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dc.contributor.authorKoivunen, Jarkko
dc.contributor.authorTu, Hongmin
dc.contributor.authorKemppainen, Antti
dc.contributor.authorAnbazhagan, Padmanabhan
dc.contributor.authorFinnilä, Mikko A
dc.contributor.authorSaarakkala, Simo
dc.contributor.authorKäpylä, Jarmo
dc.contributor.authorLu, Ning
dc.contributor.authorHeikkinen, Anne
dc.contributor.authorJuffer, André H
dc.contributor.authorHeino, Jyrki
dc.contributor.authorGullberg, Donald
dc.contributor.authorTaina, Pihlajaniemi
dc.description.abstractCollagen XIII is a conserved transmembrane collagen mainly expressed in mesenchymal tissues. Previously, we have shown that collagen XIII modulates tissue development and homeostasis. Integrins are a family of receptors that mediate signals from the environment into the cells and vice versa. Integrin α11β1 is a collagen receptor known to recognize the GFOGER (O=hydroxyproline) sequence in collagens. Interestingly, collagen XIII and integrin α11β1 both have a role in the regulation of bone homeostasis. To study whether α11β1 is a receptor for collagen XIII, we utilized C2C12 cells transfected to express α11β1 as their only collagen receptor. The interaction between collagen XIII and integrin α11β1 was also confirmed by surface plasmon resonance and pull-down assays. We discovered that integrin α11β1 mediates cell adhesion to two collagenous motifs, namely GPKGER and GF(S)QGEK, that were shown to act as the recognition sites for the integrin α11-I domain. Furthermore, we studied the in vivo significance of the α11β1-collagen XIII interaction by crossbreeding α11 null mice (Itga11−/−) with mice overexpressing Col13a1 (Col13a1oe). When we evaluated the bone morphology by microcomputed tomography, Col13a1oe mice had a drastic bone overgrowth followed by severe osteoporosis, whereas the double mutant mouse line showed a much milder bone phenotype. To conclude, our data identifies integrin α11β1 as a new collagen XIII receptor and demonstrates that this ligand-receptor pair has a role in the maintenance of bone homeostasis.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleIntegrin α11β1 is a receptor for collagen XIIIen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.rights.holderCopyright The Author(s) 2020en_US
dc.source.journalCell and Tissue Researchen_US
dc.relation.projectDirektoratet for internasjonalisering og kvalitetsutvikling i høgare utdanning: NNA-2016/10026en_US
dc.relation.projectNorges forskningsråd: 223250en_US
dc.identifier.citationCell and Tissue Research. 2021, 383, 1135-1153.en_US

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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal