Browsing Bergen Open Research Archive by Author "Lie, Kai Kristoffer"
Now showing items 1-15 of 15
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Analysis of immunoglobulin and T cell receptor gene expression in ballan wrasse (Labrus bergylta) revealed an extraordinarily high IgM expression in the gut
Bilal, Sumaira; Lie, Kai Kristoffer; Dalum, Alf Seljenes; Karlsen, Odd Andre; Hordvik, Ivar (Peer reviewed; Journal article, 2019)The serum IgM concentration of ballan wrasse is relatively high, estimated to approximately 13 mg/ml in adult wild fish of 800 g. The present study revealed an unusual high abundance of IgM mRNA in the gut of ballan wrasse. ... -
Co-exposure to UV radiation and crude oil increases acute embryotoxicity and sublethal malformations in the early life stages of Atlantic haddock (Melanogrammus aeglefinus)
Sørhus, Elin; Donald, Carey; Nakken, Charlotte L.; Perrichon, Prescilla; Durif, Caroline; Shema, Steven; Browman, Howard; Skiftesvik, Anne Berit; Lie, Kai Kristoffer; Rasinger, Josef; Müller, Mette Helen Bjørge; Meier, Sonnich (Journal article; Peer reviewed, 2023)Crude oil causes severe abnormalities in developing fish. Photomodification of constituents in crude oil increases its toxicity several fold. We report on the effect of crude oil, in combination with ultraviolet (UV) ... -
Dietary selenomethionine reduce mercury tissue levels and modulate methylmercury induced proteomic and transcriptomic alterations in hippocampi of adolescent BALB/c mice
Mellingen, Ragnhild Marie; Myrmel, Lene Secher; Rasinger, Josef; Lie, Kai Kristoffer; Bernhard, Annette; Madsen, Lise; Nøstbakken, Ole Jakob (Journal article; Peer reviewed, 2022-10-13)Methylmercury (MeHg) is a well-known environmental contaminant, particularly harmful to the developing brain. The main human dietary exposure to MeHg occurs through seafood consumption. However, seafood also contains several ... -
Effects of cholecystokinin (CCK) on gut motility in the stomachless fish ballan wrasse (Labrus bergylta)
Le, Hoang Thi My Dung; Lie, Kai Kristoffer; Giroud-Argoud, Justine; Rønnestad, Ivar; Sæle, Øystein (Peer reviewed; Journal article, 2019-06-07)Cholecystokinin (CCK) is well-known as a key hormone that inhibits stomach emptying and stimulates midgut motility in gastric species. However, the function of CCK related to gut motility in agastric fish, especially in ... -
Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon
Olsvik, Pål A.; Lie, Kai Kristoffer; Olderbakk Jordal, Anne-Lise; Nilsen, Tom Ole; Hordvik, Ivar (Peer reviewed; Journal article, 2005)Background: Salmonid fishes are among the most widely studied model fish species but reports on systematic evaluation of reference genes in qRT-PCR studies is lacking. Results: The stability of six potential reference genes ... -
Intestinal function of the stomachless fish, ballan wrasse (Labrus bergylta)
Le, Hoang Thi My Dung; Shao, Xinting; Krogdahl, Åshild; Kortner, Trond Moxness; Lein, Ingrid; Kousoulaki, Katerina; Lie, Kai Kristoffer; Sæle, Øystein (Peer reviewed; Journal article, 2019-03-26)Little is known about the digestion of nutrients in stomachless (agastric) fishes with short intestines, such as wrasse. This study describes the digestion, absorption and evacuation rates in ballan wrasse (Labrus bergylta) ... -
Loss of stomach, loss of appetite? Sequencing of the ballan wrasse (Labrus bergylta) genome and intestinal transcriptomic profiling illuminate the evolution of loss of stomach function in fish
Lie, Kai Kristoffer; Tørresen, Ole K.; Solbakken, Monica Hongrø; Rønnestad, Ivar; Tooming-Klunderud, Ave; Nederbragt, Alexander Johan; Jentoft, Sissel; Sæle, Øystein (Peer reviewed; Journal article, 2018-03-06)Background: The ballan wrasse (Labrus bergylta) belongs to a large teleost family containing more than 600 species showing several unique evolutionary traits such as lack of stomach and hermaphroditism. Agastric fish are ... -
The ontogeny of lymphoid organs and IgM+ B-cells in ballan wrasse (Labrus bergylta) reveals a potential site for extrarenal B-cell lymphopoiesis: The pancreas
Etayo, Angela; Bjørgen, Håvard; Koppang, Erling Olaf; Lie, Kai Kristoffer; Bjelland, Reidun Marie; Hordvik, Ivar; Øvergård, Aina-Cathrine; Sæle, Øystein (Journal article; Peer reviewed, 2024)Vaccination of farmed fish is the most effective prophylactic measure against contagious diseases but requires specific knowledge on when the adaptive immune system is fully developed. The present work describes kidney and ... -
Ossification of Atlantic cod (Gadus morhua) – Developmental stages revisited
Sæle, Øystein; Haugen, Trine; Karlsen, Ørjan; van der Meeren, Terje; Bæverfjord, Grete; Hamre, Kristin; Rønnestad, Ivar; Moren, Mari; Lie, Kai Kristoffer (Peer reviewed; Journal article, 2017-02)In studies of marine larvae, it is common to use days post-hatch as a developmental reference point. We show that age is a poor measure of morphological and physiological development in Atlantic cod. Therefore, we propose ... -
Pharmacokinetics and transcriptional effects of the anti-salmon lice drug emamectin benzoate in Atlantic salmon (Salmo salar L.)
