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dc.contributor.authorGillespie, Emily L.
dc.contributor.authorMadsen-McQueen, Tesa
dc.contributor.authorEriksson, Torsten
dc.contributor.authorBailey, Adam
dc.contributor.authorMurrell, Zack E
dc.date.accessioned2021-04-20T09:20:08Z
dc.date.available2021-04-20T09:20:08Z
dc.date.created2019-12-01T21:24:26Z
dc.date.issued2019
dc.PublishedApplications in Plant Sciences. 2019, 7 (6): e11267.en_US
dc.identifier.issn2168-0450
dc.identifier.urihttps://hdl.handle.net/11250/2738565
dc.description.abstractPremise: Microsatellite markers were developed for sandmyrtle, Kalmia buxifolia (Ericaceae), to facilitate phylogeographic studies in this taxon and possibly many of its close relatives. Methods and Results: Forty‐eight primer pairs designed from paired‐end Illumina MiSeq data were screened for robust amplification. Sixteen pairs were amplified again, but with fluorescently labeled primers to facilitate genotyping. Resulting chromatograms were evaluated for variability using three populations from Tennessee, North Carolina, and New Jersey, USA. Eleven primer pairs were reliable and polymorphic (mean 3.92 alleles), one was reliable but monomorphic, and four were not reliable. The markers exhibited lower heterozygosity (mean 0.246) than expected (mean 0.464). Cross‐amplification in the remaining nine Kalmia species exhibited a phylogenetic pattern, suggesting broad applicability of the markers across the genus. Conclusions: These microsatellite markers will be useful in population genetics and species boundaries studies of K. buxifolia, K. procumbens, and likely all other Kalmia species.en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleMicrosatellite markers for the biogeographically enigmatic sandmyrtle (Kalmia buxifolia, Phyllodoceae: Ericaceae)en_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright 2019 Gillespie et al.en_US
dc.source.articlenumbere11267en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.1002/aps3.11267
dc.identifier.cristin1755159
dc.source.journalApplications in Plant Sciencesen_US
dc.source.407en_US
dc.source.146en_US
dc.identifier.citationApplications in Plant Sciences. 2019, 7 (6), e11267.


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal