Blar i Bergen Open Research Archive på forfatter "Saghaug, Christina Skår"
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Developing a flow cytometric method to characterize human T cell responses against the enteric protozoan parasite Giardia lamblia
Saghaug, Christina Skår (Master thesis, 2014-06-03)Giardia lamblia is an enteric protozoan parasite, which causes infection in humans worldwide. The impact of the infection varies from asymptomatic carriers to severe disease such as malabsorption syndrome. Evidence for ... -
Genetic diversity in the metronidazole metabolism genes nitroreductases and pyruvate ferredoxin oxidoreductases in susceptible and refractory clinical samples of Giardia lamblia
Saghaug, Christina Skår; Gamlem, Astrid Leinebø; Hauge, Kirsti Brugrand; Vahokoski, Juha; Klotz, Christian; Aebischer, Toni; Langeland, Nina; Hanevik, Kurt (Journal article; Peer reviewed, 2023)The effectiveness of metronidazole against the tetraploid intestinal parasite Giardia lamblia is dependent on its activation/inactivation within the cytoplasm. There are several activating enzymes, including pyruvate ... -
Genetic diversity of the flavohemoprotein gene of Giardia lamblia: Evidence for high allelic heterozygosity and copy number variation
Saghaug, Christina Skår; Klotz, Christian; Kallio, Juha Pekka; Aebischer, Toni; Langeland, Nina; Hanevik, Kurt (Journal article; Peer reviewed, 2020)Purpose: The flavohemoprotein (gFlHb) in Giardia plays an important role in managing nitrosative and oxidative stress, and potentially also in virulence and nitroimidazole drug tolerance. The aim of this study was to analyze ... -
Genetic variability of proteins involved in metronidazole metabolism and detoxification in Giardia lamblia
Saghaug, Christina Skår (Doctoral thesis, 2021-12-09)Metronidazole (MTZ) treatment-refractory Giardia lamblia infections is an emerging global problem. Recent studies show that prevalence rates of refractory infections are 10-20 % and can be as high as 46 %. The recent global ... -
Genetic variation in metronidazole metabolism and oxidative stress pathways in clinical giardia lamblia assemblage A and B isolates
Saghaug, Christina Skår; Klotz, Christian; Kallio, Juha Pekka; Brattbakk, Hans-Richard; Stokowy, Tomasz; Aebischer, Toni; Kursula, Inari; Langeland, Nina; Hanevik, Kurt (Peer reviewed; Journal article, 2019-05-10)Purpose: Treatment-refractory Giardia cases have increased rapidly within the last decade. No markers of resistance nor a standardized susceptibility test have been established yet, but several enzymes and their pathways ... -
Highly contiguous genomes of human clinical isolates of Giardia duodenalis reveal assemblage-and sub-assemblage-specific presence–absence variation in protein-coding genes
Klotz, Christian; Schmid, Marc William; Winter, Katja; Ignatius, Ralf; Weisz, Filip; Saghaug, Christina Skår; Langeland, Nina; Dawson, Scott; Lalle, Marco; Hanevik, Kurt; Cacciò, Simone M.; Aebischer, Toni (Journal article; Peer reviewed, 2023)Giardia duodenalis (syn. G. intestinalis, G. lamblia) is a widespread gastrointestinal protozoan parasite with debated taxonomic status. Currently, eight distinct genetic sub-groups, termed assemblages A–H, are defined ... -
No difference in serum levels of B-cell activating receptor and antibodies against cytolethal distending toxin B and flagellin in post-infectious irritable bowel syndrome and chronic fatigue syndrome after Giardia infection
Hanevik, Kurt; Saghaug, Christina Skår; Aaland, Maren; Mørch, Kristine; Langeland, Nina (Journal article; Peer reviewed, 2022)Background and Aim Functional gastrointestinal disorders (FGIDs) and chronic fatigue syndrome (CFS) frequently occur as comorbid conditions to each other. A shared etiology of these syndromes has been proposed because of ... -
Whole genome sequencing of clinical isolates of Giardia lamblia
Hanevik, Kurt; Bakken, R.; Brattbakk, Hans-Richard; Saghaug, Christina Skår; Langeland, Nina (Peer reviewed; Journal article, 2015-10-29)Clinical isolates from protozoan parasites such as Giardia lamblia are at present practically impossible to culture. By using simple cyst purification methods, we show that Giardia whole genome sequencing of clinical stool ...