Browsing Bergen Open Research Archive by Author "Lorens, James B."
Now showing items 1-8 of 8
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Automated Quantification and Integrative Analysis of 2D and 3D Mitochondrial Shape and Network Properties
Nikolaisen, Julie; Nilsson, Linn Iren Hodneland; Pettersen, Ina Katrine Nitschke; Willems, Peter H. G. M.; Lorens, James B.; Koopman, Werner J. H.; Tronstad, Karl Johan (Peer reviewed; Journal article, 2014-07-02)Mitochondrial morphology and function are coupled in healthy cells, during pathological conditions and (adaptation to) endogenous and exogenous stress. In this sense mitochondrial shape can range from small globular ... -
Endothelial microvascular networks affect gene-expression profiles and osteogenic potential of tissue-engineered constructs
Pedersen, Torbjørn Østvik; Blois, Anna L; Xing, Zhe; Xue, Ying; Sun, Yang; Finne-Wistrand, Anna; Akslen, Lars A.; Lorens, James B.; Leknes, Knut N.; Fristad, Inge; Mustafa, Kamal Babikeir Eln (Peer reviewed; Journal article, 2013-05-17)Introduction: A major determinant of the potential size of cell/scaffold constructs in tissue engineering is vascularization. The aims of this study were twofold: first to determine the in vitro angiogenic and osteogenic ... -
Enhanced gene expression from retroviral vectors
Blø, Magnus; Micklem, David R.; Lorens, James B. (Peer reviewed; Journal article, 2008-02-25)Background: Retroviruses are widely used to transfer genes to mammalian cells efficiently and stably. However, genetic elements required for high-level gene expression are incompatible with standard systems. The retroviral ... -
Evaluating Extracellular Matrix influence on adherent cell signaling by Cold Trypsin Phosphorylation-specific Flow Cytometry
Abrahamsen, Iren; Lorens, James B. (Peer reviewed; Journal article, 2013-08-19)Background: Tissue microenvironments comprise different extracellular matrix (ECM) proteins that regulate cellular responsiveness to growth factors. In vitro culture of adherent cells on ECM-coated substrata is commonly ... -
Flow cytometry-based functional selection of RNA interference triggers for efficient epi-allelic analysis of therapeutic targets
Micklem, David Robert; Blø, Magnus; Bergström, Petra; Hodneland, Erlend; Tiron, Crina Elena; Høiby, Torill; Gjerdrum, Christine; Hammarsten, Ola; Lorens, James B. (Peer reviewed; Journal article, 2014-06-21)Background: The dose-response relationship is a fundamental pharmacological parameter necessary to determine therapeutic thresholds. Epi-allelic hypomorphic analysis using RNA interference (RNAi) can similarly correlate ... -
High-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia
Pelissier, Fanny; Schapiro, Denis; Chang, Hang; Borowsky, Alexander D.; Lee, Jonathan K.; Parvin, Bahram; Stampfer, Martha R.; LaBarge, Mark A.; Bodenmiller, Bernd; Lorens, James B. (Peer reviewed; Journal article, 2018-04)Aging is associated with tissue-level changes in cellular composition that are correlated with increased susceptibility to disease. Aging human mammary tissue shows skewed progenitor cell potency, resulting in diminished ... -
Mesenchymal stem cells induce endothelial cell quiescence and promote capillary formation
Pedersen, Torbjørn Østvik; Blois, Anna L; Xue, Ying; Xing, Zhe; Sun, Yang; Finne-Wistrand, Anna; Lorens, James B.; Fristad, Inge; Leknes, Knut N.; Mustafa, Kamal Babikeir Eln (Peer reviewed; Journal article, 2014-02-17)Introduction: Rapid establishment of functional blood vessels is a prerequisite for successful tissue engineering. During vascular development, endothelial cells (ECs) and perivascular cells assemble into a complex regulating ... -
Rapid and reliable cloning of PCR products
Lorens, James B. (Peer reviewed; Journal article, 1991-08-08)