Blar i Bergen Open Research Archive på forfatter "Wiker, Harald G."
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Comparison of membrane proteins of Mycobacterium tuberculosis H37Rv and H37Ra strains
Målen, Hiwa; De Souza, Gustavo A.; Pathak, Sharad; Søfteland, Tina; Wiker, Harald G. (Peer reviewed; Journal article, 2011-01-24)Background: The potential causes for variation in virulence between distinct M. tuberculosis strains are still not fully known. However, differences in protein expression are probably an important factor. In this study we ... -
Comprehensive Analysis of Exported Proteins from Mycobacterium tuberculosis H37Rv
Målen, Hiwa; Berven, Frode S.; Fladmark, Kari Espolin; Wiker, Harald G. (Proteomics, Peer reviewed; Journal article, 2007-04-21)Proteins secreted by Mycobacterium tuberculosis play an essential role in the pathogenesis of tuberculosis. The culture filtrates of M. tuberculosis H37Rv made by Sadamu Nagai (Japan), are considerably enriched for secreted ... -
Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv
Målen, Hiwa; Pathak, Sharad; Søfteland, Tina; De Souza, Gustavo A.; Wiker, Harald G. (Peer reviewed; Journal article, 2010-04-29)Background Membrane- and membrane-associated proteins are important for the pathogenicity of bacteria. We have analysed the content of these proteins in virulent Mycobacterium tuberculosis H37Rv using Triton X-114 ... -
Differential in vivo expression of mycobacterial antigens in Mycobacterium tuberculosis infected lungs and lymph node tissues
Mustafa, Tehmina; Leversen, Nils Anders; Sviland, Lisbet; Wiker, Harald G. (Peer reviewed; Journal article, 2014-10-03)Background: The clinical course of tuberculosis (TB) infection, bacterial load and the morphology of lesions vary between pulmonary and extrapulmonary TB. Antigens expressed in abundance during infection could represent ... -
Effect of Non-tuberculous Mycobacteria on Host Biomarkers Potentially Relevant for Tuberculosis Management.
Dhanasakeran, S.; Jenum, Synne; Stavrum, Ruth; Wiker, Harald G.; Kenneth, John; Vaz, Mario; Doherty, Tanya Mark; Grewal, Harleen (Peer reviewed; Journal article, 2014-10-16)Background Non-tuberculous mycobacteria (NTM) are different from Mycobacterium tuberculosis (MTB) both in their ubiquitous environmental distribution and in their reduced capacity to cause disease. While often neglected ... -
Evidence for waning of latency in a cohort study of tuberculosis
Wiker, Harald G.; Mustafa, Tehmina; Bjune, Gunnar Aksel; Harbo, Morten (Peer reviewed; Journal article, 2010-02-23)Background To investigate how the risk of active tuberculosis disease is influenced by time since original infection and to determine whether the risk of reactivation of tuberculosis increases or decreases with age. Methods ... -
High accuracy mass spectrometry analysis as a tool to verify and improve gene annotation using Mycobacterium tuberculosis as an example
De Souza, Gustavo A.; Målen, Hiwa; Søfteland, Tina; Sælensminde, Gisle; Prasad, Swati; Jonassen, Inge; Wiker, Harald G. (Peer reviewed; Journal article, 2008-07-02)Background: While the genomic annotations of diverse lineages of the Mycobacterium tuberculosis complex are available, divergences between gene prediction methods are still a challenge for unbiased protein dataset generation. ... -
Immunohistochemical diagnosis of abdominal and lymph node tuberculosis by detecting Mycobacterium tuberculosis complex specific antigen MPT64
Purohit, Manju Raj; Mustafa, Tehmina; Wiker, Harald G.; Mørkve, Odd; Sviland, Lisbet (Peer reviewed; Journal article, 2007-09-25)Background: The aim of this study was to evaluate the diagnostic potential of immunohistochemistry using an antibody to the secreted mycobacterial antigen MPT64, in abdominal and lymph node tuberculosis. Methods: We used ... -
Proteogenomic Analysis of Polymorphisms and Gene Annotation Divergences in Prokaryotes using a Clustered Mass Spectrometry-Friendly Database
De Souza, Gustavo A.; Arntzen, Magnus Øverlie; Fortuin, Suereta; Schürch, Anita; Målen, Hiwa; McEvoy, Christopher R.; Van Soolingen, Dick; Thiede, Bernd; Warren, Robin M.; Wiker, Harald G. (Peer reviewed; Journal article, 2011-01)Precise annotation of genes or open reading frames is still a difficult task that results in divergence even for data generated from the same genomic sequence. This has an impact in further proteomic studies, and also ... -
Proteogenomics in Microbiology: Taking the Right Turn at the Junction of Genomics and Proteomics
Kucharova, Veronika; Wiker, Harald G. (Peer reviewed; Journal article, 2014-12)High-accuracy and high-throughput proteomic methods have completely changed the way we can identify and characterize proteins. MS-based proteomics can now provide a unique supplement to genomic data and add a new level of ... -
Proteomic analysis of total cellular proteins of human neutrophils
Tomazella, Gisele G.; da Silva, Idalete; Laure, Helen J.; Rosa, José C.; Chammas, Roger; Wiker, Harald G.; de Souza, Gustavo A.; Greene, Lewis J. (Peer reviewed; Journal article, 2009-08-31)Background: Neutrophils are the most abundant leukocytes in peripheral blood and represent one of the most important elements of innate immunity. Recent subcellular proteomic studies have focused on the identification of ... -
Serodiagnosis of tuberculous lymphadenitis using a combination of antigens
Beyene, Demissew Balcha; Franken, Kees Lumc; Yamuah, Lawrence; Aseffa, Abraham; Wiker, Harald G.; Kolk, Arend; Engers, Howard; Klatser, Paul R.; Sviland, Lisbet (Peer reviewed; Journal article, 2010)Background: The diagnosis of extra-pulmonary tuberculosis (EPTB) by conventional methods such as culture and microscopy has low sensitivity and requires an invasive procedure. A simple rapid serological test would be of ...