Browsing Bergen Open Research Archive by Author "Kursula, Petri"
Now showing items 1-20 of 41
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Antagonistic Functions of MBP and CNP Establish Cytosolic Channels in CNS Myelin
Snaidero, Nicolas; Velte, Caroline; Myllykoski, Matti; Raasakka, Arne; Ignatev, Alexander; Werner, Hauke B.; Erwig, Michelle S.; Möbius, Wiebke; Kursula, Petri; Nave, Klaus-Armin; Simons, Mikael (Peer reviewed; Journal article, 2017-01)The myelin sheath is a multilamellar plasma membrane extension of highly specialized glial cells laid down in regularly spaced segments along axons. Recent studies indicate that myelin is metabolically active and capable ... -
Cryo-EM, X-ray diffraction, and atomistic simulations reveal determinants for the formation of a supramolecular myelin-like proteolipid lattice
Ruskamo, Salla; Krokengen, Oda Caspara; Kowal, Julia; Nieminen, Tuomo; Lehtimäki, Mari; Raasakka, Arne; Dandey, Venkata P; Vattulainen, Ilpo; Stahlberg, Henning; Kursula, Petri (Journal article; Peer reviewed, 2020)Myelin protein P2 is a peripheral membrane protein of the fatty acid–binding protein family that functions in the formation and maintenance of the peripheral nerve myelin sheath. Several P2 gene mutations cause human ... -
Crystal and solution structure of NDRG1, a membranebinding protein linked to myelination and tumour suppression
Mustonen, Venla; Muruganandam, Gopinath; Loris, Remy; Kursula, Petri; Ruskamo, Salla (Journal article; Peer reviewed, 2021)N-myc downstream-regulated gene 1 (NDRG1) is a tumour suppressor involved in vesicular trafficking and stress response. NDRG1 participates in peripheral nerve myelination, and mutations in the NDRG1 gene lead to ... -
Crystal and solution structures reveal oligomerization of individual capsid homology domains of Drosophila Arc
Hallin, Erik Ingmar; Markusson, Sigurbjörn; Bottger, Lev; Torda, Andrew E.; Bramham, Clive Raymond Evjen; Kursula, Petri (Journal article; Peer reviewed, 2021)Synaptic plasticity is vital for brain function and memory formation. One of the key proteins in long-term synaptic plasticity and memory is the activity-regulated cytoskeleton-associated protein (Arc). Mammalian Arc forms ... -
Crystallographic home-source X-ray data for the atomic-resolution experimental phasing of the Shank3 SH3 domain structure from pseudomerohedrally twinned crystals
Ponna, Srinivas Kumar; Myllykoski, Matti; Kursula, Petri (Peer reviewed; Journal article, 2018-10)By far most macromolecular crystallographic data collection and experimental phasing is nowadays carried out using synchrotron radiation. Here, we present two crystallographic datasets collected on a home-source X-ray ... -
Determinants of ligand binding and catalytic activity in the myelin enzyme 2′,3′-cyclic nucleotide 3′-phosphodiesterase
Raasakka, Arne; Myllykoski, Matti; Laulumaa, Saara; Lehtimäki, Mari; Härtlein, Michael; Moulin, Martine; Kursula, Inari; Kursula, Petri (Peer reviewed; Journal article, 2015-11-13)2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) is an enzyme highly abundant in the central nervous system myelin of terrestrial vertebrates. The catalytic domain of CNPase belongs to the 2H phosphoesterase superfamily ... -
Development and Validation of Arc Nanobodies: New Tools for Probing Arc Dynamics and Function
Ishizuka, Yuta; Mergiya, Tadiwos Feyissa; Baldinotti, Rodolfo Da Silva Mazzarini; Xu, Ju; Hallin, Erik Ingmar; Markusson, Sigurbjörn; Kursula, Petri; Bramham, Clive Raymond Evjen (Journal article; Peer reviewed, 2022)Activity-regulated cytoskeleton-associated (Arc) protein plays key roles in long-term synaptic plasticity, memory, and cognitive flexibility. However, an integral understanding of Arc mechanisms is lacking. Arc is proposed ... -
Direct Binding of the Flexible C-Terminal Segment of Periaxin to β4 Integrin Suggests a Molecular Basis for CMT4F
Raasakka, Arne; Linxweiler, Helen; Brophy, Peter J; Sherman, Diane L; Kursula, Petri (Peer reviewed; Journal article, 2019-04-09)The process of myelination in the nervous system requires a coordinated formation of both transient and stable supramolecular complexes. Myelin-specific proteins play key roles in these assemblies, which may link membranes ... -
Exome sequencing in a child with neurodevelopmental disorder and epilepsy: Variant analysis of the AHNAK2 gene
Vinci, Mirella; Kursula, Petri; Greco, Donatella; Elia, Maurizio; Vetri, Luigi; Schepis, Carmelo; Chiavetta, Valeria; Donadio, Serena; Roccella, Michele; Carotenuto, Marco; Romano, Valentino; Calì, Francesco (Journal article; Peer reviewed, 2022)Background The AHNAK2 gene encodes a large nucleoprotein expressed in several tissues, including brain, squamous epithelia, smooth muscle, and neuropil. Its role in calcium signaling has been suggested and to date, clear ... -
Flexible Players within the Sheaths: The Intrinsically Disordered Proteins of Myelin in Health and Disease
Raasakka, Arne; Kursula, Petri (Journal article; Peer reviewed, 2020)Myelin ensheathes selected axonal segments within the nervous system, resulting primarily in nerve impulse acceleration, as well as mechanical and trophic support for neurons. In the central and peripheral nervous systems, ... -
GADL1 is a multifunctional decarboxylase with tissue-specific roles in β-alanine and carnosine production
Mahootchi, Elaheh; Homaei, Selina Cannon; Kleppe, Rune; Winge, Ingeborg; Hegvik, Tor-Arne; Perez, Roberto Megias; Totland, Christian; Mogavero, Floriana; Baumann, Anne; Glennon, Jeffrey Colm; Miletic, Hrvoje; Kursula, Petri; Haavik, Jan (Journal article; Peer reviewed, 2020)Carnosine and related β-alanine–containing peptides are believed to be important antioxidants, pH buffers, and neuromodulators. However, their biosynthetic routes and therapeutic potential are still being debated. This ... -
High-affinity anti-Arc nanobodies provide tools for structural and functional studies
Markusson, Sigurbjörn; Hallin, Erik Ingmar; Bustad, Helene J.; Raasakka, Arne; Xu, Ju; Muruganandam, Gopinath; Loris, Remy; Martinez, Aurora; Bramham, Clive Raymond Evjen; Kursula, Petri (Journal article; Peer reviewed, 2022)Activity-regulated cytoskeleton-associated protein (Arc) is a multidomain protein of retroviral origin with a vital role in the regulation of synaptic plasticity and memory formation in mammals. However, the mechanistic ... -
How Does Protein Zero Assemble Compact Myelin?
