Browsing Bergen Open Research Archive by Author "Lorens, James B."

Now showing items 1-9 of 9

    • Automated Quantification and Integrative Analysis of 2D and 3D Mitochondrial Shape and Network Properties 

      Nikolaisen, Julie; Nilsson, Linn Iren Hodneland; Pettersen, Ina Katrine Nitschke; Willems, Peter H. G. M.; Lorens, James B.; Koopman, Werner J. H.; Tronstad, Karl Johan (Peer reviewed; Journal article, 2014-07-02)
      Mitochondrial morphology and function are coupled in healthy cells, during pathological conditions and (adaptation to) endogenous and exogenous stress. In this sense mitochondrial shape can range from small globular ...

    • Axl-inhibitor bemcentinib alleviates mitochondrial dysfunction in the unilateral ureter obstruction murine model 

      Hoel, August; Osman, Tarig; Hoel, Fredrik; Elsaid, Hassan Osman Alhassan; Chen, Tony; Landolt, Lea Zoe; Babickova, Janka; Tronstad, Karl Johan; Lorens, James B.; Gausdal, Gro; Marti, Hans-Peter; Furriol, Jessica (Journal article; Peer reviewed, 2021)
      Renal fibrosis is a progressive histological manifestation leading to chronic kidney disease (CKD) and associated with mitochondrial dysfunction. In previous work, we showed that Bemcentinib, an Axl receptor tyrosine kinase ...

    • Endothelial microvascular networks affect gene-expression profiles and osteogenic potential of tissue-engineered constructs 

      Pedersen, Torbjørn Østvik; Blois, Anna L; Xing, Zhe; Xue, Ying; Sun, Yang; Finne-Wistrand, Anna; Akslen, Lars A.; Lorens, James B.; Leknes, Knut N.; Fristad, Inge; Mustafa, Kamal Babikeir Eln (Peer reviewed; Journal article, 2013-05-17)
      Introduction: A major determinant of the potential size of cell/scaffold constructs in tissue engineering is vascularization. The aims of this study were twofold: first to determine the in vitro angiogenic and osteogenic ...

    • Enhanced gene expression from retroviral vectors 

      Blø, Magnus; Micklem, David R.; Lorens, James B. (Peer reviewed; Journal article, 2008-02-25)
      Background: Retroviruses are widely used to transfer genes to mammalian cells efficiently and stably. However, genetic elements required for high-level gene expression are incompatible with standard systems. The retroviral ...

    • Evaluating Extracellular Matrix influence on adherent cell signaling by Cold Trypsin Phosphorylation-specific Flow Cytometry 

      Abrahamsen, Iren; Lorens, James B. (Peer reviewed; Journal article, 2013-08-19)
      Background: Tissue microenvironments comprise different extracellular matrix (ECM) proteins that regulate cellular responsiveness to growth factors. In vitro culture of adherent cells on ECM-coated substrata is commonly ...

    • Flow cytometry-based functional selection of RNA interference triggers for efficient epi-allelic analysis of therapeutic targets 

      Micklem, David Robert; Blø, Magnus; Bergström, Petra; Hodneland, Erlend; Tiron, Crina Elena; Høiby, Torill; Gjerdrum, Christine; Hammarsten, Ola; Lorens, James B. (Peer reviewed; Journal article, 2014-06-21)
      Background: The dose-response relationship is a fundamental pharmacological parameter necessary to determine therapeutic thresholds. Epi-allelic hypomorphic analysis using RNA interference (RNAi) can similarly correlate ...

    • High-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia 

      Pelissier, Fanny; Schapiro, Denis; Chang, Hang; Borowsky, Alexander D.; Lee, Jonathan K.; Parvin, Bahram; Stampfer, Martha R.; LaBarge, Mark A.; Bodenmiller, Bernd; Lorens, James B. (Peer reviewed; Journal article, 2018-04)
      Aging is associated with tissue-level changes in cellular composition that are correlated with increased susceptibility to disease. Aging human mammary tissue shows skewed progenitor cell potency, resulting in diminished ...

    • Mesenchymal stem cells induce endothelial cell quiescence and promote capillary formation 

      Pedersen, Torbjørn Østvik; Blois, Anna L; Xue, Ying; Xing, Zhe; Sun, Yang; Finne-Wistrand, Anna; Lorens, James B.; Fristad, Inge; Leknes, Knut N.; Mustafa, Kamal Babikeir Eln (Peer reviewed; Journal article, 2014-02-17)
      Introduction: Rapid establishment of functional blood vessels is a prerequisite for successful tissue engineering. During vascular development, endothelial cells (ECs) and perivascular cells assemble into a complex regulating ...

    • Rapid and reliable cloning of PCR products 

      Lorens, James B. (Peer reviewed; Journal article, 1991-08-08)