3′-phosphodiesterase
Raasakka, Arne; Myllykoski, Matti; Laulumaa, Saara; Lehtimäki, Mari; Härtlein, Michael; Moulin, Martine; Kursula, Inari; Kursula, Petri (Peer reviewed; Journal article, 2015-11-13)2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) is an enzyme highly abundant in the central nervous system myelin of terrestrial vertebrates. The catalytic domain of CNPase belongs to the 2H phosphoesterase superfamily ... -
A function of profilin in force generation during malaria parasite motility that is independent of actin binding
Moreau, Catherine A.; Quadt, Katharina A.; Piirainen, Henni; Kumar, Hirdesh; Bhargav, Saligram P.; Strauss, Léanne; Tolia, Niraj H; Wade, Rebecca C.; Spatz, Joachim P; Kursula, Inari; Frischknecht, Friedrich (Journal article; Peer reviewed, 2020)During transmission of malaria-causing parasites from mosquito to mammal, Plasmodium sporozoites migrate at high speed within the skin to access the bloodstream and infect the liver. This unusual gliding motility is based ... -
Genetic variation in metronidazole metabolism and oxidative stress pathways in clinical giardia lamblia assemblage A and B isolates
Saghaug, Christina Skår; Klotz, Christian; Kallio, Juha Pekka; Brattbakk, Hans-Richard; Stokowy, Tomasz; Aebischer, Toni; Kursula, Inari; Langeland, Nina; Hanevik, Kurt (Peer reviewed; Journal article, 2019-05-10)Purpose: Treatment-refractory Giardia cases have increased rapidly within the last decade. No markers of resistance nor a standardized susceptibility test have been established yet, but several enzymes and their pathways ... -
High-resolution structures of malaria parasite actomyosin and actin filaments
Vahokoski, Juha; Calder, Lesley J.; Moreno, Andrea Johana Lopez; Molloy, Justin E.; Kursula, Inari; Rosenthal, Peter B. (Journal article; Peer reviewed, 2022-04-04)Malaria is responsible for half a million deaths annually and poses a huge economic burden on the developing world. The mosquito-borne parasites (Plasmodium spp.) that cause the disease depend upon an unconventional ... -
The quaternary structure of human tyrosine hydroxylase: effects of dystonia‐associated missense variants on oligomeric state and enzyme activity
Szigetvari, Peter Daniel; Muruganandam, Gopinath; Kallio, Juha; Hallin, Erik Ingmar; Fossbakk, Agnete; Loris, Remy; Kursula, Inari; Møller, Lisbeth Birk; Knappskog, Per; Kursula, Petri; Haavik, Jan (Journal article; Peer reviewed, 2019)Tyrosine hydroxylase (TH) is a multi-domain, homo-oligomeric enzyme that catalyses the rate-limiting step of catecholamine neurotransmitter biosynthesis. Missense variants of human TH are associated with a recessive ... -
Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase
Muruganandam, Gopinath; Raasakka, Arne; Myllykoski, Matti; Kursula, Inari; Kursula, Petri (Peer reviewed; Journal article, 2017-05-16)Background: Eukaryotic tRNA splicing is an essential process in the transformation of a primary tRNA transcript into a mature functional tRNA molecule. 5′-phosphate ligation involves two steps: a healing reaction catalyzed ... -
Structure and function of an atypical homodimeric actin capping protein from the malaria parasite
Bendes, Ábris Ádám; Kursula, Petri; Kursula, Inari (Journal article; Peer reviewed, 2022)Apicomplexan parasites, such as Plasmodium spp., rely on an unusual actomyosin motor, termed glideosome, for motility and host cell invasion. The actin filaments are maintained by a small set of essential regulators, which ...