Blar i Bergen Open Research Archive på forfatter "Nygaard, Randi Monsen"
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Bruk av massiv parallell sekvensering for påvisning og identifikasjon av mikrober i galle hos pasienter med akutt kolangitt
Nygaard, Randi Monsen (Master thesis, 2017)De fleste infeksjonssykdommer som forårsakes av bakterier og sopp kan behandles med antimikrobielle midler. Kjennskap til hvilke mikrober som forårsaker sykdommene er viktig for å kunne gi pasienten riktig behandling. Den ... -
Characterization of abscesses from liver, pancreas and kidney using deep sequencing of the 16S rRNA gene
Bivand, Joanna Malgorzata; Nygaard, Randi Monsen; Kommedal, Øyvind (Journal article; Peer reviewed, 2021)To characterize the microbial communities in abscess material from liver, pancreas, and kidneys, we performed deep sequencing of the 16S rRNA gene, in addition to cultivation and Sanger based 16S rRNA gene sequencing ... -
Investigating the human jejunal microbiota
Villmones, Heidi Cecilie; Svanevik, Marius; Ulvestad, Elling; Stenstad, Tore; Anthonisen, Inger Lill; Nygaard, Randi Monsen; Dyrhovden, Ruben; Kommedal, Øyvind (Journal article; Peer reviewed, 2022)Descriptions of the small intestinal microbiota are deficient and conflicting. We aimed to get a reliable description of the jejunal bacterial microbiota by investigating samples from two separate jejunal segments collected ... -
Managing Contamination and Diverse Bacterial Loads in 16S rRNA Deep Sequencing of Clinical Samples: Implications of the Law of Small Numbers
Dyrhovden, Ruben; Rippin, Martin; Øvrebø, Kjell Kåre; Nygaard, Randi Monsen; Ulvestad, Elling; Kommedal, Øyvind (Journal article; Peer reviewed, 2021)In this article, we investigate patterns of microbial DNA contamination in targeted 16S rRNA amplicon sequencing (16S deep sequencing) and demonstrate how this can be used to filter background bacterial DNA in diagnostic ...