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dc.contributor.authorGleadall, Nicholas S.
dc.contributor.authorVeldhuisen, Barbera
dc.contributor.authorGollub, Jeremy
dc.contributor.authorButterworth, Adam S.
dc.contributor.authorOrd, John
dc.contributor.authorPenkett, Christopher J.
dc.contributor.authorTimmer, Tiffany C.
dc.contributor.authorSauer, Carolin M.
dc.contributor.authorVan Der Bolt, Nieke
dc.contributor.authorBrown, Colin
dc.contributor.authorBrügger, Kim
dc.contributor.authorDilthey, Alexander T.
dc.contributor.authorDuarte, Daniel
dc.contributor.authorGrimsley, Shane
dc.contributor.authorVan Den Hurk, Katja
dc.contributor.authorJongerius, John M.
dc.contributor.authorLuken, Jessie
dc.contributor.authorMegy, Karyn
dc.contributor.authorMiflin, Gail
dc.contributor.authorNelson, Christopher S.
dc.contributor.authorPrinsze, Femmeke J.
dc.contributor.authorSambrook, Jennifer
dc.contributor.authorSimeoni, Ilenia
dc.contributor.authorSweeting, Michael
dc.contributor.authorThornton, Nicole
dc.contributor.authorTrompeter, Sara
dc.contributor.authorTuna, Salih
dc.contributor.authorVarma, Ram
dc.contributor.authorWalker, Matthew R.
dc.contributor.authorDanesh, John
dc.contributor.authorRoberts, David J.
dc.contributor.authorOuwehand, Willem H.
dc.contributor.authorStirrups, Kathleen E.
dc.contributor.authorRendon, Augusto
dc.contributor.authorWesthoff, Connie M.
dc.contributor.authorDi Angelantonio, Emanuele
dc.contributor.authorvan der Schoot, C. Ellen
dc.contributor.authorAstle, William J.
dc.contributor.authorWatkins, Nicholas A.
dc.contributor.authorLane, William J.
dc.date.accessioned2021-07-08T13:34:44Z
dc.date.available2021-07-08T13:34:44Z
dc.date.created2020-09-29T12:04:09Z
dc.date.issued2020
dc.identifier.issn2473-9529
dc.identifier.urihttps://hdl.handle.net/11250/2763961
dc.description.abstractEach year, blood transfusions save millions of lives. However, under current blood-matching practices, sensitization to non–self-antigens is an unavoidable adverse side effect of transfusion. We describe a universal donor typing platform that could be adopted by blood services worldwide to facilitate a universal extended blood-matching policy and reduce sensitization rates. This DNA-based test is capable of simultaneously typing most clinically relevant red blood cell (RBC), human platelet (HPA), and human leukocyte (HLA) antigens. Validation was performed, using samples from 7927 European, 27 South Asian, 21 East Asian, and 9 African blood donors enrolled in 2 national biobanks. We illustrated the usefulness of the platform by analyzing antibody data from patients sensitized with multiple RBC alloantibodies. Genotyping results demonstrated concordance of 99.91%, 99.97%, and 99.03% with RBC, HPA, and HLA clinically validated typing results in 89 371, 3016, and 9289 comparisons, respectively. Genotyping increased the total number of antigen typing results available from 110 980 to >1 200 000. Dense donor typing allowed identification of 2 to 6 times more compatible donors to serve 3146 patients with multiple RBC alloantibodies, providing at least 1 match for 176 individuals for whom previously no blood could be found among the same donors. This genotyping technology is already being used to type thousands of donors taking part in national genotyping studies. Extraction of dense antigen-typing data from these cohorts provides blood supply organizations with the opportunity to implement a policy of genomics-based precision matching of blood.en_US
dc.language.isoengen_US
dc.publisherAmerican Society of Hematologyen_US
dc.titleDevelopment and validation of a universal blood donor genotyping platform: A multinational prospective studyen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionpublishedVersionen_US
dc.rights.holderCopyright American Society of Hematologyen_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.1182/bloodadvances.2020001894
dc.identifier.cristin1834797
dc.source.journalBlood Advancesen_US
dc.source.pagenumber3495-3506en_US
dc.identifier.citationBlood Advances. 2020, 4 (15), 3495-3506.en_US
dc.source.volume4en_US
dc.source.issue15en_US


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