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dc.contributor.authorHong, Yu
dc.contributor.authorLiang, Kristina Xiao
dc.contributor.authorGilhus, Nils Erik
dc.date.accessioned2021-04-20T13:17:42Z
dc.date.available2021-04-20T13:17:42Z
dc.date.created2020-07-29T13:20:08Z
dc.date.issued2020-07-08
dc.PublishedScientific Reports. 2020, 10:11230 1-13.
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/11250/2738704
dc.description.abstractAcetylcholine receptor (AChR) antibodies are the most important pathogenic marker in patients with myasthenia gravis (MG). The antibodies bind to AChRs on the postsynaptic membrane, and this leads to receptor degradation, destruction, or functional blocking with impaired signal at the neuromuscular junction. In this study, we have explored the effects of AChR antibodies binding to mature human myotubes with agrin-induced AChR clusters and pathways relevant for AChR degradation using bulk RNA sequencing. Protein-coding RNAs and lncRNAs were examined by RNA sequencing analysis. AChR antibodies induced marked changes of the transcriptomic profiles, with over 400 genes differentially expressed. Cholesterol metabolic processes and extracellular matrix organization gene sets were influenced and represent AChR-trafficking related pathways. Muscle contraction and cellular homeostasis gene sets were also affected, and independently of AChR trafficking. Furthermore, we found changes in a protein-coding RNA and lncRNA network, where expression of lncRNA MEG3 correlated closely with protein-coding genes for cellular homeostasis. We conclude that AChR antibodies induce an active response in human skeletal muscle cells which affects key intra- and extracellular pathways.en_US
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleAChR antibodies show a complex interaction with human skeletal muscle cells in a transcriptomic studyen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright The Author(s) 2020en_US
dc.source.articlenumber11230en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.1038/s41598-020-68185-x
dc.identifier.cristin1820903
dc.source.journalScientific Reportsen_US
dc.source.4010:11230
dc.source.pagenumber1-13en_US
dc.identifier.citationScientific Reports. 2020, 10 (11230)en_US
dc.source.volume10en_US


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