• An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth 

      Egge, Elianne Dunthorn; Elferink, Stephanie; Vaulot, Daniel; John, Uwe; Bratbak, Gunnar; Larsen, Aud; Edvardsen, Bente (Journal article, 2021)
      Arctic marine protist communities have been understudied due to challenging sampling conditions, in particular during winter and in deep waters. The aim of this study was to improve our knowledge on Arctic protist diversity ...
    • Asynchronous Accumulation of Organic Carbon and Nitrogen in the Atlantic Gateway to the Arctic Ocean 

      Paulsen, Maria Lund; Seuthe, Lena; Reigstad, Marit; Larsen, Aud; Cape, Matthias; Vernet, Maria (Peer reviewed; Journal article, 2018-11-15)
      Nitrogen (N) is the main limiting nutrient for biological production in the Arctic Ocean. While dissolved inorganic N (DIN) is well studied, the substantial pool of N bound in organic matter (OM) and its bioavailability ...
    • Bacterial community composition responds to changes in copepod abundance and alters ecosystem function in an Arctic mesocosm study 

      Tsagaraki, Tatiana Margo; Pree, Bernadette; Leiknes, Øystein; Larsen, Aud; Bratbak, Gunnar; Øvreås, Lise; Egge, Jorun Karin; Spanek, Roman; Paulsen, Maria Lund; Olsen, Yngvar; Vadstein, Olav; Thingstad, T. Frede (Peer reviewed; Journal article, 2018)
      Combining a minimum food web model with Arctic microbial community dynamics, we have suggested that top-down control by copepods can affect the food web down to bacterial consumption of organic carbon. Pursuing this ...
    • Biological transformation of Arctic dissolved organic matter in a NE Greenland fjord 

      Paulsen, Maria Lund; Müller, Oliver; Larsen, Aud; Møller, Eva Friis; Middelboe, Mathias; Sejr, Mikael Kristian; Stedmon, Colin A. (Peer reviewed; Journal article, 2018-12-07)
      Arctic waters are often enriched with terrestrial dissolved organic matter (DOM) characterized by having elevated visible wavelength fluorescence (commonly termed humic-like). Here, we have identified the sources of ...
    • Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms 

      Lindivat, Mathilde; Larsen, Aud; Hess-Erga, Ole-Kristian; Bratbak, Gunnar; Hoell, Ingunn (Journal article; Peer reviewed, 2020)
      In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes. Enumeration of active ...
    • Carbon Bioavailability in a High Arctic Fjord Influenced by Glacial Meltwater, NE Greenland 

      Paulsen, Maria Lund; Nielsen, Sophia Elisabeth Bardram; Müller, Oliver; Møller, Eva Friis; Stedmon, Colin A.; Juul-Pedersen, Thomas; Markager, Stiig; Sejr, Mikael Kristian; Delgado Huertas, Antonio; Larsen, Aud; Middelboe, Mathias (Peer reviewed; Journal article, 2017-06-08)
      The land-to-ocean flux of organic carbon is increasing in glacierized regions in response to increasing temperatures in the Arctic (Hood et al., 2015). In order to understand the response of the coastal ecosystem metabolism ...
    • Contrasting response to nutrient manipulation in Arctic mesocosms are reproduced by a minimum microbial food web model 

      Larsen, Aud; Egge, Jorun Karin; Nejstgaard, Jens Christian; Di Capua, Iole; Thyrhaug, Runar; Bratbak, Gunnar; Thingstad, Tron Frede (Peer reviewed; Journal article, 2015-01-27)
      A minimum mathematical model of the marine pelagic microbial food web has previously shown to be able to reproduce central aspects of observed system response to different bottom-up manipulations in a mesocosm experiment ...
    • Differential toxicity of bioorthogonal non-canonical amino acids (BONCAT) in Escherichia coli 

      Landor, Lotta Anni Ingeborg; Bratbak, Gunnar; Larsen, Aud; Tjendra, Jesslyn; Våge, Selina (Journal article; Peer reviewed, 2023)
      Single-cell methods allow studying the activity of single bacterial cells, potentially shedding light on regulatory mechanisms involved in services like biochemical cycling. Bioorthogonal non-canonical amino acid tagging ...
    • Dual staining with CFDA-AM and SYTOX Blue in flow cytometry analysis of UV-irradiated Tetraselmis suecica to evaluate vitality 

      Olsen, Ranveig Ottøy; Hess-Erga, Ole-Kristian; Larsen, Aud; Hoffmann, Friederike; Thuestad, Gunnar; Hoell, Ingunn Alne (Peer reviewed; Journal article, 2016)
      After disinfection of ballast water, it is crucial to detect organisms and determine their vitality to assess the performance of the chosen treatment technique. Ultraviolet (UV) irradiation is a treatment technology commonly ...
    • Emerging interaction patterns in the Emiliania huxleyi-EhV system 