Raasakka, Arne; Kursula, Petri (Journal article; Peer reviewed, 2020-08)Myelin protein zero (P0), a type I transmembrane protein, is the most abundant protein in peripheral nervous system (PNS) myelin—the lipid-rich, periodic structure of membrane pairs that concentrically encloses long axonal ... -
Human myelin protein P2: from crystallography to time-lapse membrane imaging and neuropathy-associated variants
Uusitalo, Maiju; Klenow, Martin Berg; Laulumaa, Saara; Blakeley, Matthew P.; Simonsen, Adam Cohen; Ruskamo, Salla; Kursula, Petri (Journal article; Peer reviewed, 2021)Peripheral myelin protein 2 (P2) is a fatty acid-binding protein expressed in vertebrate peripheral nervous system myelin, as well as in human astrocytes. Suggested functions of P2 include membrane stacking and lipid ... -
Human myelin proteolipid protein structure and lipid bilayer stacking
Ruskamo, Salla; Raasakka, Arne; Pedersen, Jan Skov; Martel, Anne; Škubník, Karel; Darwish, Tamim; Porcar, Lionel; Kursula, Petri (Journal article; Peer reviewed, 2022)The myelin sheath is an essential, multilayered membrane structure that insulates axons, enabling the rapid transmission of nerve impulses. The tetraspan myelin proteolipid protein (PLP) is the most abundant protein of ... -
Ionic strength and calcium regulate membrane interactions of myelin basic protein and the cytoplasmic domain of myelin protein zero
Raasakka, Arne; Jones, Nykola C; Hoffmann, Søren V; Kursula, Petri (Peer reviewed; Journal article, 2019)The formation of a mature myelin sheath in the vertebrate nervous system requires specific protein-membrane interactions. Several myelin-specific proteins are involved in stacking lipid membranes into multilayered structures ... -
Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line
Raasakka, Arne; Ruskamo, Salla; Kowal, Julia; Barker, Robert; Baumann, Anne; Martel, Anne; Tuusa, Jussi; Myllykoski, Matti; Bürck, Jochen; Ulrich, Anne S.; Stahlberg, Henning; Kursula, Petri (Peer reviewed; Journal article, 2017-07-10)Compact myelin comprises most of the dry weight of myelin, and its insulative nature is the basis for saltatory conduction of nerve impulses. The major dense line (MDL) is a 3-nm compartment between two cytoplasmic leaflets ... -
Molecular mechanisms of Charcot-Marie-Tooth neuropathy linked to mutations in human myelin protein P2
Ruskamo, Salla; Nieminen, Tuomo; Kristiansen, Cecilie Katrin; Vatne, Guro Helén; Baumann, Anne; Hallin, Erik Ingmar; Raasakka, Arne; Joensuu, Päivi; Bergmann, Ulrich; Vattulainen, Ilpo; Kursula, Petri (Peer reviewed; Journal article, 2017-07-26)Charcot-Marie-Tooth (CMT) disease is one of the most common inherited neuropathies. Recently, three CMT1-associated point mutations (I43N, T51P, and I52T) were discovered in the abundant peripheral myelin protein P2. These ... -
Molecular structure and function of myelin protein P0 in membrane stacking
Raasakka, Arne; Ruskamo, Salla; Kowal, Julia; Han, Huijong; Baumann, Anne; Myllykoski, Matti; Fasano, Anna; Rossano, Rocco; Riccio, Paolo; Bürck, Jochen; Ulrich, Anne S.; Stahlberg, Henning; Kursula, Petri (Peer reviewed; Journal article, 2019)Compact myelin forms the basis of nerve insulation essential for higher vertebrates. Dozens of myelin membrane bilayers undergo tight stacking, and in the peripheral nervous system, this is partially enabled by myelin ... -
Multiple sclerosis and myelin basic protein: insights into protein disorder and disease
Martinsen, Vebjørn; Kursula, Petri (Journal article; Peer reviewed, 2022)Myelin basic protein (MBP) is an abundant protein in central nervous system (CNS) myelin. MBP has long been studied as a factor in the pathogenesis of the autoimmune neurodegenerative disease multiple sclerosis (MS). MS ...