Olsvik, Pål Asgeir; Lie, Kai Kristoffer; Mykkeltvedt, Eva; Samuelsen, Ole Bent; Petersen, Kjell; Stavrum, Anne-Kristin; Lunestad, Bjørn Tore (Peer reviewed; Journal article, 2008-09-11)Background: Emamectin benzoate (EB) is a dominating pharmaceutical drug used for the treatment and control of infections by sea lice (Lepeophtheirus salmonis) on Atlantic salmon (Salmo salar L). Fish with an initial mean ... -
Photo-enhanced toxicity of crude oil on early developmental stages of Atlantic cod (Gadus morhua)
Aranguren Abadía, Libe; Yadetie, Fekadu; Donald, Carey; Sørhus, Elin; Myklatun, Lars Eirik; Zhang, Xiaokang; Lie, Kai Kristoffer; Perrichon, Prescilla; Nakken, Charlotte Langeland; Durif, Caroline; Shema, Steven; Browman, Howard; Skiftesvik, Anne Berit; Goksøyr, Anders; Meier, Sonnich; Karlsen, Odd André (Journal article; Peer reviewed, 2022)Photo-enhanced toxicity of crude oil is produced by exposure to ultraviolet (UV) radiation. Atlantic cod (Gadus morhua) embryos were exposed to crude oil with and without UV radiation (290–400 nm) from 3 days post fertilization ... -
Physical and nutrient stimuli differentially modulate gut motility patterns, gut transit rate, and transcriptome in an agastric fish, the ballan wrasse
Le, Hoang Thi My Dung; Lie, Kai Kristoffer; Etayo, Angela; Rønnestad, Ivar; Sæle, Øystein (Journal article; Peer reviewed, 2021-02-11)The effects of nutrient and mechanical sensing on gut motility and intestinal metabolism in lower vertebrates remains largely unknown. Here we present the transcriptome response to luminal stimulation by nutrients and an ... -
RNA sequencing and proteomic profiling reveal different alterations by dietary methylmercury in the hippocampal transcriptome and proteome in BALB/c mice
Mellingen, Ragnhild Marie; Myrmel, Lene Secher; Lie, Kai Kristoffer; Rasinger, Josef; Madsen, Lise; Nøstbakken, Ole Jakob (Journal article; Peer reviewed, 2021)Methylmercury (MeHg) is a highly neurotoxic form of mercury (Hg) present in seafood. Here, we recorded and compared proteomic and transcriptomic changes in hippocampus of male BALB/c mice exposed to two doses of MeHg. Mice ... -
T cell receptor alpha chain genes in the teleost Ballan wrasse (Labrus bergylta) are subjected to somatic hypermutation
Bilal, Sumaira; Lie, Kai Kristoffer; Sæle, Øystein; Hordvik, Ivar (Peer reviewed; Journal article, 2018-05-22)Previously, somatic hypermutation (SHM) was considered to be exclusively associated with affinity maturation of antibodies, although it also occurred in T cells under certain conditions. More recently, it has been shown ... -
The thymus and T-cell ontogeny in ballan wrasse (Labrus bergylta) is nutritionally modelled
Etayo, Angela; Lie, Kai Kristoffer; Bjelland, Reidun Marie; Hordvik, Ivar; Øvergård, Aina-Cathrine; Sæle, Øystein (Journal article; Peer reviewed, 2023-05-01)Marine fish larvae often experience high mortality unrelated to predation during early life stages, and farmed ballan wrasse (Labrus bergylta) is no exception. Knowing when the adaptive immune system is developed and fully ...