      Ruiz Martinez, Eliana; Oosterhof, Monique; Sandaa, Ruth-Anne; Larsen, Aud; Pagarete, António (Journal article, 2017-03-22)
      Viruses are thought to be fundamental in driving microbial diversity in the oceanic planktonic realm. That role and associated emerging infection patterns remain particularly elusive for eukaryotic phytoplankton and their ...
    • Flow Cytometric Analysis of Bacterial Protein Synthesis: Monitoring Vitality After Water Treatment 

      Lindivat, Mathilde; Bratbak, Gunnar; Larsen, Aud; Hess-Erga, Ole-Kristian; Hoell, Ingunn Alne (Journal article; Peer reviewed, 2021)
      Bacterial vitality after water disinfection treatment was investigated using bio-orthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM). Protein synthesis activity and DNA integrity (BONCAT–SYBR ...
    • High CO2 concentration and iron availability determine the metabolic inventory in an Emiliania huxleyi-dominated phytoplankton community 

      Mausz, Michaela A.; Segovia, María; Larsen, Aud; Berger, Stella Angela; Egge, Jorun Karin; Pohnert, Georg (Journal article; Peer reviewed, 2020)
      Ocean acidification (OA), a consequence of anthropogenic carbon dioxide (CO2) emissions, strongly impacts marine ecosystems. OA also influences iron (Fe) solubility, affecting biogeochemical and ecological processes. We ...
    • How microbial food web interactions shape the arctic ocean bacterial community revealed by size fractionation experiments 

      Müller, Oliver; Seuthe, Lena; Pree, Bernadette; Bratbak, Gunnar; Larsen, Aud; Paulsen, Maria Lund (Journal article; Peer reviewed, 2021-11-17)
      In the Arctic, seasonal changes are substantial, and as a result, the marine bacterial community composition and functions differ greatly between the dark winter and light-intensive summer. While light availability is, ...
    • Molecular gut content analysis demonstrates that Calanus grazing on Phaeocystis pouchetii and Skeletonema marinoi is sensitive to bloom phase but not prey density 

      Ray, Jessica Louise; Skaar, Katrine Sandnes; Simonelli, Paolo; Larsen, Aud; Troedsson, Christofer (Peer reviewed; Journal article, 2016-01-19)
      Mesozooplankton grazing selection in complex marine microbial communities is a poorly understood yet critical structuring component of marine microbial food webs. We wished to quantitatively assess how relative grazing by ...
    • Multiomics in the central Arctic Ocean for benchmarking biodiversity change 

      Mock, Thomas; Boulton, William; Balmonte, John-Paul; Barry, Kevin; Bertilsson, Stefan; Bowman, Jeff; Buck, Moritz; Bratbak, Gunnar; Chamberlain, Emelia J.; Cunliffe, Michael; Creamean, Jessie; Ebenhöh, Oliver; Eggers, Sarah Lena; Fong, Allison A.; Gardner, Jessie; Gradinger, Rolf Rudolf; Granskog, Mats A.; Havermans, Charlotte; Hill, Thomas; Hoppe, Clara J.M.; Korte, Kerstin; Larsen, Aud; Müller, Oliver; Nicolaus, Anja; Oldenburg, Ellen; Popa, Ovidiu; Rogge, Swantje; Schäfer, Hendrik; Shoemaker, Katyanne; Snoeijs-Leijonmalm, Pauline; Torstensson, Anders; Valentin, Klaus; Vader, Anna; Barry, Kerrie; Chen, I-M A; Clum, Alicia; Copeland, Alex; Daum, Chris; Eloe-Fadrosh, Emiley; Foster, Brian; Foster, Bryce; Grigoriev, Igor V.; Huntemann, Marcel; Ivanova, Natalia; Kuo, Alan; Kyrpides, Nikos C.; Mukherjee, Supratim; Palaniappan, Krishnaveni; Reddy, T.B.K.; Salamov, Asaf; Roux, Simon; Varghese, Neha; Woyke, Tanja; Wu, Dongying; Leggett, Richard M.; Moulton, Vincent; Metfies, Katja (Journal article; Peer reviewed, 2022-10-17)
      Multiomics approaches need to be applied in the central Arctic Ocean to benchmark biodiversity change and to identify novel species and their genes. As part of MOSAiC, EcoOmics will therefore be essential for conservation ...
    • Overview of the MOSAiC expedition: Ecosystem 

