Blar i Bergen Open Research Archive på forfatter "Pathak, Sharad"
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Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains
Målen, Hiwa; De Souza, Gustavo A.; Pathak, Sharad; Søfteland, Tina; Wiker, Harald G. (Peer reviewed; Journal article, 2011-01-24)Background: The potential causes for variation in virulence between distinct M. tuberculosis strains are still not fully known. However, differences in protein expression are probably an important factor. In this study we ... -
Counting Mycobacteria in Infected Human Cells and Mouse Tissue: A Comparison between qPCR and CFU
Pathak, Sharad; Awuh, Jane A.; Leversen, Nils Anders; Flo, Trude H.; Åsjø, Birgitta (Peer reviewed; Journal article, 2012-04-19)Due to the slow growth rate and pathogenicity of mycobacteria, enumeration by traditional reference methods like colony counting is notoriously time-consuming, inconvenient and biohazardous. Thus, novel methods that rapidly ... -
Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv
Målen, Hiwa; Pathak, Sharad; Søfteland, Tina; De Souza, Gustavo A.; Wiker, Harald G. (Peer reviewed; Journal article, 2010-04-29)Background Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 ... -
Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients
Mustafa, Tehmina; Wergeland, Ida; Baba, Kamaldeen; Pathak, Sharad; Hoosen, Anwar A.; Riise, Anne Margarita Dyrhol (Journal article; Peer reviewed, 2020)Background Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location. Even though mycobacteria are ...