Browsing Bergen Open Research Archive by Author "Målen, Hiwa"
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Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway
De Souza, Gustavo Antonio; Leversen, Nils Anders; Målen, Hiwa; Wiker, Harald G (Peer reviewed; Journal article, 2011-12-21)Correct protein compartmentalization is a key step for molecular function and cell viability, and this is especially true for membrane and externalized proteins of bacteria. Recent proteomic reports of Bacillus subtilis ... -
Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains
Målen, Hiwa; De Souza, Gustavo A.; Pathak, Sharad; Søfteland, Tina; Wiker, Harald G. (Peer reviewed; Journal article, 2011-01-24)Background: The potential causes for variation in virulence between distinct M. tuberculosis strains are still not fully known. However, differences in protein expression are probably an important factor. In this study we ... -
Comprehensive Analysis of Exported Proteins from Mycobacterium tuberculosis H37Rv
Målen, Hiwa; Berven, Frode S.; Fladmark, Kari Espolin; Wiker, Harald G. (Proteomics, Peer reviewed; Journal article, 2007-04-21)Proteins secreted by Mycobacterium tuberculosis play an essential role in the pathogenesis of tuberculosis. The culture filtrates of M. tuberculosis H37Rv made by Sadamu Nagai (Japan), are considerably enriched for secreted ... -
Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv
Målen, Hiwa; Pathak, Sharad; Søfteland, Tina; De Souza, Gustavo A.; Wiker, Harald G. (Peer reviewed; Journal article, 2010-04-29)Background Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 ... -
High accuracy mass spectrometry analysis as a tool to verify and improve gene annotation using Mycobacterium tuberculosis as an example
De Souza, Gustavo A.; Målen, Hiwa; Søfteland, Tina; Sælensminde, Gisle; Prasad, Swati; Jonassen, Inge; Wiker, Harald G. (Peer reviewed; Journal article, 2008-07-02)Background: While the genomic annotations of diverse lineages of the Mycobacterium tuberculosis complex are available, divergences between gene prediction methods are still a challenge for unbiased protein dataset generation. ... -
The protein N-alpha-terminal acetyltransferase hNaa10p (hArd1) is phosphorylated in HEK293 cells
Målen, Hiwa; Lillehaug, Johan; Arnesen, Thomas (Peer reviewed; Journal article, 2009-03-02)Background: The hNaa10p (hArd1) protein is the catalytic subunit of the human NatA Nα- terminal acetyltransferase complex. The NatA complex is associated with ribosomes and cotranslationally acetylates human proteins ... -
Proteogenomic Analysis of Polymorphisms and Gene Annotation Divergences in Prokaryotes using a Clustered Mass Spectrometry-Friendly Database
De Souza, Gustavo A.; Arntzen, Magnus Øverlie; Fortuin, Suereta; Schürch, Anita; Målen, Hiwa; McEvoy, Christopher R.; Van Soolingen, Dick; Thiede, Bernd; Warren, Robin M.; Wiker, Harald G. (Peer reviewed; Journal article, 2011-01)Precise annotation of genes or open reading frames is still a difficult task that results in divergence even for data generated from the same genomic sequence. This has an impact in further proteomic studies, and also ... -
Secreted proteins and surface markers on tubercle bacilli
Målen, Hiwa (Doctoral thesis, 2007-12-12)Tuberculosis caused by Mycobacterium tuberculosis (M. tuberculosis) is the major cause of mortality and morbidity of over two million people each year. Mapping of proteins exported by the pathogen to the extracellular ...