Blar i Bergen Open Research Archive på forfatter "Wiker, Harald G"
-
Bacterial proteins with cleaved or uncleaved signal peptides of the general secretory pathway
De Souza, Gustavo Antonio; Leversen, Nils Anders; Målen, Hiwa; Wiker, Harald G (Peer reviewed; Journal article, 2011-12-21)Correct protein compartmentalization is a key step for molecular function and cell viability, and this is especially true for membrane and externalized proteins of bacteria. Recent proteomic reports of Bacillus subtilis ... -
Comparing microbiota profiles in induced and spontaneous sputum samples in COPD patients
Tangedal, Solveig; Aanerud, Marianne; Grønseth, Rune; Drengenes, Christine; Wiker, Harald G; Bakke, Per S.; Eagan, Tomas Mikal (Peer reviewed; Journal article, 2017-08-29)Background: Induced and spontaneous sputum are used to evaluate the airways microbiota. Whether the sputum types can be used interchangeably in microbiota research is unknown. Our aim was to compare microbiota in induced ... -
Coordination of Metabolism and Virulence Factors Expression of Extraintestinal Pathogenic Escherichia coli Purified from Blood Cultures of Patients with Sepsis
Pettersen, Veronika Kucharova; Mosevoll, Knut Anders; Lindemann, Paul Christoffer; Wiker, Harald G (Peer reviewed; Journal article, 2016-09)One of the trademarks of extraintestinal pathogenic Escherichia coli is adaptation of metabolism and basic physiology to diverse host sites. However, little is known how this common human pathogen adapts to permit survival ... -
Diagnosis of extrapulmonary tuberculosis using the MPT64 antigen detection test in a high-income low tuberculosis prevalence setting
Hoel, Ida Marie; Sviland, Lisbeth; Syre, Heidi; Dyrhol-Riise, Anne Ma; Skarstein, Ingerid; Jebsen, Peter Wilhelm; Jørstad, Melissa Davidsen; Wiker, Harald G; Mustafa, Tehmina (Journal article; Peer reviewed, 2020)Background Extrapulmonary tuberculosis (EPTB) poses diagnostic challenges due to the paucibacillary nature of the disease. The immunochemistry-based MPT64 antigen detection test (MPT64 test) has shown promising results ... -
Exploring protocol bias in airway microbiome studies: one versus two PCR steps and 16S rRNA gene region V3 V4 versus V4
Drengenes, Christine; Eagan, Tomas Mikal; Haaland, Ingvild; Wiker, Harald G; Nielsen, Rune (Journal article; Peer reviewed, 2021)Background Studies on the airway microbiome have been performed using a wide range of laboratory protocols for high-throughput sequencing of the bacterial 16S ribosomal RNA (16S rRNA) gene. We sought to determine the ... -
Laboratory contamination in airway microbiome studies
Drengenes, Christine; Wiker, Harald G; Kalananthan, Tharmini; Nordeide, Eli; Eagan, Tomas Mikal; Nielsen, Rune (Peer reviewed; Journal article, 2019-08-14)Background The low bacterial load in samples acquired from the lungs, have made studies on the airway microbiome vulnerable to contamination from bacterial DNA introduced during sampling and laboratory processing. We have ... -
Multiplex Analysis of Pro- or Anti-Inflammatory Serum Cytokines and Chemokines in relation to Gender and Age among Tanzanian Tuberculous Lymphadenitis Patients
Mustafa, Tehmina; Brokstad, Karl Albert; Mfinanga, Sayoki Godfrey M.; Wiker, Harald G (Peer reviewed; Journal article, 2015)Objectives. Tuberculous lymphadenitis is the most common formof extrapulmonary tuberculosis (TB) with a female and paediatric preponderance, postulated to be due to differences in the immune response. The aim of this study ... -
Mycobacterial antigens accumulation in foamy macrophages in murine pulmonary tuberculosis lesions: Association with necrosis and making of cavities
Riaz, Syeda Mariam; Bjune, Gunnar Aksel; Wiker, Harald G; Sviland, Lisbeth; Mustafa, Tehmina (Journal article; Peer reviewed, 2020)Understanding mechanisms of cavitation in tuberculosis (TB) is the missing link that could advance the field towards better control of the infection. Descriptions of human TB suggest that postprimary TB begins as lipid ... -
Protected sampling is preferable in bronchoscopic studies of the airway microbiome
Grønseth, Rune; Drengenes, Christine; Wiker, Harald G; Tangedal, Solveig; Xue, Yaxin; Husebø, Gunnar; Svanes, Øistein; Lehmann, Sverre; Aardal, Marit Elisabeth; Hoang, Tuyen; Kalananthan, Tharmini; Martinsen, Einar Marius Hjellestad; Leiten, Elise Orvedal; Aanerud, Marianne; Nordeide, Eli; Haaland, Ingvild; Jonassen, Inge; Bakke, Per S.; Eagan, Tomas Mikal (Journal article; Peer reviewed, 2017)The aim was to evaluate susceptibility of oropharyngeal contamination with various bronchoscopic sampling techniques. 67 patients with obstructive lung disease and 58 control subjects underwent bronchoscopy with ... -
Rapid and Specific Diagnosis of Extrapulmonary Tuberculosis by Immunostaining of Tissues and Aspirates With Anti-MPT64
Purohit, Manju; Sviland, Lisbeth; Wiker, Harald G; Mustafa, Tehmina (Peer reviewed; Journal article, 2017-04)Background: Extrapulmonary tuberculosis (EPTB) constitutes about 15% to 20% of all cases of tuberculosis (TB). The confirmation of EPTB has always been a challenge to laboratory personnel. We aim to evaluate the diagnostic ... -
Sputum microbiota and inflammation at stable state and during exacerbations in a cohort of chronic obstructive pulmonary disease (COPD) patients
Tangedal, Solveig; Nielsen, Rune; Aanerud, Marianne; Persson, Louise Jeanette Pauline; Wiker, Harald G; Bakke, Per S.; Hiemstra, Pieter S.; Eagan, Tomas Mikal (Peer reviewed; Journal article, 2019-09-17)Background: Exacerbations of chronic obstructive pulmonary disease (COPD) are debilitating events and spur disease progression. Infectious causes are frequent; however, it is unknown to what extent exacerbations are caused ...