      Fong, Allison A.; Hoppe, Clara J. M.; Aberle, Nicole; Ashjian, Carin; Assmy, Philipp Kurt Wolf; Bai, Youcheng; Bakker, Dorothee C. E.; Balmonte, John Paul; Barry, Kevin; Bertilsson, Stefan; Boulton, William; Bowman, Jeff; Bozzato, Deborah; Bratbak, Gunnar; Buck, Moritz; Campbell, Robert G.; Castellani, Giulia; Chamberlain, Emelia J.; Chen, Jianfang; Chierici, Melissa; Cornils, Astrid; Creamean, Jessie; Damm, Ellen; Dethloff, Klaus; Droste, Elise S.; Ebenhöh, Oliver; Eggers, Sarah Lena; Engel, Anja; Flores, Hauke; Fransson, Agneta; Frickenhaus, Stephan; Gardner, Jessie; Gelfman, Cecilia E.; Granskog, Mats; Graeve, Martin; Havermans, Charlotte; Heuzé, Céline; Hildebrandt, Nicole; Hill, Thomas C. J.; Hoppema, Mario; Immerz, Antonia; Jin, Haiyan; Koch, Boris P.; Kong, Xianyu; Kraberg, Alexandra C.; Lan, Musheng; Lange, Benjamin Allen; Larsen, Aud; Lebreton, Benoit; Leu, Eva Susanne; Loose, Brice; Maslowski, Wieslaw; Mavis, Camille; Metfies, Katja; Mock, Thomas; Müller, Oliver; Marcel, Nicolaus; Niehoff, Barbara; Nomura, Daiki; Nöthig, Eva-Maria; Oggier, Marc; Oldenburg, Ellen; Olsen, Lasse Mork; Peeken, Ilka; Perovich, Donald K.; Popa, Ovidiu; Rabe, Benjamin; Ren, Jian; Rex, Markus; Rinke, Annette; Rokitta, Sebastian; Rost, Björn; Sakinan, Serdar S.; Salganik, Evgenii; Schaafsma, Fokje L.; Schäfer, Hendrik; Schmidt, Katrin; Shoemaker, Katyanne; Shupe, Matthew D.; Snoeijs-Leijonmalm, Pauline; Stefels, Jacqueline; Svenson, Anders; Tao, Ran; Sinhué, Torres-Valdés; Torstensson, Anders; Toseland, Andrew; Ulfsbo, Adam; Leeuwe, Maria A. van; Vortkamp, Martina; Webb, Alison L.; Zhuang, Yanpei; Gradinger, Rolf Rudolf (Journal article; Peer reviewed, 2024)
      The international and interdisciplinary sea-ice drift expedition “The Multidisciplinary drifting Observatory for the Study of Arctic Climate” (MOSAiC) was conducted from October 2019 to September 2020. The aim of MOSAiC ...
    • Phaeoviruses Present in Cultured and Natural Kelp Species, Saccharina latissima and Laminaria hyperborea (Phaeophyceae, Laminariales), in Norway 

      Ruiz Martinez, Eliana; Mckeown, Dean A.; Schroeder, Decland C.; Thuestad, Gunnar; Sjøtun, Inga Kjersti; Sandaa, Ruth-Anne; Larsen, Aud; Hoell, Ingunn Alne (Journal article; Peer reviewed, 2023)
      Phaeoviruses (Phycodnaviridae) are large icosahedral viruses in the phylum Nucleocytoviricota with dsDNA genomes ranging from 160 to 560 kb, infecting multicellular brown algae (Phaeophyceae). The phaeoviral host range is ...
    • Photosynthetic light requirement near the theoretical minimum detected in Arctic microalgae 

      Hoppe, Clara J. M.; Fuchs, Niels; Notz, Dirk; Anderson, Philip; Assmy, Philipp Kurt Wolf; Berge, Jørgen; Bratbak, Gunnar; Guillou, Gaël; Kraberg, Alexandra; Larsen, Aud; Lebreton, Benoit; Leu, Eva; Lucassen, Magnus; Müller, Oliver; Oziel, Laurent; Rost, Björn; Schartmüller, Bernhard; Torstensson, Anders; Wloka, Jonas (Journal article; Peer reviewed, 2024)
      Photosynthesis is one of the most important biological processes on Earth, providing the main source of bioavailable energy, carbon, and oxygen via the use of sunlight. Despite this importance, the minimum light level ...
    • The potential of sedimentary ancient DNA for reconstructingpast sea ice evolution 

      De Schepper, Stijn; Ray, Jessica Louise; Skaar, Katrine Sandnes; Sadatzki, Henrik; Ijaz, Umer Zeeshan; Stein, Ruediger; Larsen, Aud (Peer reviewed; Journal article, 2019-06-24)
      Sea ice is a crucial component of the Arctic climate system, yet the tools to document the evolution of sea ice conditions onhistorical and geological time scales are few and have limitations. Such records are essential ...
    • Removal of large viruses and their dispersal through fecal pellets of the appendicularian Oikopleura dioica during Emiliania huxleyi bloom conditions 

      Mayers, Kyle; Lawrence, Janice; Skaar, Katrine Sandnes; Töpper, Joachim Paul; Petelenz-Kurdziel, Elzbieta Anna; Saltvedt, Marius Rydningen; Sandaa, Ruth-Anne; Larsen, Aud; Bratbak, Gunnar; Ray, Jessica Louise (Journal article; Peer reviewed, 2021)
      Despite their importance in shaping the structure and function of marine microbial food webs, little is known about factors regulating marine virus abundance. Previous work demonstrated clearance of laboratory-cultured ...